公开(公告)号：US10829748B2

公开(公告)日：2020-11-10

申请号：US16305348

申请日：2017-06-08

Applicant: DSM IP Assets B.V.

Inventor： Rene Marcel De Jong ,  Willem Bijleveld ,  Evert Tjeerd Van Rij

IPC: C12N9/20 ,  C12N1/20 ,  C12N1/14 ,  C12N15/52 ,  C12N15/63 ,  A23C13/16 ,  A23C15/12 ,  A23C19/06 ,  A23C19/082

Abstract: The present invention relates to a polypeptide having lipase activity wherein the polypeptide, which, when aligned with a polypeptide according to SEQ ID NO: 1, comprises at least one amino acid substitution resulting in Ser (S), Ala (A) or Leu (L) at position 246, Trp (W) at position 307, Leu (L) at position 345, Ile (I) at position 365, and/or Phe (F) at position 534, wherein the position is defined with reference to SEQ ID NO: 1, wherein Ala(A) at position 1 in SEQ ID NO: 1 is counted as number 1 and a method for preparing the polypeptide.The present invention further relates to a process for preparing a food product wherein a polypeptide according to the present invention is used.

公开(公告)号：US10457890B2

公开(公告)日：2019-10-29

申请号：US15564275

申请日：2016-04-08

Applicant: DSM IP ASSETS B.V.

Inventor： Arjen Sein ,  Rene Marcel De Jong ,  Evert Tjeerd Van Rij ,  Willem Bijleveld ,  Maarten Hotse Wilbrink

IPC: C11B3/00 ,  C12N9/16 ,  C12P7/64 ,  C12P7/20

Abstract: The present invention relates to a polypeptide having phospholipase C activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. A process for degumming a vegetable oil comprising contacting a vegetable oil comprising phospholipids with a polypeptide having phospholipase C activity of the invention or a composition of the invention, wherein phospholipids are hydrolyzed into diacylglycerol and phosphate ester and/or phosphate, separating the phosphate ester and/or phosphate from the vegetable oil wherein a degummed vegetable oil is obtained.

公开(公告)号：US11999923B2

公开(公告)日：2024-06-04

申请号：US17052907

申请日：2019-05-06

Applicant: DSM IP ASSETS B.V.

Inventor： Ying Zha ,  Arjen Sein ,  Willem Bijleveld

IPC: C11B3/00

CPC classification number: C11B3/003 ,  C12Y301/01032

Abstract: The present invention relates to a process for reducing an amount of intact phospholipids in a triacylglyceride oil comprising incubating the oil with a polypeptide having phospholipase A1 activity, wherein the polypeptide comprises a polypeptide having at least 80% identity to the mature amino acid sequence of SEQ ID NO: 1.