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1.发明授权Device and methods for detecting the response of a plurality of cells to at least one analyte of interest 有权用于检测多个细胞对至少一种感兴趣分析物的响应的装置和方法公开(公告)号：US07713733B2
公开(公告)日：2010-05-11
申请号：US10483466
申请日：2002-08-06
申请人： David Cliffel , Franz J. Baudenbacher , John P. Wikswo , Sven Eklund , R. Robert Balcarcel , Jonathan M. Gilligan
发明人： David Cliffel , Franz J. Baudenbacher , John P. Wikswo , Sven Eklund , R. Robert Balcarcel , Jonathan M. Gilligan
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： An apparatus and methods for detecting at least one analyte of interest either produced or consumed by a plurality of cell. In one embodiment of the present invention, the method includes the steps of providing a housing defining a chamber, placing a plurality of cells in the chamber, and simultaneously detecting at least two analytes of interest either produced or consumed by the plurality of cells in the chamber.
摘要翻译： 一种用于检测由多个细胞产生或消耗的至少一种感兴趣分析物的装置和方法。 在本发明的一个实施例中，该方法包括以下步骤：提供限定腔室的壳体，将多个细胞放置在腔室中，同时检测至少两种感兴趣的分析物，所述分析物是由多个细胞产生或消耗的 房间。
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2.发明申请Apparatus and methods for monitoring the status of a metabolically active cell 有权用于监测代谢活性细胞状态的装置和方法公开(公告)号：US20090305319A1
公开(公告)日：2009-12-10
申请号：US10213049
申请日：2002-08-06
申请人： Franz J. Baudenbacher , John P. Wikswo , R. Robert Balcarcel , David Cliffel , Sven Eklund , Jonathan Mark Gilligan , Owen McGuinness , Todd Monroe , Ales Prokop , Mark Andrew Stremler , Andreas Augustinus Werdich
发明人： Franz J. Baudenbacher , John P. Wikswo , R. Robert Balcarcel , David Cliffel , Sven Eklund , Jonathan Mark Gilligan , Owen McGuinness , Todd Monroe , Ales Prokop , Mark Andrew Stremler , Andreas Augustinus Werdich
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： An apparatus and methods for monitoring the status of a cell that consumes oxygen. In one embodiment of the present invention, the method includes the steps of confining the cell in a sensing volume, measuring dynamically intracellular or extracellular signaling of the cell, and determining the status of the cell from the measured intracellular or extracellular signaling of the cell.
摘要翻译： 用于监测消耗氧气的电池的状态的装置和方法。 在本发明的一个实施方案中，该方法包括以下步骤：将细胞限制在感测体积中，动态地测量细胞内的细胞内或细胞外信号传导，以及根据测定的细胞内或细胞外信号传导确定细胞的状态。
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3.发明申请公开(公告)号：US20120156763A1
公开(公告)日：2012-06-21
申请号：US13218287
申请日：2011-08-25
申请人： John P. Wikswo , Franz J. Baudenbacher , R. Robert Balcarcel , Theodore A. Bapty , David Cliffel , Sven Eklund , Owen McGuinness , Todd Monroe , Ales Prokop , Mark Andrew Stremler , Andreas Augustinus Werdich , Yang Yuansheng
发明人： John P. Wikswo , Franz J. Baudenbacher , R. Robert Balcarcel , Theodore A. Bapty , David Cliffel , Sven Eklund , Owen McGuinness , Todd Monroe , Ales Prokop , Mark Andrew Stremler , Andreas Augustinus Werdich , Yang Yuansheng
IPC分类号： C12M1/34
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： An apparatus and methods for using biological material to discriminate an agent. In one embodiment of the present invention, the method includes the steps of providing at least one cell, exposing at least one cell to an agent, measuring the response of the cell to the agent in terms of a physical quantity related to at least one of the cellular physiological activities of the cell, and identifying the agent from the measured response. The method further includes the step of quantifying the agent from the measured response.
摘要翻译： 用于使用生物材料来鉴别试剂的装置和方法。 在本发明的一个实施方案中，该方法包括以下步骤：提供至少一个细胞，将至少一个细胞暴露于药剂，根据与下列中的至少一个相关的物理量测量细胞对药剂的反应： 细胞的细胞生理活性，以及根据测定的反应鉴定药剂。 该方法还包括从所测量的响应量化试剂的步骤。
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4.发明授权Apparatus and methods for monitoring the status of a metabolically active cell 有权用于监测代谢活性细胞状态的装置和方法公开(公告)号：US07704745B2
公开(公告)日：2010-04-27
申请号：US10213049
申请日：2002-08-06
申请人： Franz Baudenbacher , John P. Wikswo , R. Robert Balcarcel , David Cliffel , Sven Eklund , Jonathan Mark Gilligan , Owen McGuinness , Todd Monroe , Ales Prokop , Mark Andrew Stremler , Andreas Augustinus Werdich
发明人： Franz Baudenbacher , John P. Wikswo , R. Robert Balcarcel , David Cliffel , Sven Eklund , Jonathan Mark Gilligan , Owen McGuinness , Todd Monroe , Ales Prokop , Mark Andrew Stremler , Andreas Augustinus Werdich
IPC分类号： G01N33/00
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： An apparatus and methods for monitoring the status of a cell that consumes oxygen. In one embodiment of the present invention, the method includes the steps of confining the cell in a sensing volume, measuring dynamically intracellular or extracellular signaling of the cell, and determining the status of the cell from the measured intracellular or extracellular signaling of the cell.
摘要翻译： 用于监测消耗氧气的电池的状态的装置和方法。 在本发明的一个实施方案中，该方法包括以下步骤：将细胞限制在感测体积中，动态地测量细胞内的细胞内或细胞外信号传导，以及根据测量的细胞内或细胞外信号传导确定细胞的状态。
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公开(公告)号：US20090215654A1
公开(公告)日：2009-08-27
申请号：US12398825
申请日：2009-03-05
申请人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面由第二基板的第二表面接收以覆盖空腔，以形成用于接收电池和液体介质的通道。 在形成生物反应器时，通道的大小适于允许生物相容性涂层上的细胞层的生长和通道中的液体流。 控制液体的流动以便向细胞层提供已知的剪切力。 可以进一步控制液体的流动，以便提供模拟通道中血管空间的环境。
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公开(公告)号：US20120003729A1
公开(公告)日：2012-01-05
申请号：US13175659
申请日：2011-07-01
申请人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面由第二基板的第二表面接收以覆盖空腔，以形成用于接收电池和液体介质的通道。 在形成生物反应器时，通道的大小适于允许生物相容性涂层上的细胞层的生长和通道中的液体流。 控制液体的流动以便向细胞层提供已知的剪切力。 可以进一步控制液体的流动，以便提供模拟通道中血管空间的环境。
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公开(公告)号：US07534601B2
公开(公告)日：2009-05-19
申请号：US10525559
申请日：2003-08-27
申请人： John P. Wikswo , Franz J. Baudenbacher , Alex Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , Alex Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
IPC分类号： C12M1/00
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面由第二基板的第二表面接收以覆盖空腔，以形成用于接收电池和液体介质的通道。 在形成生物反应器时，通道的大小适于允许生物相容性涂层上的细胞层的生长和通道中的液体流。 控制液体的流动以便向细胞层提供已知的剪切力。 可以进一步控制液体的流动，以便提供模拟通道中血管空间的环境。
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公开(公告)号：US20110294202A1
公开(公告)日：2011-12-01
申请号：US13116174
申请日：2011-05-26
申请人： John P. Wikswo , Franz J. Baudenbacher , David Cliffel , Frederick R. Haselton , Eugene J. Leboeuf , Ales Prokop , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , David Cliffel , Frederick R. Haselton , Eugene J. Leboeuf , Ales Prokop , Randall S. Reiserer , Mark A. Stremler
IPC分类号： C12M1/34
CPC分类号： C12M23/16 , B01L3/5027 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/163 , B01L2400/0472 , C12M23/12 , C12M29/10 , C12M41/00 , Y10S435/808
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving the cells and the liquid medium. The bioreactor further has a barrier dividing the chamber into a first subchamber and a second subchamber, wherein the barrier has a porosity to allow the first subchamber and the second subchamber in fluid communication and allow at least one predetermined type of cells to permeate between the first subchamber and the second subchamber.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器具有第一基底，其具有第一表面和相对的第二表面，在其间限定用于接收细胞和液体介质的腔室。 所述生物反应器还具有将所述室分成第一子室和第二子室的屏障，其中所述阻挡层具有孔隙率以允许所述第一子室和所述第二子室流体连通，并允许至少一个预定类型的泡孔在所述第一室 子房和第二个子房。
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公开(公告)号：US07977089B2
公开(公告)日：2011-07-12
申请号：US10525549
申请日：2003-08-27
申请人： John P. Wikswo , Franz J. Baudenbacher , David Cliffel , Frederick R. Haselton , Eugene J. Leboeuf , Ales Prokop , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , David Cliffel , Frederick R. Haselton , Eugene J. Leboeuf , Ales Prokop , Randall S. Reiserer , Mark A. Stremler
CPC分类号： C12M23/16 , B01L3/5027 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/163 , B01L2400/0472 , C12M23/12 , C12M29/10 , C12M41/00 , Y10S435/808
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving the cells and the liquid medium. The bioreactor further has a barrier dividing the chamber into a first subchamber and a second subchamber, wherein the barrier has a porosity to allow the first subchamber and the second subchamber in fluid communication and allow at least one predetermined type of cells to permeate between the first subchamber and the second subchamber.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器具有第一基底，其具有第一表面和相对的第二表面，在其间限定用于接收细胞和液体介质的腔室。 所述生物反应器还具有将所述室分成第一子室和第二子室的屏障，其中所述阻挡层具有孔隙率以允许所述第一子室和所述第二子室流体连通，并允许至少一个预定类型的泡孔在所述第一室 子房和第二个子房。
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10.发明申请公开(公告)号：US20060073539A1
公开(公告)日：2006-04-06
申请号：US10212918
申请日：2002-08-06
申请人： John Wikswo , Franz Baudenbacher , R. Balcarcel , Theodore Bapty , David Cliffel , Sven Eklund , Owen McGuinness , Todd Monroe , Ales Prokop , Mark Stremler , Andreas Werdich , Yang Yuansheng
发明人： John Wikswo , Franz Baudenbacher , R. Balcarcel , Theodore Bapty , David Cliffel , Sven Eklund , Owen McGuinness , Todd Monroe , Ales Prokop , Mark Stremler , Andreas Werdich , Yang Yuansheng
IPC分类号： C12Q1/02
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： An apparatus and methods for using biological material to discriminate an agent. In one embodiment of the present invention, the method includes the steps of providing at least one cell, exposing at least one cell to an agent, measuring the response of the cell to the agent in terms of a physical quantity related to at least one of the cellular physiological activities of the cell, and identifying the agent from the measured response. The method further includes the step of quantifying the agent from the measured response.
摘要翻译： 用于使用生物材料来鉴别试剂的装置和方法。 在本发明的一个实施方案中，该方法包括以下步骤：提供至少一个细胞，将至少一个细胞暴露于药剂，根据与下列中的至少一个相关的物理量测量细胞对药剂的反应： 细胞的细胞生理活性，以及根据测定的反应鉴定药剂。 该方法还包括从所测量的响应量化试剂的步骤。
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