公开(公告)号：US06709810B2

公开(公告)日：2004-03-23

申请号：US09912031

申请日：2001-07-23

Applicant: David Cook ,  Adonis Stassinopoulos

Inventor： David Cook ,  Adonis Stassinopoulos

IPC: A01N102

CPC classification number: A61L2/0088

Abstract: Methods are provided for quenching undesired side reactions of pathogen inactivating compounds in biological materials. In a particular embodiment, methods are provided for quenching undesired side reactions of a pathogen inactivating compound that includes a functional group which is, or which is capable of forming, an electrophilic group. In this embodiment, the material is treated with the pathogen inactivating compound and a quencher, wherein the quencher comprises a nucleophilic functional group that is capable of covalently reacting with the electrophilic group. The electrophilic group on the pathogen inactivating compound is preferably a non-radical cationic group. In one embodiment, the pathogen inactivating compound includes a nucleic acid binding ligand and a mustard group, wherein the mustard group is capable of reacting in situ to form the electrophilic group. Preferred quenchers are thiols, such as glutathione. Biological materials which may be treated include whole blood, red blood cells, blood plasma, and platelets. The methods permit inhibition of the modification of red blood cells in red blood cell containing materials during pathogen inactivation.

Abstract translation: 提供了用于猝灭病原体灭活化合物在生物材料中的不希望的副反应的方法。 在一个具体实施方案中，提供了用于猝灭病原体灭活化合物的不希望的副反应的方法，其包括可亲电子基团或能够形成亲电基团的官能团。 在该实施方案中，用病原体灭活化合物和猝灭剂处理材料，其中猝灭剂包含能够与亲电子基团共价反应的亲核官能团。 病原体灭活化合物上的亲电基团优选为非自由基阳离子基团。 在一个实施方案中，病原体灭活化合物包括核酸结合配体和芥子基团，其中芥末基团能够在原位反应形成亲电子基团。 优选的猝灭剂是硫醇，如谷胱甘肽。 可以治疗的生物材料包括全血，红细胞，血浆和血小板。 该方法允许在病原体灭活期间抑制含红细胞的材料中的红细胞的修饰。

公开(公告)号：US06270952B1

公开(公告)日：2001-08-07

申请号：US09110776

申请日：1998-07-06

Applicant: David Cook ,  Adonis Stassinopoulos

Inventor： David Cook ,  Adonis Stassinopoulos

IPC: A01N102

CPC classification number: A61L2/0088

Abstract: Methods are provided for quenching undesired side reactions of pathogen inactivating compounds in biological materials. In a particular embodiment, methods are provided for quenching undesired side reactions of a pathogen inactivating compound that includes a functional group which is, or which is capable of forming, an electrophilic group. In this embodiment, the material is treated with the pathogen inactivating compound and a quencher, wherein the quencher comprises a nucleophilic functional group that is capable of covalently reacting with the electrophilic group. The electrophilic group on the pathogen inactivating compound is preferably a non-radical cationic group. In one embodiment, the pathogen inactivating compound includes a nucleic acid binding ligand and a mustard group, wherein the mustard group is capable of reacting in situ to form the electrophilic group. Preferred quenchers are thiols, such as glutathione. Biological materials which may be treated include whole blood, red blood cells, blood plasma, and platelets. The methods permit inhibition of the modification of red blood cells in red blood cell containing materials during pathogen inactivation.

Abstract translation: 提供了用于猝灭病原体灭活化合物在生物材料中的不希望的副反应的方法。 在一个具体实施方案中，提供了用于猝灭病原体灭活化合物的不希望的副反应的方法，其包括可亲电子基团或能够形成亲电基团的官能团。 在该实施方案中，用病原体灭活化合物和猝灭剂处理材料，其中猝灭剂包含能够与亲电子基团共价反应的亲核官能团。 病原体灭活化合物上的亲电基团优选为非自由基阳离子基团。 在一个实施方案中，病原体灭活化合物包括核酸结合配体和芥子基团，其中芥末基团能够在原位反应形成亲电子基团。 优选的猝灭剂是硫醇，如谷胱甘肽。 可以治疗的生物材料包括全血，红细胞，血浆和血小板。 该方法允许在病原体灭活期间抑制含红细胞的材料中的红细胞的修饰。

公开(公告)号：US6093725A

公开(公告)日：2000-07-25

申请号：US3115

申请日：1998-01-06

Applicant: David Cook ,  John E. Merritt ,  Aileen Nerio ,  Henry Rapoport ,  Adonis Stassinopoulos ,  Susan Wollowitz ,  Jan Matejovic

Inventor： David Cook ,  John E. Merritt ,  Aileen Nerio ,  Henry Rapoport ,  Adonis Stassinopoulos ,  Susan Wollowitz ,  Jan Matejovic

IPC: A61L2/00 ,  A61M1/02 ,  B01J20/28 ,  C07D219/10 ,  C07D405/12 ,  C07D493/04 ,  C07F9/64 ,  A61K31/44 ,  C07D219/08

CPC classification number: C07D405/12 ,  A61L2/0082 ,  A61L2/0088 ,  A61M1/0209 ,  B01J20/28 ,  C07D219/10 ,  C07D493/04 ,  C07F9/64

Abstract: Compounds and methods for inactivating pathogens in materials are described, including compositions and methods for inactivating pathogens in biological materials such as red blood cell preparations and plasma. The compounds and methods may be used to treat materials intended for in vitro or in vivo use, such as clinical testing or transfusion. The compounds are designed to specifically bind to and react with nucleic acid, and then to degrade to form breakdown products. The degradation reaction is preferably slower than the reaction with nucleic acid.

公开(公告)号：US06514987B1

公开(公告)日：2003-02-04

申请号：US09610652

申请日：2000-07-02

Applicant: David Cook ,  John E. Merritt ,  Aileen Nerio ,  Henry Rapoport ,  Adonis Stassinopoulos ,  Susan Wollowitz ,  Jan Matejovic ,  William A. Denny

Inventor： David Cook ,  John E. Merritt ,  Aileen Nerio ,  Henry Rapoport ,  Adonis Stassinopoulos ,  Susan Wollowitz ,  Jan Matejovic ,  William A. Denny

IPC: A61K3144

CPC classification number: C07D405/12 ,  A61L2/0082 ,  A61M1/0209 ,  B01J20/28 ,  C07D219/10 ,  C07D493/04 ,  C07F9/64

Abstract: Compounds and methods for inactivating pathogens in materials are described, including compositions and methods for inactivating pathogens in biological materials such as red blood cell preparations and plasma. The compounds and methods may be used to treat materials intended for in vitro or in vivo use, such as clinical testing or transfusion. The compounds are designed to specifically bind to and react with nucleic acid, and then to degrade to form breakdown products. The degradation reaction is preferably slower than the reaction with nucleic acid.

Abstract translation: 描述了用于灭活材料中的病原体的化合物和方法，包括用于灭活生物材料如红细胞制剂和血浆中的病原体的组合物和方法。 化合物和方法可以用于治疗用于体外或体内使用的物质，例如临床试验或输血。 该化合物被设计成与核酸特异性结合并与其反应，然后降解以形成分解产物。 降解反应优选比与核酸的反应更慢。

公开(公告)号：US07293985B2

公开(公告)日：2007-11-13

申请号：US10803109

申请日：2004-03-17

Applicant: David Cook ,  Adonis Stassinopoulos

Inventor： David Cook ,  Adonis Stassinopoulos

IPC: A01N1/02 ,  A01N63/00

CPC classification number: A61L2/0088

Abstract: Methods are provided for quenching undesired side reactions of pathogen inactivating compounds in biological materials comprising red blood cells. In a particular embodiment, methods are provided for quenching undesired side reactions of a pathogen inactivating compound that includes a functional group which is, or which is capable of forming, an electrophilic group. In this embodiment, the material is treated with the pathogen inactivating compound and a quencher, wherein the quencher comprises a nucleophilic functional group that is capable of covalently reacting with the electrophilic group. The electrophilic group on the pathogen inactivating compound is preferably a non-radical cationic group. In one embodiment, the pathogen inactivating compound includes a nucleic acid binding ligand and a mustard group, wherein the mustard group is capable of reacting in situ to form the electrophilic group. Preferred quenchers are thiols, such as glutathione. The methods permit inhibition of the modification of red blood cells in red blood cell containing materials during pathogen inactivation.

Abstract translation: 提供了用于猝灭病原体灭活化合物在包含红细胞的生物材料中的不希望的副反应的方法。 在一个具体实施方案中，提供了用于猝灭病原体灭活化合物的不希望的副反应的方法，其包括可亲电子基团或能够形成亲电基团的官能团。 在该实施方案中，用病原体灭活化合物和猝灭剂处理材料，其中猝灭剂包含能够与亲电子基团共价反应的亲核官能团。 病原体灭活化合物上的亲电基团优选为非自由基阳离子基团。 在一个实施方案中，病原体灭活化合物包括核酸结合配体和芥子基团，其中芥末基团能够在原位反应形成亲电子基团。 优选的猝灭剂是硫醇，如谷胱甘肽。 该方法允许在病原体灭活期间抑制含红细胞的材料中的红细胞的修饰。

公开(公告)号：US12045269B2

公开(公告)日：2024-07-23

申请号：US18378587

申请日：2023-10-10

Applicant: David Cook

Inventor： David Cook

IPC: G06F16/332 ,  G10L15/22 ,  H04L51/02

CPC classification number: G06F16/3329 ,  H04L51/02 ,  G10L15/22

Abstract: An apparatus for generating a digital assistant is disclosed. The apparatus include at least a processor and a memory communicatively connected to the at least a processor. The memory instructs the processor to receive at least one user query from a user. The memory instructs the processor to extract a plurality of background data and a plurality of contextual data from the user dataset. The memory instructs the processor to receive at least one user query from a user. The memory instructs the processor to generate a query response as a function of the at least one user query and the contextual data using the digital assistant. The memory instructs the processor to display the query response using the digital assistant on a display device.

公开(公告)号：US20240126794A1

公开(公告)日：2024-04-18

申请号：US18378587

申请日：2023-10-10

Applicant: David Cook

Inventor： David Cook

IPC: G06F16/332 ,  H04L51/02

CPC classification number: G06F16/3329 ,  H04L51/02

Abstract: An apparatus for generating a digital assistant is disclosed. The apparatus include at least a processor and a memory communicatively connected to the at least a processor. The memory instructs the processor to receive at least one user query from a user. The memory instructs the processor to extract a plurality of background data and a plurality of contextual data from the user dataset. The memory instructs the processor to receive at least one user query from a user. The memory instructs the processor to generate a query response as a function of the at least one user query and the contextual data using the digital assistant. The memory instructs the processor to display the query response using the digital assistant on a display device.

公开(公告)号：US11941563B1

公开(公告)日：2024-03-26

申请号：US17986375

申请日：2022-11-14

Applicant: David Cook

Inventor： David Cook

IPC: G06Q10/00 ,  G06Q10/0633 ,  G06Q50/02

CPC classification number: G06Q10/0633 ,  G06Q50/02

Abstract: An apparatus and method for fracking optimization, wherein the apparatus includes at least a processor, and a memory, wherein the memory containing instructions configuring the at least a processor to receive a reservoir datum from at least a sensing device, generate a production training data include a plurality of reservoir datums as input correlated to a plurality of optimal production parameters as output, train a fracking optimization machine-learning model using the production training data, determine an optimal production parameter as a function of the fracking optimization machine-learning model, and generating an optimal production plan as a function of the optimal production parameter.

公开(公告)号：US11880639B1

公开(公告)日：2024-01-23

申请号：US17986474

申请日：2022-11-14

Applicant: David Cook

Inventor： David Cook

IPC: G06F30/28

CPC classification number: G06F30/28

Abstract: An apparatus for multi-stage fracking, wherein the apparatus includes a pump configured to pump a fracking fluid into a rock region comprises a plurality of rock zones, and a computing device communicatively connected to the pump, wherein the computing device includes at least a processor, and a memory communicatively connected to the at least a processor containing instructions configuring the at least a processor to receive reservoir data, determine an optimal fracking stimulation parameter as a function of the reservoir data, identify a fracking stage as a function of the optimal fracking stimulation parameter, and adjust a pump configuration of the pump as a function of the fracking stage.

公开(公告)号：US09752481B2

公开(公告)日：2017-09-05

申请号：US14816331

申请日：2015-08-03

Applicant: David Cook

Inventor： David Cook

IPC: F01N3/20 ,  B61C3/02 ,  B61C17/02 ,  B61D15/06 ,  F01N3/10 ,  F01N13/08 ,  F01N13/10 ,  F01N13/18 ,  F01N13/00

CPC classification number: F01N3/20 ,  B61C3/02 ,  B61C17/02 ,  B61D15/06 ,  F01N3/103 ,  F01N3/2066 ,  F01N13/0093 ,  F01N13/085 ,  F01N13/10 ,  F01N13/1816 ,  F01N2340/02 ,  F01N2340/06 ,  F01N2610/02 ,  Y02T10/24 ,  Y02T30/12

Abstract: A system includes a prime engine connected to a prime engine exhaust stack that receives prime engine exhaust, a mixing duct section connected to the prime engine exhaust stack, a head-end power (HEP) generator connected to an HEP generator exhaust pipe that receives HEP generator exhaust, a single urea injector, and a selective catalytic reduction (SCR) system. The HEP generator exhaust pipe is connected to the mixing duct section, and the single urea injector injects urea into the HEP generator exhaust pipe upstream of the mixing duct section. The HEP generator exhaust and prime engine exhaust merge in the mixing duct section to form a merged exhaust that is received by the SCR system.