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1.发明授权Cellulase variants with improved expression, activity and stability, and use thereof 有权具有改善的表达，活性和稳定性的纤维素酶变体及其用途公开(公告)号：US09523087B2
公开(公告)日：2016-12-20
申请号：US14189943
申请日：2014-02-25
Applicant: Danisco US Inc.
Inventor： Wolfgang Aehle , Richard R. Bott , Benjamin S. Bower , Jonathan Caspi , David A. Estell , Frits Goedegebuur , Ronaldus Wilhelmus Joannes Hommes , Thijs Kaper , Bradley R. Kelemen , Slavko Kralj , Johannes Franciscus Thomas Van Lieshout , Igor Nikolaev , Sander Van Stigt Thans , Louise Wallace , Gudrun Vogtentanz , Mats Sandgren
CPC classification number: C12N9/2437 , C12Y302/01091 , Y02E50/17
Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.
Abstract translation: 本公开涉及纤维素酶变体。 特别地，本公开涉及具有改善的表达，活性和/或稳定性的纤维素酶变体。 还描述了编码纤维素酶变体的核酸，包含纤维素酶变体的组合物及其使用方法。
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公开(公告)号：US09334467B2
公开(公告)日：2016-05-10
申请号：US14037883
申请日：2013-09-26
Applicant: Danisco US Inc.
Inventor： Andrew Shaw , Louise Wallace , David A. Estell , Ronaldus W. J. Hommes , Sang-Kyu Lee , Hiroshi Oh , Eugene S. Sadlowski
IPC: C12N9/54 , C12N9/50 , C12P21/06 , C12P19/34 , C12N15/00 , C12N1/20 , C11D3/00 , C07H21/04 , C07K1/00 , C11D3/386
CPC classification number: C12N9/54 , C11D1/02 , C11D3/386 , C11D3/38663 , C12Y304/24
Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
Abstract translation: 本发明提供包含至少一种具有改善的储存稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中，中性金属蛋白酶可用于清洁和其它应用。 在一些特别优选的实施方案中，本发明提供了包含从芽孢杆菌属芽孢杆菌获得的中性金属蛋白酶的方法和组合物。 在一些更特别优选的实施方案中，中性金属蛋白酶从解淀粉芽孢杆菌获得。 在另外的优选实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的变体。 在另外的实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的同系物。 本发明特别用于包括但不限于清洁，漂白和消毒的应用。
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公开(公告)号：US20200231908A1
公开(公告)日：2020-07-23
申请号：US16798761
申请日：2020-02-24
Applicant: DANISCO US INC.
Inventor： RONALDUS W. J. HOMMES , Louise Wallace , Andrew Shaw , Amy D. Liu
Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved stability in the presence of a metal chelator. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications comprising citrate. In some particularly preferred embodiments, the present invention provides methods and compositions comprising variant neutral metalloprotease(s) engineered to resist citrate-induced autolysis.
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公开(公告)号：US12043853B2
公开(公告)日：2024-07-23
申请号：US17376669
申请日：2021-07-15
Applicant: DANISCO US INC
Inventor： Andrew Shaw , David A Estell , Eugene S Sadlowski , Hiroshi Oh , Louise Wallace , Ronaldus Wilhelmus Joannes Hommes , Sang-Kyu Lee
CPC classification number: C12N9/54 , C11D1/02 , C11D3/386 , C11D3/38663 , C12Y304/24
Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
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公开(公告)号：US11091750B2
公开(公告)日：2021-08-17
申请号：US16668635
申请日：2019-10-30
Applicant: DANISCO US INC
Inventor： Andrew Shaw , David A Estell , Eugene S Sadlowski , Hiroshi Oh , Louise Wallace , Ronaldus Wilhelmus Joannes Hommes , Sang-Kyu Lee
Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
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Patent Agency Ranking
公开(公告)号：US20170022490A1
公开(公告)日：2017-01-26
申请号：US15094761
申请日：2016-04-08
Applicant: Danisco US Inc.
Inventor： Andrew Shaw , Louise Wallace , David A. Estell , Ronaldus W.J. Hommes , Sang-Kyu Lee , Hiroshi Oh , Eugene S. Sadlowski
CPC classification number: C12N9/54 , C11D1/02 , C11D3/386 , C11D3/38663 , C12Y304/24
Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
Abstract translation: 本发明提供包含至少一种具有改善的储存稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中，中性金属蛋白酶可用于清洁和其它应用。 在一些特别优选的实施方案中，本发明提供了包含从芽孢杆菌属芽孢杆菌获得的中性金属蛋白酶的方法和组合物。 在一些更特别优选的实施方案中，中性金属蛋白酶从解淀粉芽孢杆菌获得。 在另外的优选实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的变体。 在另外的实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的同系物。 本发明特别用于包括但不限于清洁，漂白和消毒的应用。
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公开(公告)号：US20140134708A1
公开(公告)日：2014-05-15
申请号：US14037883
申请日：2013-09-26
Applicant: Danisco US Inc.
Inventor： Andrew Shaw , Louise Wallace , David A. Estell , Ronaldus W.J. Hommes , Sang-Kyu Lee , Hiroshi Oh , Eugene S. Sadlowski
CPC classification number: C12N9/54 , C11D1/02 , C11D3/386 , C11D3/38663 , C12Y304/24
Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
Abstract translation: 本发明提供包含至少一种具有改善的储存稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中，中性金属蛋白酶可用于清洁和其它应用。 在一些特别优选的实施方案中，本发明提供了包含从芽孢杆菌属芽孢杆菌获得的中性金属蛋白酶的方法和组合物。 在一些更特别优选的实施方案中，中性金属蛋白酶从解淀粉芽孢杆菌获得。 在另外的优选实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的变体。 在另外的实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的同系物。 本发明特别用于包括但不限于清洁，漂白和消毒的应用。
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公开(公告)号：US10577595B2
公开(公告)日：2020-03-03
申请号：US15788079
申请日：2017-10-19
Applicant: DANISCO US INC
Inventor： Andrew Shaw , David A Estell , Eugene S Sadlowski , Hiroshi Oh , Louise Wallace , Ronaldus Wilhelmus Joannes Hommes , Sang-Kyu Lee
Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
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公开(公告)号：US20180094216A1
公开(公告)日：2018-04-05
申请号：US15834446
申请日：2017-12-07
Applicant: DANISCO US INC.
Inventor： Ronaldus W.J. Hommes , Louise Wallace , Andrew Shaw , Amy D. Liu
CPC classification number: C11D3/38618 , C12N9/54
Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved stability in the presence of a metal chelator. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications comprising citrate. In some particularly preferred embodiments, the present invention provides methods and compositions comprising variant neutral metalloprotease(s) engineered to resist citrate-induced autolysis.
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10.发明申请VARIANT ALPHA-AMYLASES FROM BACILLUS SUBTILIS AND METHODS OF USE, THEREOF 审中-公开来自BACILLUS SUBTILIS的变体ALPHA-淀粉及其使用方法公开(公告)号：US20160010129A1
公开(公告)日：2016-01-14
申请号：US14742519
申请日：2015-06-17
Applicant: Danisco US Inc.
Inventor： William A. Cuevas , Sang-Kyu Lee , Sandra W. Ramer , Andrew Shaw , Amr R. Toppozada , David A. Estell , Louise Wallace , Regina Chin , Carol A. Requadt , Scott D. Power , Michael J. Pepsin
Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
Abstract translation: 提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。
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