公开(公告)号：US11355334B2

公开(公告)日：2022-06-07

申请号：US16312009

申请日：2017-06-20

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/06 ,  H01J49/00

Abstract: Methods and systems are provided herein for selectively removing product ions resulting from an ECD dissociation event from the interaction region of an ECD reaction cell, while other precursor peptide ions continue to undergo ECD within the interaction region, thereby reducing or preventing the occurrence of multiple electron capture events by the product ions. In some aspects, the preferential extraction of product ions from the interaction region during the ECD reaction can occur without an auxiliary AC field being generated within the interaction region. Additionally, in some aspects, the methods and systems disclosed herein can subject the various product ions to a non-dissociative charge reduction via exposure to reagent ions of the opposite polarity so as to selectively concentrate product ions to a lower charge state.

公开(公告)号：US10256088B2

公开(公告)日：2019-04-09

申请号：US15531505

申请日：2015-10-14

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/00 ,  H01J49/42

Abstract: An RF voltage is applied across each electrode of a first array of evenly spaced, parallel, and coplanar electrodes and its corresponding electrode of a second array of evenly spaced, parallel, and coplanar electrodes. The RF voltage varies in amplitude according to an RF voltage amplitude gradient. The RF voltage produces an array of different quadrupole RF electric fields in a uniform gap between the first array and the second array. A DC voltage is superimposed on each electrode of the first array and its corresponding electrode of the second array. The DC voltage varies according to a DC voltage gradient in order to produce a DC electric field in the uniform gap. When ions are introduced in the uniform gap, the DC electric field causes the ions to drift toward quadrupole RF electric fields with increasing RF voltage amplitudes where the ions are trapped according to their m/z.

公开(公告)号：US10062556B2

公开(公告)日：2018-08-28

申请号：US15540750

申请日：2015-12-21

Applicant: DH Technologies Development PTE Ltd.

Inventor： Takashi Baba

IPC: H01J49/42 ,  H01J49/26 ,  H01J49/00 ,  H01J49/06

CPC classification number: H01J49/0072 ,  H01J49/0054 ,  H01J49/062 ,  H01J49/063

Abstract: A method and apparatus for conducting reactions between precursor ions and reagent ions, for example, a reaction between a precursor cation and an electron, such as ECD, are disclosed. The apparatus comprises first, second, and third pathways, each of which extends at least partially along a central axis, and wherein the second central axis is orthogonal to the first and third central axes. Charged species can be introduced into the second pathway as the ions are transmitted therethrough, thereby increasing precursor ion and charged species interaction without simultaneous trapping of the species.

公开(公告)号：US20170263431A1

公开(公告)日：2017-09-14

申请号：US15531505

申请日：2015-10-14

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/42

CPC classification number: H01J49/4225 ,  H01J49/4205 ,  H01J49/4255

Abstract: An RF voltage is applied across each electrode of a first array of evenly spaced, parallel, and coplanar electrodes and its corresponding electrode of a second array of evenly spaced, parallel, and coplanar electrodes. The RF voltage varies in amplitude according to an RF voltage amplitude gradient. The RF voltage produces an array of different quadrupole RF electric fields in a uniform gap between the first array and the second array. A DC voltage is superimposed on each electrode of the first array and its corresponding electrode of the second array. The DC voltage varies according to a DC voltage gradient in order to produce a DC electric field in the uniform gap. When ions are introduced in the uniform gap, the DC electric field causes the ions to drift toward quadrupole RF electric fields with increasing RF voltage amplitudes where the ions are trapped according to their m/z.

公开(公告)号：US20170117124A1

公开(公告)日：2017-04-27

申请号：US15316915

申请日：2015-06-04

Applicant: DH Technologies Development PTE Ltd.

Inventor： Takashi Baba ,  John Lawrence Campbell

IPC: H01J49/00 ,  H01J49/42

CPC classification number: H01J49/0054 ,  G01N33/92 ,  H01J49/0072 ,  H01J49/4225

Abstract: A method and apparatus for analyzing samples using mass spectrometry are disclosed. The apparatus includes a reaction device configured to dissociate sample ions into fragments by reacting the sample ions with a charged species (e.g., electrons) such as through ECD, EID, or EIEIO. The kinetic energy of the charged species is such that the fragments may be detected and produce spectra that allow for the determination of isomeric species in the sample and the location of double bonds of sample molecules. The fragments may include radical fragments and non-radical fragments. The apparatus may also include an oxygen gas source configured to react with the radical fragments to produce oxygen-radical fragments. Spectra resulting from analysis of the fragments may allow for the determination of the oxygen-radical fragments resulting from the dissociation of the sample molecules.

公开(公告)号：US20160217991A1

公开(公告)日：2016-07-28

申请号：US15026235

申请日：2014-10-07

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba

IPC: H01J49/04

CPC classification number: H01J49/0036 ,  H01J49/0031 ,  H01J49/004 ,  H01J49/04 ,  H01J49/067 ,  H01J49/4285

Abstract: Systems and methods for multiplexed precursor ion selection are provided. A mass isolator includes a selection region of rods, a transmission region of rods, and a barrier electrode lens separating the selection and transmission regions. Two or more different precursor ions are selected by applying two or more different AC voltage frequencies to rods of a selection region in order to resonate the two or more different precursor ions from a continuous beam of ions. The two or more different precursor ions are transmitted by applying a DC voltage to the barrier electrode lens, creating an electric field potential barrier over which only the resonating ions are transmitted. Precursor ions of product ions from combined product ion spectra produced by multiplexed precursor ion selection are identified by grouping the target precursor ions.

Abstract translation: 提供了多路前体离子选择的系统和方法。 质量隔离器包括杆的选择区域，杆的透射区域和分离选择和透射区域的阻挡电极透镜。 通过对选择区域的棒施加两个或更多个不同的AC电压频率来选择两个或更多个不同的前体离子，以便使两个或更多个不同的前体离子与连续离子束共振。 通过向阻挡电极透镜施加DC电压来传输两种或更多种不同的前体离子，产生仅在其上传播谐振离子的电场势垒。 通过将目标前体离子分组来鉴定由多重前体离子选择产生的组合产物离子光谱的产物离子的前体离子。

公开(公告)号：US09305757B2

公开(公告)日：2016-04-05

申请号：US14369344

申请日：2012-12-06

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/42 ,  H01J49/26 ,  H01J49/06

CPC classification number: H01J49/06 ,  H01J49/26 ,  H01J49/4225 ,  H01J49/4255

Abstract: A method is provided for processing ions in a multipole ion trap, comprising generating RF radial confinement fields within a first and second multipole rod set positioned in tandem, a ratio of q value exhibited by the second rod set relative to the first rod set being greater than one for any m/z, said RF axial confinement fields within the first and second rod sets interacting in an interaction region between the first and second rod sets so as to produce a fringing field; transmitting ions through said first rod set towards said second rod set; and increasing the radial oscillation amplitude of at least a portion of the ions within said first rod set such that at least a portion of said ions having an increased radial oscillation amplitude are repulsed by said fringing field.

Abstract translation: 提供了一种用于处理多极离子阱中的离子的方法，包括在串联定位的第一和第二多极棒组内产生RF径向限制场，第二棒组相对于第一棒组显示的q值的比值更大 所述第一和第二杆组内的所述RF轴向限制场在所述第一和第二杆组之间的相互作用区域中相互作用以产生边缘场; 将离子通过所述第一杆组传递到所述第二杆组; 并且增加所述第一棒组中的离子的至少一部分的径向振荡振幅，使得具有增加的径向振荡幅度的所述离子的至少一部分被所述边缘场排斥。

公开(公告)号：US12188944B2

公开(公告)日：2025-01-07

申请号：US17440130

申请日：2020-05-13

Applicant: DH Technologies Development PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin

IPC: G01N33/68 ,  H01J49/42

Abstract: Method and devices for performing top down analysis of an antibody are described which involve utilizing an ion source to generate a plurality of ions from a sample containing at least one intact antibody. Further, transmitting said plurality of ions through a quadrupole rod set while applying an RF signal thereto and in the absence of a resolving DC voltage so as to preferentially transmit precursor ions having an m/z value greater than a low mass cutoff of about 1500 m/z from the quadrupole rod set to an ECD cell. The method and device can also performing an ECD reaction in the ECD cell on said precursor ions and also detect reaction products from the ECD reaction.

公开(公告)号：US11551919B2

公开(公告)日：2023-01-10

申请号：US17284321

申请日：2019-10-08

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin

IPC: H01J49/00 ,  H01J49/06

Abstract: An electron-ion interaction module for use in a mass spectrometer, having a plurality of rod sets arranged relative to one another such that said rod sets share a common longitudinal axis and each of said rod sets is longitudinally separated from an adjacent rod set by a gap, each of said rod sets comprising a plurality of rods arranged around said common longitudinal axis. The module further includes at least one magnet disposed around said rod sets so as to at least partially surround one or more of said plurality of rod sets and configured to generate a static magnetic field along said longitudinal axis. The rod sets are configured to receive electrons from an electron source and ions from an ion source within an interaction volume defined by the rods. One or more RF voltage sources coupled to the plurality of rod sets applies voltages to the rods.

公开(公告)号：US20220399198A1

公开(公告)日：2022-12-15

申请号：US17753922

申请日：2020-09-29

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba

IPC: H01J49/00 ,  H01J49/06

Abstract: Pole electrodes (150) are disclosed for use in an ion reaction apparatus, e.g., an electron induced dissociation cell, to reduce fouling due to polymer build-up and increase the useful lifetime of such electrodes. To reduce fouling, the novel pole electrode designs include a X-shaped aperture (160) in lieu of the conventional central circular aperture. The pole electrodes are particularly useful in systems having a plurality of branched electrodes (152) defining a first axis for controlled passage of charged ions and a transverse axis for passage of an electron beam. The pole electrodes are adapted for disposition between an electron source and the branched electrodes to provide an aperture for passage of an electron beam while also impeding escape of ions and reaction products from the apparatus. The X-shaped aperture eliminates or reduces the portion of the pole electrode surface that is most prone to fouling by polymeric build-up.