公开(公告)号：US08367337B2

公开(公告)日：2013-02-05

申请号：US12339471

申请日：2008-12-19

申请人： Corinne Jay ,  Dianne Van Strijp ,  Paul Van de Wiel ,  Birgit Deiman

发明人： Corinne Jay ,  Dianne Van Strijp ,  Paul Van de Wiel ,  Birgit Deiman

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/689 ,  C12Q1/6844 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/16

摘要： The present invention provides improved tests for the detection of methicillin-resistant Staphylococcus aureus. The tests are particularly useful for eliminating false positive results due to the presence of a mixed bacterial population in patient samples.

公开(公告)号：US5536645A

公开(公告)日：1996-07-16

申请号：US353360

申请日：1994-12-02

申请人： Corinne Jay

发明人： Corinne Jay

IPC分类号： C12Q1/04 ,  C12N1/20 ,  C12N1/32 ,  C12Q1/18

CPC分类号： C12Q1/045

摘要： The invention relates to a nutritive medium for the culture of microorganisms, containing alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, biotin, calcium pantothenate, folic acid, inositol, nicotinamide, vitamin B6, thiamine chloride, lipoic acid, choline, ethyl oxaloacetate, spermidine, Tween 80, purine and pyrimidine nucleosides, glucose, malic acid, iron, potassium, magnesium, calcium, sodium, chloride, phosphate ion, ammonium ion, acidic buffer, and basic buffer. The invention also relates to the use of such a medium.

摘要翻译： 本发明涉及含有丙氨酸，精氨酸，天冬酰胺，天冬氨酸，半胱氨酸，谷氨酰胺，谷氨酸，甘氨酸，组氨酸，异亮氨酸，亮氨酸，赖氨酸，甲硫氨酸，苯丙氨酸，脯氨酸，丝氨酸，苏氨酸的微生物培养的营养培养基， 柠檬酸，维生素B6，硫胺素氯化物，硫辛酸，胆碱，草酰乙酸乙酯，亚精胺，吐温80，嘌呤和嘧啶核苷，葡萄糖，苹果酸，铁，钾 ，镁，钙，钠，氯化物，磷酸根离子，铵离子，酸性缓冲液和碱性缓冲液。 本发明还涉及这种介质的用途。

公开(公告)号：US07723095B2

公开(公告)日：2010-05-25

申请号：US10343109

申请日：2001-07-26

申请人： Philippe Cleuziat ,  Sandra Incardona ,  Corinne Jay

发明人： Philippe Cleuziat ,  Sandra Incardona ,  Corinne Jay

IPC分类号： C12N1/00

CPC分类号： C12N1/06 ,  C12M47/06

摘要： A method for lysing prokaryotic or eukaryotic cells, or for simultaneous lysis of both, which includes at least three of the following: a mass of active, small-diameter beads corresponding to 50% or less than a mass of active, larger-diameter beads, and/or a total mass of lysing beads (consisting of a single type of bead or a mixture of smaller and larger beads) corresponding to between 50 and 100% of the total mass of the processed biological sample, and/or a lysis time of from 10 to 20 minutes, and/or seven or less non-lysing glass beads to drive the movement of the lysing beads, and/or from five to fifteen non-lysing iron beads to drive the movement of the lysing beads, depending on whether sonication, mechanical vortex centrifugation or magnetic vortex centrifugation is used.

摘要翻译： 用于裂解原核或真核细胞或同时裂解两者的方法，其包括以下至少三种：大量活性的小直径珠子，其对应于活性较大直径珠粒的质量的50％或更小 ，和/或相当于处理的生物样品总质量的50-100％的裂解珠（由单一类型的珠粒或较小和较大珠粒的混合物组成）的总质量，和/或裂解时间 10分钟至20分钟，和/或7个或更少的非裂解玻璃珠，以驱动裂解珠的移动，和/或5至15个非裂解铁珠以驱动溶胞珠的运动，这取决于 是否使用超声处理，机械涡流离心或磁涡旋离心。

公开(公告)号：US07659059B2

公开(公告)日：2010-02-09

申请号：US10560433

申请日：2004-07-08

申请人： Alain Troesch ,  Bruno Lacroix ,  Corinne Jay

发明人： Alain Troesch ,  Bruno Lacroix ,  Corinne Jay

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689

摘要： The present invention relates to a method for detecting and/or identifying bacteria of the genus Staphylococcus, comprising the following steps: A. the nucleic acid material of the bacteria of the genus Staphylococcus is extracted, B. at least one target sequence of the nucleic acid material of the bacteria of the genus Staphylococcus is amplified using at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 1 and/or at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 2, in order to obtain amplicons of the target sequence, C. the presence of bacteria of the genus Staphylococcus is determined by detecting said amplicons.

摘要翻译： 本发明涉及一种用于检测和/或鉴定葡萄球菌属细菌的方法，包括以下步骤：A.提​​取葡萄球菌属细菌的核酸物质，B.核酸的至少一个靶序列 使用至少一个包含SEQ ID No.1的至少10个核苷酸基序的扩增引物和/或至少一个包含SEQ ID No.2的至少10个核苷酸基序的扩增引物扩增葡萄球菌属细菌的酸性物质， 以获得靶序列的扩增子，C.通过检测所述扩增子来确定葡萄球菌属细菌的存在。

公开(公告)号：US20070292846A1

公开(公告)日：2007-12-20

申请号：US10560433

申请日：2004-07-08

申请人： Alain Troesch ,  Bruno Lacroix ,  Corinne Jay

发明人： Alain Troesch ,  Bruno Lacroix ,  Corinne Jay

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/689

摘要： The present invention relates to a method for detecting and/or identifying bacteria of the genus Staphylococcus, comprising the following steps: A. the nucleic acid material of the bacteria of the genus Staphylococcus is extracted, B. at least one target sequence of the nucleic acid material of the bacteria of the genus Staphylococcus is amplified using at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 1 and/or at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 2, in order to obtain amplicons of the target sequence, C. the presence of bacteria of the genus Staphylococcus is determined by detecting said amplicons.

摘要翻译： 本发明涉及一种用于检测和/或鉴定葡萄球菌属细菌的方法，包括以下步骤：A.提​​取葡萄球菌属细菌的核酸物质，B.核酸的至少一个靶序列 使用至少一个包含SEQ ID No.1的至少10个核苷酸基序的扩增引物和/或至少一个包含SEQ ID No.2的至少10个核苷酸基序的扩增引物扩增葡萄球菌属细菌的酸性物质， 以获得靶序列的扩增子，C.通过检测所述扩增子来确定葡萄球菌属细菌的存在。

公开(公告)号：US20090203013A1

公开(公告)日：2009-08-13

申请号：US12339471

申请日：2008-12-19

申请人： Corinne Jay ,  Dianne Van Strijp ,  Paul Van de Wiel ,  Birgit Deiman

发明人： Corinne Jay ,  Dianne Van Strijp ,  Paul Van de Wiel ,  Birgit Deiman

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q1/6844 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/16

摘要： The present invention provides improved tests for the detection of methicillin-resistant Staphylococcus aureus. The tests are particularly useful for eliminating false positive results due to the presence of a mixed bacterial population in patient samples.

摘要翻译： 本发明提供了用于检测耐甲氧西林金黄色葡萄球菌的改进试验。 由于在患者样品中存在混合细菌群，这些检测对于消除假阳性结果特别有用。

公开(公告)号：US06660472B1

公开(公告)日：2003-12-09

申请号：US09509006

申请日：2000-07-17

申请人： Lise Santoro ,  Sophie Lopez ,  Bruno Colin ,  Corinne Jay ,  Cécile Paris ,  Kathleen Ann Clark Dickey

发明人： Lise Santoro ,  Sophie Lopez ,  Bruno Colin ,  Corinne Jay ,  Cécile Paris ,  Kathleen Ann Clark Dickey

IPC分类号： C12Q168

CPC分类号： C12N1/066 ,  Y10T436/143333

摘要： The invention concerns a lysis method for a biological sample comprising at least one micro-organism of the bacteria type, for releasing a nucleic material of interest belonging to said micro-organism, which consists in: providing in a container a biological sample in liquid medium; providing in said container at least one particulate material, relatively hard, and substantially inert with respect to the nucleic material; submitting the biological sample and particulate material mixture to a movement. The invention is characterised in that in combination, the selected movement is of the vortex type, and satisfies the following conditions: the particulate material consists of beads with diameter between 90 and 150 &mgr;m; and the apparent volume of the beads (Vb) and the volume of the liquid sample (Ve) are linked by the relationship Ve=&agr;. Vb, with &agr; ranging between 1.4 and 10 when the container is tubular in shape, and &agr; is not more than 2.1 when the container is in the shape of a disk; under such conditions, without any addition of reagent and/or additional process, the method consists in releasing directly into the liquid medium the nucleic material in native state and accessible to any reagent in a subsequent process.

摘要翻译： 本发明涉及包含至少一种细菌类微生物的生物样品的裂解方法，用于释放属于所述微生物的感兴趣的核酸，其包括：在容器中提供液体培养基中的生物样品 ; 在所述容器中提供相对于所述核材料相对较硬且基本上惰性的至少一种颗粒材料; 将生物样品和颗粒物质混合物提交到运动中。 本发明的特征在于，所选择的运动是涡流型的，并且满足以下条件：颗粒材料由直径在90至150μm之间的珠粒组成; 并且珠子（Vb）的表观体积和液体样品（Ve）的体积通过关系Ve =α连接。 Vb，当容器为管状形状时，α范围在1.4和10之间，当容器为盘形时，α不大于2.1; 在这种条件下，没有任何添加试剂和/或另外的方法，该方法包括将天然状态的核酸材料直接释放到液体介质中，并在随后的方法中可用于任何试剂。