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    Screening Method for Inhibitors of Cancer Cell Invasion and Screening System Thereof
    1.
    发明申请
    Screening Method for Inhibitors of Cancer Cell Invasion and Screening System Thereof 审中-公开
    癌细胞侵袭抑制剂筛选方法及筛选方法

    公开(公告)号：US20110104741A1

    公开(公告)日：2011-05-05

    申请号：US13000483

    申请日：2008-12-24

    申请人： Da-Woon Jung ,  Jin Mi Kim ,  Zhong Min Che ,  Young-Tae Chang

    发明人： Da-Woon Jung ,  Jin Mi Kim ,  Zhong Min Che ,  Young-Tae Chang

    IPC分类号： C12Q1/02 ,  C12M1/34

    CPC分类号： G01N33/5011 ,  G01N33/5044

    摘要： This invention relates to a screening method for an inhibitor to cancer cell invasion, comprising the steps of: (a) co-culturing cancer cells and a carcinoma-associated fibroblasts (CAFs) in a multi-chamber containing a upper-chamber, a lower-chamber and a porous filter separating the upper-chamber from the lower-chamber; in which each cancer cells and CAFs is inoculated into the upper-chamber and the lower-chamber of the multi-chamber, and then a candidate is added to the upper-chamber; and (b) measuring the number of cancer cells passing the porous filter. According to the screening system and screening method using the same, the inhibitor to cancer cell invasion is able to be screened in a high-throughput manner.

    摘要翻译： 本发明涉及一种抑制剂对癌细胞侵袭的筛选方法，其特征在于包括以下步骤：（a）在含有上室，下部的多室中共同培养癌细胞和癌相关成纤维细胞（CAF） - 室和将上室与下室分离的多孔过滤器; 其中将每个癌细胞和CAF接种到多室的上室和下室中，然后将候选物加入到上室中; 和（b）测量通过多孔过滤器的癌细胞的数量。 根据使用其的筛选系统和筛选方法，能够以高通量的方式筛选癌细胞侵袭抑制剂。