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1.发明授权Detection of binding reactions using labels detected by mediated catalytic electrochemistry 有权使用通过介导的催化电化学检测的标记检测结合反应公开(公告)号：US06346387B1
公开(公告)日：2002-02-12
申请号：US09722065
申请日：2000-11-24
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B82Y5/00 , C12Q1/6825 , G01N33/5438 , C12Q2565/607 , C12Q2563/137 , C12Q2563/113
摘要： A method of detecting binding interactions and target molecules, such as proteins, protein fragments, recombinant proteins, recombinant protein fragments, extracellular matrix proteins, ligands, carbohydrates, steroids, hormones, drugs, drug candidates, immunoglobulins and receptors of eukaryotic, prokaryotic or viral origin, by mediated electrochemistry using labels that react with transition metal mediator complexes in a detectable catalytic redox reaction. These labels are attached directly to binders, target molecules, surrogate target molecules, or to affinity ligands capable of binding to the target or to surrogate target molecules capable of competing with the target for binding to another binder. The labels can be naturally present (endogenous) in the binder, target or affinity ligand, or constructed by the covalent attachment of the label to the binder, target, affinity ligand or surrogate target (exogenous).
摘要翻译： 检测结合相互作用和靶分子的方法，例如蛋白质，蛋白质片段，重组蛋白，重组蛋白片段，细胞外基质蛋白，配体，碳水化合物，类固醇，激素，药物，候选药物，免疫球蛋白和真核，原核或病毒的受体 通过介导的电化学使用在可检测的催化氧化还原反应中与过渡金属介体络合物反应的标记。 这些标记物直接连接到粘合剂，靶分子，替代靶分子，或与能够结合靶的亲和配体或替代能与靶标竞争结合另一种粘合剂的靶分子。 标签可以在粘合剂，靶或亲和配体中天然存在（内源的），或者通过标记共价连接到粘合剂，靶，亲和配体或替代靶（外源的）上构建。
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公开(公告)号：US07202028B2
公开(公告)日：2007-04-10
申请号：US10237842
申请日：2002-09-09
CPC分类号： C12Q1/005 , C12Q1/004 , C12Q1/6825 , C12Q2565/101 , C12Q2563/113
摘要： A method of detecting two different target molecules through a single electrode is carried out by (a) providing a conductive oxidation-reduction reaction detection electrode; (b) contacting a sample suspected of containing a first and second target molecule to the electrode under conditions in which the first and second target molecules are deposited on the electrode, wherein the first target molecule comprises a first label and the second target molecule comprises a second label; (c) contacting to the electrode a first transition metal complex that oxidizes the first preselected label in a first oxidation-reduction reaction and a second transition metal complex that oxidizes the first and second labels in a second oxidation-reduction reaction, with the first and second oxidation-reduction reactions producing different detectable signals; (d) detecting the presence of the first target molecule by detecting the first oxidation-reduction reaction; and (e) detecting the presence of the second target molecule by detecting the second oxidation-reduction reaction. Devices for carrying out the method are also described.
摘要翻译： 通过（a）提供导电氧化还原反应检测电极来进行通过单个电极检测两种不同靶分子的方法; （b）将第一和第二靶分子沉积在电极上的条件下将疑似含有第一和第二靶分子的样品与电极接触，其中第一靶分子包含第一标记，第二靶分子包含 第二个标签 （c）在第一氧化还原反应中使第一预选标记物氧化的第一过渡金属络合物和在第二氧化还原反应中氧化第一和第二标记的第二过渡金属络合物与第一过渡金属络合物接触，第一和第 产生不同可检测信号的第二氧化还原反应; （d）通过检测第一氧化还原反应来检测第一靶分子的存在; 和（e）通过检测第二氧化还原反应来检测第二靶分子的存在。 还描述了用于执行该方法的装置。
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3.发明申请GLYCODELIN MONOCLONAL ANTIBODIES AND METHODS FOR THEIR USE IN THE DETECTION OF OVARIAN CANCER 有权谷维素单克隆抗体及其在检测卵巢癌中的应用公开(公告)号：US20130022998A1
公开(公告)日：2013-01-24
申请号：US13560080
申请日：2012-07-27
申请人： Robert L. Cheek , Eric P. Dixon , Timothy J. Fischer , John W. Groelke , Steven L. Knapp , Stephen G. Simkins , Clark M. Whitehead
发明人： Robert L. Cheek , Eric P. Dixon , Timothy J. Fischer , John W. Groelke , Steven L. Knapp , Stephen G. Simkins , Clark M. Whitehead
IPC分类号： G01N33/574
CPC分类号： C07K16/3069 , C07K16/18 , C07K2317/34 , G01N33/57449
摘要： Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to glycodelin. Monoclonal antibodies having the binding characteristics of a glycodelin antibody of the invention and monoclonal antibodies that bind to a glycodelin epitope of a disclosed antibody are further provided. Hybridoma cell lines that produce a glycodelin monoclonal antibody of the invention are also disclosed herein. Kits comprising one or more of the disclosed glycodelin monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for a glycodelin epitope of a disclosed monoclonal glycodelin antibody and methods of using these polypeptides in the production of glycodelin antibodies are also encompassed by the present invention.
摘要翻译： 提供了一种用于诊断患者卵巢癌和用于鉴定患卵巢癌可能性增加的患者的组合物和方法。 组合物包括特异性结合糖链蛋白的新型单克隆抗体及其变体和片段。 进一步提供具有本发明的糖链蛋白抗体的结合特征的单克隆抗体和与所公开的抗体的糖链蛋白表位结合的单克隆抗体。 本文还公开了产生本发明的糖链蛋白单克隆抗体的杂交瘤细胞系。 进一步提供包含一种或多种所公开的糖链素单克隆抗体并用于实施本发明方法的试剂盒。 包含所公开的单克隆糖蛋白抗体的糖链蛋白表位的氨基酸序列的多肽和在制备糖链蛋白抗体中使用这些多肽的方法也包括在本发明中。
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公开(公告)号：US20110038883A1
公开(公告)日：2011-02-17
申请号：US12915200
申请日：2010-10-29
IPC分类号： A61K39/00 , C07K16/18 , C12N5/16 , G01N33/53 , C07K7/06 , C07K7/08 , C07K14/00 , C07H21/04 , C12P21/06
CPC分类号： C07K16/3069 , C07K2317/34 , G01N33/57415 , G01N33/57449
摘要： Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to HE4. Monoclonal antibodies having the binding characteristics of an HE4 antibody of the invention are further provided. Hybridoma cell lines that produce an HE4 monoclonal antibody of the invention are also disclosed herein. The compositions find use in diagnostic methods as well as in screening methods for identifying patients having an increased likelihood of having ovarian cancer. Kits comprising one or more of the disclosed HE4 monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for an HE4 epitope and methods of using these polypeptides in the production of antibodies are also encompassed by the present invention.
摘要翻译： 提供了一种用于诊断患者卵巢癌和用于鉴定患卵巢癌可能性增加的患者的组合物和方法。 组合物包括特异性结合HE4的新型单克隆抗体及其变体和片段。 进一步提供具有本发明的HE4抗体的结合特性的单克隆抗体。 本文还公开了产生本发明的HE4单克隆抗体的杂交瘤细胞系。 该组合物用于诊断方法以及用于鉴定具有增加卵巢癌可能性的患者的筛选方法。 还提供了包含一种或多种所公开的HE4单克隆抗体和用于实施本发明的方法的试剂盒。 包含HE4表位的氨基酸序列的多肽和在产生抗体中使用这些多肽的方法也包括在本发明中。
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公开(公告)号：US20090081685A1
公开(公告)日：2009-03-26
申请号：US12268850
申请日：2008-11-11
IPC分类号： G01N33/574 , C12Q1/28 , C12Q1/02 , C12Q1/68
CPC分类号： G01N33/57449 , C12Q1/6886 , C12Q2600/112 , C12Q2600/158 , G01N33/57488 , G01N2333/4725
摘要： Methods and compositions for identifying ovarian cancer in a patient sample are provided. The methods of the invention comprise detecting overexpression of at least one biomarker in a body sample, wherein the biomarker is selectively overexpressed in ovarian cancer. In preferred embodiments, the body sample is a serum sample. The biomarkers of the invention include any genes or proteins that are selectively overexpressed in ovarian cancer, including, for example, acute phase reactants, lipoproteins, proteins involved in the regulation of the complement system, regulators of apoptosis, proteins that bind hemoglobin, heme, or iron, cytostructural proteins, enzymes that detoxify metabolic byproducts, growth factors, and hormone transporters. In some aspects of the invention, overexpression of a biomarker of interest is detected at the protein level using biomarker-specific antibodies or at the nucleic acid level using nucleic acid hybridization techniques. Kits for practicing the methods of the invention are further provided.
摘要翻译： 提供了用于鉴定患者样品中卵巢癌的方法和组合物。 本发明的方法包括检测身体样品中至少一种生物标志物的过表达，其中生物标志物在卵巢癌中选择性地过量表达。 在优选的实施方案中，身体样品是血清样品。 本发明的生物标志物包括在卵巢癌中选择性过表达的任何基因或蛋白质，包括例如急性期反应物，脂蛋白，参与调节补体系统的蛋白质，凋亡调节剂，结合血红蛋白的蛋白质，血红素， 或铁，细胞结构蛋白，解毒代谢副产物，生长因子和激素转运蛋白的酶。 在本发明的一些方面，使用生物标志物特异性抗体或在核酸水平使用核酸杂交技术在蛋白质水平上检测目标生物标志物的过表达。 还提供了用于实施本发明的方法的试剂盒。
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6.发明申请公开(公告)号：US20090075307A1
公开(公告)日：2009-03-19
申请号：US12261330
申请日：2008-10-30
申请人： Timothy J. Fischer , Douglas P. Malinowski , Qin He , Clark M. Whitehead , Robert L. Cheek , John W. Groelke
发明人： Timothy J. Fischer , Douglas P. Malinowski , Qin He , Clark M. Whitehead , Robert L. Cheek , John W. Groelke
IPC分类号： G01N33/53 , C12Q1/02 , G01N33/68 , G01N33/536
CPC分类号： G01N33/57449
摘要： Screening methods for identifying patients with an increased likelihood of having ovarian cancer are provided. The screening methods involve the detection of expression of a plurality of biomarkers in a body sample, wherein overexpression of the biomarkers is indicative of an increased likelihood of having ovarian cancer. The screening methods may further comprise a two-step analysis. Biomarkers of interest include genes and proteins that are, for example, involved in defects in DNA replication/cell cycle control, cell growth and proliferation, escape from apoptosis, angiogenesis or lymphogenesis, or the mechanisms of cancer cell motility and invasion. In some aspects of the invention, expression of a biomarker is detected at the protein level using a biomarker-specific antibody or at the nucleic acid level using nucleic acid hybridization techniques. Methods for detecting ovarian cancer in patients are further disclosed herein. Kits for practicing the methods of the invention are further provided.
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7.发明授权Glycodelin monoclonal antibodies and methods for their use in the detection of ovarian cancer 有权Glycodelin单克隆抗体及其用于检测卵巢癌的方法公开(公告)号：US08252904B2
公开(公告)日：2012-08-28
申请号：US12718693
申请日：2010-03-05
申请人： Robert L. Cheek , Eric P. Dixon , Timothy J. Fischer , John W. Groelke , Steven L. Knapp , Stephen G. Simkins , Clark M. Whitehead
发明人： Robert L. Cheek , Eric P. Dixon , Timothy J. Fischer , John W. Groelke , Steven L. Knapp , Stephen G. Simkins , Clark M. Whitehead
CPC分类号： C07K16/3069 , C07K16/18 , C07K2317/34 , G01N33/57449
摘要： Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to glycodelin. Monoclonal antibodies having the binding characteristics of a glycodelin antibody of the invention and monoclonal antibodies that bind to a glycodelin epitope of a disclosed antibody are further provided. Hybridoma cell lines that produce a glycodelin monoclonal antibody of the invention are also disclosed herein. The compositions find use in diagnostic methods as well as in screening methods for identifying patients having an increased likelihood of having ovarian cancer. Kits comprising one or more of the disclosed glycodelin monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for a glycodelin epitope of a disclosed monoclonal glycodelin antibody and methods of using these polypeptides in the production of glycodelin antibodies are also encompassed by the present invention.
摘要翻译： 提供了一种用于诊断患者卵巢癌和用于鉴定患卵巢癌可能性增加的患者的组合物和方法。 组合物包括特异性结合糖链蛋白的新型单克隆抗体及其变体和片段。 还提供具有本发明的糖链蛋白抗体的结合特征的单克隆抗体和结合所公开的抗体的糖链蛋白表位的单克隆抗体。 本文还公开了产生本发明的糖链蛋白单克隆抗体的杂交瘤细胞系。 该组合物用于诊断方法以及用于鉴定具有增加卵巢癌可能性的患者的筛选方法。 进一步提供包含一种或多种所公开的糖链素单克隆抗体并用于实施本发明方法的试剂盒。 包含所公开的单克隆糖蛋白抗体的糖链蛋白表位的氨基酸序列的多肽和在制备糖链蛋白抗体中使用这些多肽的方法也包括在本发明中。
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8.发明申请GLYCODELIN MONOCLONAL ANTIBODIES AND METHODS FOR THEIR USE IN THE DETECTION OF OVARIAN CANCER 有权谷维素单克隆抗体及其在检测卵巢癌中的应用公开(公告)号：US20100227343A1
公开(公告)日：2010-09-09
申请号：US12718693
申请日：2010-03-05
申请人： Robert L. Cheek , Eric P. Dixon , Timothy J. Fischer , John W. Groelke , Steven L. Knapp , Stephen G. Simkins , Clark M. Whitehead
发明人： Robert L. Cheek , Eric P. Dixon , Timothy J. Fischer , John W. Groelke , Steven L. Knapp , Stephen G. Simkins , Clark M. Whitehead
CPC分类号： C07K16/3069 , C07K16/18 , C07K2317/34 , G01N33/57449
摘要： Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to glycodelin. Monoclonal antibodies having the binding characteristics of a glycodelin antibody of the invention and monoclonal antibodies that bind to a glycodelin epitope of a disclosed antibody are further provided. Hybridoma cell lines that produce a glycodelin monoclonal antibody of the invention are also disclosed herein. The compositions find use in diagnostic methods as well as in screening methods for identifying patients having an increased likelihood of having ovarian cancer. Kits comprising one or more of the disclosed glycodelin monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for a glycodelin epitope of a disclosed monoclonal glycodelin antibody and methods of using these polypeptides in the production of glycodelin antibodies are also encompassed by the present invention.
摘要翻译： 提供了一种用于诊断患者卵巢癌和用于鉴定患卵巢癌可能性增加的患者的组合物和方法。 组合物包括特异性结合糖链蛋白的新型单克隆抗体及其变体和片段。 进一步提供具有本发明的糖链蛋白抗体的结合特征的单克隆抗体和与所公开的抗体的糖链蛋白表位结合的单克隆抗体。 本文还公开了产生本发明的糖链蛋白单克隆抗体的杂交瘤细胞系。 该组合物用于诊断方法以及用于鉴定具有增加卵巢癌可能性的患者的筛选方法。 进一步提供包含一种或多种所公开的糖链素单克隆抗体并用于实施本发明方法的试剂盒。 包含所公开的单克隆糖蛋白抗体的糖链蛋白表位的氨基酸序列的多肽和在制备糖链蛋白抗体中使用这些多肽的方法也包括在本发明中。
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9.发明申请METHODS FOR IDENTIFYING PATIENTS WITH AN INCREASED LIKELIHOOD OF HAVING OVARIAN CANCER AND COMPOSITIONS THEREFOR 审中-公开用于鉴别具有OVARIAN癌症和其组合物的增加的患者的患者的方法公开(公告)号：US20090068690A1
公开(公告)日：2009-03-12
申请号：US12261205
申请日：2008-10-30
申请人： Timothy J. Fischer , Douglas P. Malinowski , Qin He , Clark M. Whitehead , Robert L. Cheek , John W. Groelke
发明人： Timothy J. Fischer , Douglas P. Malinowski , Qin He , Clark M. Whitehead , Robert L. Cheek , John W. Groelke
CPC分类号： G01N33/57449
摘要： Screening methods for identifying patients with an increased likelihood of having ovarian cancer are provided. The screening methods involve the detection of expression of a plurality of biomarkers in a body sample, wherein overexpression of the biomarkers is indicative of an increased likelihood of having ovarian cancer. The screening methods may further comprise a two-step analysis. Biomarkers of interest include genes and proteins that are, for example, involved in defects in DNA replication/cell cycle control, cell growth and proliferation, escape from apoptosis, angiogenesis or lymphogenesis, or the mechanisms of cancer cell motility and invasion. In some aspects of the invention, expression of a biomarker is detected at the protein level using a biomarker-specific antibody or at the nucleic acid level using nucleic acid hybridization techniques. Methods for detecting ovarian cancer in patients are further disclosed herein. Kits for practicing the methods of the invention are further provided.
摘要翻译： 提供了鉴定患卵巢癌可能性增加的筛查方法。 筛选方法涉及检测体内样品中多种生物标志物的表达，其中生物标志物的过表达表明卵巢癌的可能性增加。 筛选方法还可以包括两步分析。 感兴趣的生物标志物包括例如参与DNA复制/细胞周期控制，细胞生长和增殖，逃避凋亡，血管生成或淋巴发生的缺陷或癌细胞运动和侵袭的机制的基因和蛋白质。 在本发明的一些方面，使用生物标志物特异性抗体或在核酸水平使用核酸杂交技术在蛋白质水平上检测生物标志物的表达。 本文进一步公开了患者卵巢癌检测方法。 还提供了用于实施本发明的方法的试剂盒。
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公开(公告)号：US20080254048A1
公开(公告)日：2008-10-16
申请号：US12044302
申请日：2008-03-07
IPC分类号： A61K39/00 , C07K16/18 , C12N5/06 , G01N33/574 , A61P37/00 , C12P21/04 , C07K14/00 , C07H21/00
CPC分类号： C07K16/3069 , C07K2317/34 , G01N33/57415 , G01N33/57449
摘要： Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to HE4. Monoclonal antibodies having the binding characteristics of an HE4 antibody of the invention are further provided. Hybridoma cell lines that produce an HE4 monoclonal antibody of the invention are also disclosed herein. The compositions find use in diagnostic methods as well as in screening methods for identifying patients having an increased likelihood of having ovarian cancer. Kits comprising one or more of the disclosed HE4 monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for an HE4 epitope and methods of using these polypeptides in the production of antibodies are also encompassed by the present invention.
摘要翻译： 提供了一种用于诊断患者卵巢癌和用于鉴定患卵巢癌可能性增加的患者的组合物和方法。 组合物包括特异性结合HE4的新型单克隆抗体及其变体和片段。 进一步提供具有本发明的HE4抗体的结合特性的单克隆抗体。 本文还公开了产生本发明的HE4单克隆抗体的杂交瘤细胞系。 该组合物用于诊断方法以及用于鉴定具有增加卵巢癌可能性的患者的筛选方法。 还提供了包含一种或多种所公开的HE4单克隆抗体和用于实施本发明的方法的试剂盒。 包含HE4表位的氨基酸序列的多肽和在产生抗体中使用这些多肽的方法也包括在本发明中。
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