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    Adenovirus vectors specific for cells expressing androgen receptor and methods of use thereof
    5.
    发明授权
    Adenovirus vectors specific for cells expressing androgen receptor and methods of use thereof 失效
    特异于表达雄激素受体的细胞的腺病毒载体及其使用方法

    公开(公告)号：US06197293B1

    公开(公告)日：2001-03-06

    申请号：US09033333

    申请日：1998-03-02

    申请人： Daniel R. Henderson ,  Eric R. Schuur ,  De-Chao Yu

    发明人： Daniel R. Henderson ,  Eric R. Schuur ,  De-Chao Yu

    IPC分类号： A61K4800

    CPC分类号： A61K35/761 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/008

    摘要： Replication-competent adenovirus vectors specific for cells which allows a probasin transcriptional response element (PB-TRE) to function, such as cells which express the androgen receptor (AR), and methods of use of such viruses are provided. These viruses comprise an adenoviral gene under control of a transcriptional regulatory portion of a PB-TRE, which is in turn dependent upon AR expression. The gene can be, for example, a gene required for viral replication or the adenovirus death protein gene (ADP). The viruses can also comprise at least one additional adenoviral gene under control of at least one additional prostate-specific transcriptional response element, such as that controlling prostate-specific antigen expression (PSA-TRE). Thus, virus replication can be restricted to target cells exhibiting prostate-specific gene expression, particularly prostate carcinoma cells. An adenovirus of the present invention can further comprise a heterologous gene such as a reporter under transcriptional control of a PB-TRE. The adenovirus vectors can be used to detect and monitor samples for the presence of prostate cells as well as to selectively kill malignant cells producing prostate-specific gene products.

    摘要翻译： 提供了允许probasin转录应答元件（PB-TRE）起作用的细胞特异性的复制性腺病毒载体，例如表达雄激素受体（AR）的细胞，以及使用这些病毒的方法。 这些病毒包含由PB-TRE的转录调节部分控制的腺病毒基因，其又依赖于AR表达。 该基因可以是例如病毒复制所需的基因或腺病毒死亡蛋白基因（ADP）。 病毒还可以包含至少一种额外的腺病毒基因，其由至少一种另外的前列腺特异性转录应答元件（例如控制前列腺特异性抗原表达（PSA-TRE））控制。 因此，病毒复制可以限于表现出前列腺特异性基因表达的靶细胞，特别是前列腺癌细胞。 本发明的腺病毒可以进一步包含异源基因，例如在PB-TRE的转录控制下的报告物。 腺病毒载体可用于检测和监测样品中前列腺细胞的存在以及选择性杀死产生前列腺特异性基因产物的恶性细胞。

    Human glandular kallikrein enhancer, vectors comprising the enhancer and methods of use thereof
    7.
    发明授权
    Human glandular kallikrein enhancer, vectors comprising the enhancer and methods of use thereof 失效
    人腺激肽释放酶增强剂，包含增强子的载体及其使用方法

    公开(公告)号：US06916918B2

    公开(公告)日：2005-07-12

    申请号：US09875228

    申请日：2001-06-05

    申请人： De Chao Yu ,  Daniel R. Henderson ,  Eric R. Schuur

    发明人： De Chao Yu ,  Daniel R. Henderson ,  Eric R. Schuur

    IPC分类号： A61K48/00 ,  C07K14/075 ,  C12N15/57 ,  C12N15/861 ,  C07H21/04 ,  C12N15/74

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2830/002 ,  C12N2830/008

    摘要： Enhancers which preferentially increase the transcription of cis-linked coding sequences in prostate cells are provided. Methods of using DNA constructs comprising the enhancers to control transcription of heterologous polynucleotides are also provided. Delivery vehicles comprising the enhancers and methods of using the vehicles are also provided. Adenovirus vectors in which one or more genes are under transcriptional control of the enhancers of the invention are also provided. Further provided are methods of using the adenovirus vectors of the invention to confer selective cytotoxicity in mammalian cells.

    摘要翻译： 提供优先增加前列腺细胞中顺式连接编码序列的转录的增强子。 还提供了使用包含增强子的DNA构建体来控制异源多核苷酸转录的方法。 还提供了包含增强剂和使用车辆的方法的输送车辆。 还提供了其中一个或多个基因在本发明的增强子的转录控制下的腺病毒载体。 还提供了使用本发明的腺病毒载体在哺乳动物细胞中赋予选择性细胞毒性的方法。