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14 条记录 (耗时 0.052 秒)

    Methods for expression and purification of immunotoxins
    7.
    发明授权
    Methods for expression and purification of immunotoxins 有权
    免疫毒素的表达和纯化方法

    公开(公告)号：US08921097B2

    公开(公告)日：2014-12-30

    申请号：US12957165

    申请日：2010-11-30

    申请人： David M. Neville, Jr. ,  Jung-Hee Woo ,  Yuan-Yi Liu

    发明人： David M. Neville, Jr. ,  Jung-Hee Woo ,  Yuan-Yi Liu

    IPC分类号： C07K1/00 ,  C07K16/46 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C12N1/19

    CPC分类号： C07K1/18 ,  A61K47/6829 ,  A61K47/6849 ,  C07K16/2809 ,  C07K2317/622 ,  C07K2319/00 ,  C12N1/16

    摘要： The present invention relates to a method of expressing an immunotoxin in Pichia pastoris strain mutated to toxin resistance comprising a) growing the Pichia pastoris in a growth medium comprising an enzymatic digest of protein and yeast extract and maintaining a dissolved oxygen concentration at 40% and above; and b) performing methanol induction with a limited methanol feed of 0.5-0.75 ml/min/IO L of initial volume during induction along with a continuous infusion of yeast extract at a temperature below 17.5° C., antifoaming agent supplied up to 0.07%, agitation reduced to 400 RPM, and the induction phase extended out to 163 h.

    摘要翻译： 本发明涉及一种在对抗毒素抗性进行突变的巴斯德毕赤酵母菌株中表达免疫毒素的方法，包括：a）在含有蛋白质和酵母提取物的酶促消化物的生长培养基中培养巴斯德毕赤酵母，并将溶解氧浓度保持在40％以上 ; 和b）在诱导期间以0.5-0.75ml / min / IOL的初始体积的有限甲醇进料进行甲醇诱导，同时在低于17.5℃的温度下连续输注酵母提取物，消泡剂提供高达0.07％ ，搅拌降至400RPM，诱导期延长至163小时。

    METHODS FOR EXPRESSION AND PURIFICATION OF IMMUNOTOXINS
    9.
    发明申请
    METHODS FOR EXPRESSION AND PURIFICATION OF IMMUNOTOXINS 有权
    免疫细胞的表达和纯化方法

    公开(公告)号：US20130217861A1

    公开(公告)日：2013-08-22

    申请号：US12957165

    申请日：2010-11-30

    申请人： David M. Neville, JR. ,  Jung-Hee Woo ,  Yuan-Yi Liu

    发明人： David M. Neville, JR. ,  Jung-Hee Woo ,  Yuan-Yi Liu

    IPC分类号： C07K1/18

    CPC分类号： C07K1/18 ,  A61K47/6829 ,  A61K47/6849 ,  C07K16/2809 ,  C07K2317/622 ,  C07K2319/00 ,  C12N1/16

    摘要： The present invention relates to a method of expressing an immunotoxin in Pichia pastoris strain mutated to toxin resistance comprising a) growing the Pichia pastoris in a growth medium comprising an enzymatic digest of protein and yeast extract and maintaining a dissolved oxygen concentration at 40% and above; and b) performing methanol induction with a limited methanol feed of 0.5-0.75 ml/min/IO L of initial volume during induction along with a continuous infusion of yeast extract at a temperature below 17.5° C., antifoaming agent supplied up to 0.07%, agitation reduced to 400 RPM, and the induction phase extended out to 163 h.

    摘要翻译： 本发明涉及一种在对抗毒素抗性进行突变的巴斯德毕赤酵母菌株中表达免疫毒素的方法，包括：a）在含有蛋白质和酵母提取物的酶促消化物的生长培养基中培养巴斯德毕赤酵母，并将溶解氧浓度保持在40％以上 ; 和b）在诱导期间以0.5-0.75ml / min / IOL的初始体积的有限甲醇进料进行甲醇诱导，同时在低于17.5℃的温度下连续输注酵母提取物，消泡剂提供高达0.07％ ，搅拌降至400RPM，诱导期延长至163小时。

    Probiotic microorganisms producing chimeric human growth hormone fused with Fc fragment of human IgG for oral delivery system and methods for producing them
    10.
    发明申请
    Probiotic microorganisms producing chimeric human growth hormone fused with Fc fragment of human IgG for oral delivery system and methods for producing them 审中-公开
    与用于口服递送系统的人IgG的Fc片段融合的产生嵌合人生长激素的益生菌微生物及其制备方法

    公开(公告)号：US20060094083A1

    公开(公告)日：2006-05-04

    申请号：US11211464

    申请日：2005-08-26

    申请人： Yun-Jaie Choi ,  Chang-Hoon Lee ,  Jung-Hee Woo ,  Seung-Ha Kang ,  Seung-Hoon Choi ,  Sang-Kee Kang

    发明人： Yun-Jaie Choi ,  Chang-Hoon Lee ,  Jung-Hee Woo ,  Seung-Ha Kang ,  Seung-Hoon Choi ,  Sang-Kee Kang

    IPC分类号： C12P21/06 ,  C12N1/21 ,  C12N1/18 ,  C07K14/61

    CPC分类号： C07K14/61 ,  C07K2319/30 ,  C12N15/746 ,  C12N15/815

    摘要： The present invention relates to probiotic microorganisms producing chimeric human growth hormone for oral use and methods for preparing them. The invention provides probiotic Lactobacillus or yeast transformant expressing chimeric protein which is human growth hormone fused with Fc fragment of human IgG, in which the transformants are safely delivered into intestine though oral route. Also, the invention provides a chimeric protein-expressing vector which can induce transcytosis in intestine epithelial cells. Accordingly, the invention demonstrates that the chimeric protein for oral delivery system can be absorbed in intestine, and delivery of the chimeric protein by oral route using Lactobacillus has very excellent efficiency in vivo test in rats. Accordingly, the Lactobacillus of the present invention is an excellent deliverer of protein drugs.

    摘要翻译： 本发明涉及产生用于口服的嵌合人生长激素的益生菌微生物及其制备方法。 本发明提供了表达嵌合蛋白的益生菌乳杆菌或酵母转化体，其是与人IgG的Fc片段融合的人生长激素，其中转化体通过口服途径安全递送至肠。 此外，本发明提供了可诱导肠上皮细胞转胞吞作用的嵌合蛋白质表达载体。 因此，本发明表明用于口服递送系统的嵌合蛋白质可以在肠中被吸收，并且使用乳杆菌通过口服途径递送嵌合蛋白质具有非常优异的大鼠体内试验效率。 因此，本发明的乳杆菌是蛋白质药物的优良递送剂。