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    Methods of increasing polypeptide accumulation in plants
    1.
    发明授权
    Methods of increasing polypeptide accumulation in plants 失效
    增加植物多肽积累的方法

    公开(公告)号：US06855871B2

    公开(公告)日：2005-02-15

    申请号：US09934066

    申请日：2001-08-21

    申请人： Darren B. Gruis ,  Rudolf Jung

    发明人： Darren B. Gruis ,  Rudolf Jung

    IPC分类号： C07K16/00 ,  C12N9/50 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/06 ,  A01H5/10

    CPC分类号： C07K16/00 ,  C07K2317/13 ,  C07K2317/622 ,  C12N9/50 ,  C12N15/8251 ,  C12N15/8257 ,  C12N15/8258

    摘要： The present invention provides methods and compositions directed to the production of polypeptides in plant protein storage tissue. Methods of the invention include methods for producing polypeptides in protein storage tissues by decreasing the level of protease activity in the plant protein storage tissue. Compositions of the invention include plants that are genetically modified such that the activity of one or more proteases is reduced or eliminated, transformed seeds obtained from these genetically modified plants, and sequences encoding a novel member of the VPE cysteine protease family found in seed, ε-VPE. These methods and compositions find utility in altering the nutritional or other qualities of seed, and in producing useful proteins including pharmacological or industrial proteins.

    摘要翻译： 本发明提供了用于在植物蛋白质储存组织中产生多肽的方法和组合物。 本发明的方法包括通过降低植物蛋白质储存组织中蛋白酶活性的水平来在蛋白质储存组织中产生多肽的方法。 本发明的组合物包括被遗传修饰的植物，使得一种或多种蛋白酶的活性被降低或消除，从这些遗传修饰的植物获得的转化的种子，以及编码在种子中的VPE半胱氨酸蛋白酶家族的新成员的序列， -VPE。 这些方法和组合物可用于改变种子的营养或其他品质，以及生产有用的蛋白质，包括药理学或工业蛋白质。

    Methods of increasing polypeptide accumulation in plants
    3.
    发明授权
    Methods of increasing polypeptide accumulation in plants 失效
    增加植物多肽积累的方法

    公开(公告)号：US07557263B2

    公开(公告)日：2009-07-07

    申请号：US11971708

    申请日：2008-01-09

    申请人： Darren B. Gruis ,  Rudolf Jung

    发明人： Darren B. Gruis ,  Rudolf Jung

    IPC分类号： C12N15/82 ,  C12N15/87 ,  C01H21/20 ,  A01H5/00

    CPC分类号： C07K16/00 ,  C07K2317/13 ,  C07K2317/622 ,  C12N9/50 ,  C12N15/8251 ,  C12N15/8257 ,  C12N15/8258

    摘要： The present invention provides methods and compositions directed to the production of polypeptides in plant protein storage tissue. Methods of the invention include methods for producing polypeptides in protein storage tissues by decreasing the level of protease activity in the plant protein storage tissue. Compositions of the invention include plants that are genetically modified such that the activity of one or more proteases is reduced or eliminated, transformed seeds obtained from these genetically modified plants, and sequences encoding a novel member of the VPE cysteine protease family found in seed, ε-VPE. These methods and compositions find utility in altering the nutritional or other qualities of seed, and in producing useful proteins including pharmacological or industrial proteins.

    摘要翻译： 本发明提供了用于在植物蛋白质储存组织中产生多肽的方法和组合物。 本发明的方法包括通过降低植物蛋白质储存组织中蛋白酶活性的水平来在蛋白质储存组织中产生多肽的方法。 本发明的组合物包括被遗传修饰的植物，使得一种或多种蛋白酶的活性被降低或消除，从这些遗传修饰的植物获得的转化的种子，以及编码在种子中的VPE半胱氨酸蛋白酶家族的新成员的序列， -VPE。 这些方法和组合物可用于改变种子的营养或其他品质，以及生产有用的蛋白质，包括药理学或工业蛋白质。

    METHODS OF INCREASING POLYPEPTIDE ACCUMULATION IN PLANTS
    4.
    发明申请
    METHODS OF INCREASING POLYPEPTIDE ACCUMULATION IN PLANTS 失效
    增加植物多糖积累的方法

    公开(公告)号：US20080124804A1

    公开(公告)日：2008-05-29

    申请号：US11971708

    申请日：2008-01-09

    申请人： Darren B. Gruis ,  Rudolf Jung

    发明人： Darren B. Gruis ,  Rudolf Jung

    IPC分类号： C12N15/82

    CPC分类号： C07K16/00 ,  C07K2317/13 ,  C07K2317/622 ,  C12N9/50 ,  C12N15/8251 ,  C12N15/8257 ,  C12N15/8258

    摘要： The present invention provides methods and compositions directed to the production of polypeptides in plant protein storage tissue. Methods of the invention include methods for producing polypeptides in protein storage tissues by decreasing the level of protease activity in the plant protein storage tissue. Compositions of the invention include plants that are genetically modified such that the activity of one or more proteases is reduced or eliminated, transformed seeds obtained from these genetically modified plants, and sequences encoding a novel member of the VPE cysteine protease family found in seed, ε-VPE. These methods and compositions find utility in altering the nutritional or other qualities of seed, and in producing useful proteins including pharmacological or industrial proteins.

    摘要翻译： 本发明提供了用于在植物蛋白质储存组织中产生多肽的方法和组合物。 本发明的方法包括通过降低植物蛋白质储存组织中蛋白酶活性的水平来在蛋白质储存组织中产生多肽的方法。 本发明的组合物包括被遗传修饰的植物，使得一种或多种蛋白酶的活性被降低或消除，从这些遗传修饰的植物获得的转化的种子，以及编码在种子中的VPE半胱氨酸蛋白酶家族的新成员的序列， -VPE。 这些方法和组合物可用于改变种子的营养或其他品质，以及生产有用的蛋白质，包括药理学或工业蛋白质。

    Methods and compositions for in planta production of inverted repeats
    5.
    发明授权
    Methods and compositions for in planta production of inverted repeats 失效
    在反向重复的植物生产中的方法和组合物

    公开(公告)号：US08058509B2

    公开(公告)日：2011-11-15

    申请号：US11611661

    申请日：2006-12-15

    申请人： Kimberly Glassman ,  William J. Gordon-Kamm ,  Rudolf Jung

    发明人： Kimberly Glassman ,  William J. Gordon-Kamm ,  Rudolf Jung

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N15/87

    CPC分类号： C12N15/8213 ,  C12N15/8216

    摘要： Methods and compositions are provided which allow for the production of an inverted repeat in a plant or plant part, which, when transcribed as an RNA can be used in some examples to decrease expression of a target polynucleotide of interest. The methods and composition provide precursor inverted repeat cassettes that are not capable of producing a hairpin RNA polynucleotide, and plants and plant parts comprising such precursor inverted repeat cassettes. The precursor inverted repeat cassettes are altered in planta by using a recombination system to produce a inverted repeat. Plants, plant parts, and seeds comprising the various components are also provided.

    摘要翻译： 提供了允许在植物或植物部分中产生反向重复的方法和组合物，其在转录为RNA时可用于一些实例中以减少目的目的多核苷酸的表达。 方法和组合物提供不能产生发夹RNA多核苷酸的前体反向重复盒，以及包含这种前体反向重复盒的植物和植物部分。 通过使用重组系统产生反向重复，在植物中改变前体反向重复盒。 还提供了包括各种组分的植物，植物部分和种子。

    Seed-Preferred Promoters
    6.
    发明申请
    Seed-Preferred Promoters 有权
    种子优选的启动子

    公开(公告)号：US20090089897A1

    公开(公告)日：2009-04-02

    申请号：US12236822

    申请日：2008-09-24

    申请人： Shane E. Abbitt ,  Rudolf Jung ,  David A. Selinger

    发明人： Shane E. Abbitt ,  Rudolf Jung ,  David A. Selinger

    IPC分类号： C12N15/82 ,  C07H21/04 ,  A01H5/00

    CPC分类号： C12N15/8234

    摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the sorghum δ-kafirin coding region. The sequences drive expression preferentially to endosperm tissue. A method for expressing a nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

    摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物是从高粱delta-kafirin编码区分离的组织优选启动子的新核苷酸序列。 序列优先驱动表达胚乳组织。 提供了使用本文公开的调节序列在植物中表达核苷酸序列的方法。 该方法包括转化植物细胞以包含可操作地连接到本发明的一种或多种调节序列的核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

    Seed-preferred regulatory elements
    7.
    发明授权
    Seed-preferred regulatory elements 有权
    种子优先的监管要素

    公开(公告)号：US07211712B2

    公开(公告)日：2007-05-01

    申请号：US11284201

    申请日：2005-11-21

    申请人： Shane E. Abbitt ,  Rudolf Jung

    发明人： Shane E. Abbitt ,  Rudolf Jung

    IPC分类号： C12P7/62 ,  C12P11/00 ,  C07H21/04 ,  A01H1/00

    CPC分类号： C12N15/8234

    摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a seed-preferred promoter and terminator isolated from the maize legumin 1 coding region. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

    摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物是从玉米豆科1编码区分离的种子优选启动子和终止子的新核苷酸序列。 提供了使用本文公开的调节序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化为包含可操作地连接到本发明的一个或多个调控序列的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

    Manufacture of high impact and translucent styrene polymers
    9.
    发明授权
    Manufacture of high impact and translucent styrene polymers 失效
    高冲击和半透明苯乙烯聚合物的制造

    公开(公告)号：US4282334A

    公开(公告)日：1981-08-04

    申请号：US97815

    申请日：1979-11-27

    申请人： Manfred Walter ,  Dieter Stein ,  Gerhard Fahrbach ,  Rudolf Jung ,  Adolf Echte

    发明人： Manfred Walter ,  Dieter Stein ,  Gerhard Fahrbach ,  Rudolf Jung ,  Adolf Echte

    IPC分类号： C08F291/00 ,  C08F279/02 ,  C08F291/02

    CPC分类号： C08F279/02

    摘要： A process for the manufacture of high impact and translucent polystyrene, in which, in a first stage, styrene is prepolymerized in mass or in solution in the presence of a rubber, and the polymerization is then completed in mass, in solution or in aqueous suspension. The prepolymerization is taken to a stage where the amount of styrene converted is from 3 to 10 times the amount of the elastomer constituent of the rubber, and at this conversion the disperse soft-component phase formed should have a mean particle size of less than 1 .mu.m and should contain from 35 to 65% by weight of free or chemically bonded polystyrene segments. The translucent styrene polymers of high impact strength may be used for packaging purposes.

    摘要翻译： 用于制造高冲击和半透明聚苯乙烯的方法，其中在第一阶段中，苯乙烯在橡胶存在下以质量或溶液预聚合，然后在溶液或水悬浮液中完成聚合 。 将预聚合物进行到苯乙烯转化量为橡胶的弹性体成分的3〜10倍的阶段，在该转化条件下，形成的分散软组分相的平均粒径应小于1 并且应含有35至65重量％的游离或化学键合的聚苯乙烯段。 具有高冲击强度的半透明苯乙烯聚合物可用于包装目的。