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20 条记录 (耗时 0.028 秒)

    Concatameric immunoadhesion molecule
    2.
    发明授权
    Concatameric immunoadhesion molecule 有权
    共聚蛋白免疫粘附分子

    公开(公告)号：US07670602B2

    公开(公告)日：2010-03-02

    申请号：US11739288

    申请日：2007-04-24

    申请人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

    发明人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

    IPC分类号： A61K39/395

    CPC分类号： C07K14/70507 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7151 ,  C07K2319/00 ,  C07K2319/30

    摘要： Disclosed are concatameric proteins comprising two soluble domains, in which the C-terminus of a soluble domain of a biologically active protein is linked to the N-terminus of an identical soluble domain or a distinct soluble domain of a biologically active protein. Also, the present invention discloses dimeric proteins formed by formation of intermolecular disulfide bonds at the hinge region of two monomeric proteins formed by linkage of a concatamer of two identical soluble extracellular regions of proteins involving immune response to an Fc fragment of an immunoglobulin molecule, their glycosylated proteins, DNA constructs encoding the monomeric proteins, recombinant expression plasmids containing the DNA construct, host cells transformed or transfected with the recombinant expression plasmids, and a method of preparing the dimeric proteins by culturing the host cells. Further, the present invention discloses pharmaceutical or diagnostic compositions comprising the dimeric protein or its glycosylated form.

    摘要翻译： 公开了包含两个可溶性结构域的连续蛋白质，其中生物活性蛋白质的可溶性结构域的C末端与生物活性蛋白质的相同可溶性结构域或不同可溶性结构域的N末端连接。 此外，本发明公开了通过在两个单体蛋白的铰链区上形成分子间二硫键而形成的二聚体蛋白，该两个单体蛋白是通过将免疫应答涉及免疫应答的两个相同的可溶胞外区的连接体与免疫球蛋白分子的Fc片段 糖基化蛋白质，编码单体蛋白的DNA构建体，含有DNA构建体的重组表达质粒，用重组表达质粒转化或转染的宿主细胞，以及通过培养宿主细胞制备二聚体蛋白质的方法。 此外，本发明公开了包含二聚体蛋白质或其糖基化形式的药物或诊断组合物。

    CONCATAMERIC IMMUNOADHESION MOLECULE
    3.
    发明申请
    CONCATAMERIC IMMUNOADHESION MOLECULE 有权
    综合免疫分子

    公开(公告)号：US20090011466A1

    公开(公告)日：2009-01-08

    申请号：US11739288

    申请日：2007-04-24

    申请人： Yong-Hoon CHUNG ,  Ji-Woong HAN ,  Hye-Ja LEE ,  Eun-Yong CHOI ,  Jin-Mi KIM

    发明人： Yong-Hoon CHUNG ,  Ji-Woong HAN ,  Hye-Ja LEE ,  Eun-Yong CHOI ,  Jin-Mi KIM

    IPC分类号： C12P21/02 ,  C07K14/54 ,  C12N15/62 ,  C12N15/85 ,  C12N5/10

    CPC分类号： C07K14/70507 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7151 ,  C07K2319/00 ,  C07K2319/30

    摘要： Disclosed are concatameric proteins comprising two soluble domains, in which the C-terminus of a soluble domain of a biologically active protein is linked to the N-terminus of an identical soluble domain or a distinct soluble domain of a biologically active protein. Also, the present invention discloses dimeric proteins formed by formation of intermolecular disulfide bonds at the hinge region of two monomeric proteins formed by linkage of a concatamer of two identical soluble extracellular regions of proteins involving immune response to an Fc fragment of an immunoglobulin molecule, their glycosylated proteins, DNA constructs encoding the monomeric proteins, recombinant expression plasmids containing the DNA construct, host cells transformed or transfected with the recombinant expression plasmids, and a method of preparing the dimeric proteins by culturing the host cells. Further, the present invention discloses pharmaceutical or diagnostic compositions comprising the dimeric protein or its glycosylated form.

    摘要翻译： 公开了包含两个可溶性结构域的连续蛋白质，其中生物活性蛋白质的可溶性结构域的C末端与生物活性蛋白质的相同可溶性结构域或不同可溶性结构域的N末端连接。 此外，本发明公开了通过在两个单体蛋白的铰链区上形成分子间二硫键而形成的二聚体蛋白，该两个单体蛋白是通过将免疫应答涉及免疫应答的两个相同的可溶胞外区的连接体与免疫球蛋白分子的Fc片段 糖基化蛋白质，编码单体蛋白的DNA构建体，含有DNA构建体的重组表达质粒，用重组表达质粒转化或转染的宿主细胞，以及通过培养宿主细胞制备二聚体蛋白质的方法。 此外，本发明公开了包含二聚体蛋白质或其糖基化形式的药物或诊断组合物。

    Screening Method for Inhibitors of Cancer Cell Invasion and Screening System Thereof
    7.
    发明申请
    Screening Method for Inhibitors of Cancer Cell Invasion and Screening System Thereof 审中-公开
    癌细胞侵袭抑制剂筛选方法及筛选方法

    公开(公告)号：US20110104741A1

    公开(公告)日：2011-05-05

    申请号：US13000483

    申请日：2008-12-24

    申请人： Da-Woon Jung ,  Jin Mi Kim ,  Zhong Min Che ,  Young-Tae Chang

    发明人： Da-Woon Jung ,  Jin Mi Kim ,  Zhong Min Che ,  Young-Tae Chang

    IPC分类号： C12Q1/02 ,  C12M1/34

    CPC分类号： G01N33/5011 ,  G01N33/5044

    摘要： This invention relates to a screening method for an inhibitor to cancer cell invasion, comprising the steps of: (a) co-culturing cancer cells and a carcinoma-associated fibroblasts (CAFs) in a multi-chamber containing a upper-chamber, a lower-chamber and a porous filter separating the upper-chamber from the lower-chamber; in which each cancer cells and CAFs is inoculated into the upper-chamber and the lower-chamber of the multi-chamber, and then a candidate is added to the upper-chamber; and (b) measuring the number of cancer cells passing the porous filter. According to the screening system and screening method using the same, the inhibitor to cancer cell invasion is able to be screened in a high-throughput manner.

    摘要翻译： 本发明涉及一种抑制剂对癌细胞侵袭的筛选方法，其特征在于包括以下步骤：（a）在含有上室，下部的多室中共同培养癌细胞和癌相关成纤维细胞（CAF） - 室和将上室与下室分离的多孔过滤器; 其中将每个癌细胞和CAF接种到多室的上室和下室中，然后将候选物加入到上室中; 和（b）测量通过多孔过滤器的癌细胞的数量。 根据使用其的筛选系统和筛选方法，能够以高通量的方式筛选癌细胞侵袭抑制剂。