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公开(公告)号：US09364557B2
公开(公告)日：2016-06-14
申请号：US12671629
申请日：2008-08-01
CPC分类号： A61K47/48484 , A61K38/00 , A61K47/6829 , A61K47/6849 , A61K47/6855 , A61K47/6869 , A61K47/6879 , A61K2039/505
摘要： Provided are methods and compositions related to diphtheria toxin diabody immunotoxins.
摘要翻译： 提供与白喉毒素双抗体免疫毒素相关的方法和组合物。
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公开(公告)号：US08987426B2
公开(公告)日：2015-03-24
申请号：US13443779
申请日：2012-04-10
申请人： David M. Neville, Jr. , Jerry T. Thompson , Huaizhong Hu , Jung-Hee Woo , Shenglin Ma , Jonathan Mark Hexham , Mary Ellen Digan
发明人： David M. Neville, Jr. , Jerry T. Thompson , Huaizhong Hu , Jung-Hee Woo , Shenglin Ma , Jonathan Mark Hexham , Mary Ellen Digan
CPC分类号： C07K19/00 , A61K47/6829 , A61K47/6849 , C07K5/1008 , C07K7/06 , C07K7/08 , C07K14/34 , C07K16/2809 , C07K2317/24 , C07K2317/622 , C07K2319/00
摘要： The present invention described and shown in the specification and drawings provides novel recombinant DT-based immunotoxins, and, more specifically anti-T cell immunotoxin fusion proteins. Also provided are immunotoxins that can be expressed in bacterial, yeast, or mammalian cells. The invention also provides means for expression of the immunotoxin fusion protein. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims.
摘要翻译： 在说明书和附图中描述和示出的本发明提供了新的重组DT-基免疫毒素，更具体地，提供了抗T细胞免疫毒素融合蛋白。 还提供了可以在细菌，酵母或哺乳动物细胞中表达的免疫毒素。 本发明还提供了表达免疫毒素融合蛋白的方法。 要强调的是，该摘要被提供以符合要求抽象的规则，允许搜索者或其他读者快速确定技术公开内容的主题。 提交它的理解是，它不会用于解释或限制权利要求的范围或含义。
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公开(公告)号：US20130217861A1
公开(公告)日：2013-08-22
申请号：US12957165
申请日：2010-11-30
申请人： David M. Neville, JR. , Jung-Hee Woo , Yuan-Yi Liu
发明人： David M. Neville, JR. , Jung-Hee Woo , Yuan-Yi Liu
IPC分类号： C07K1/18
CPC分类号： C07K1/18 , A61K47/6829 , A61K47/6849 , C07K16/2809 , C07K2317/622 , C07K2319/00 , C12N1/16
摘要： The present invention relates to a method of expressing an immunotoxin in Pichia pastoris strain mutated to toxin resistance comprising a) growing the Pichia pastoris in a growth medium comprising an enzymatic digest of protein and yeast extract and maintaining a dissolved oxygen concentration at 40% and above; and b) performing methanol induction with a limited methanol feed of 0.5-0.75 ml/min/IO L of initial volume during induction along with a continuous infusion of yeast extract at a temperature below 17.5° C., antifoaming agent supplied up to 0.07%, agitation reduced to 400 RPM, and the induction phase extended out to 163 h.
摘要翻译： 本发明涉及一种在对抗毒素抗性进行突变的巴斯德毕赤酵母菌株中表达免疫毒素的方法，包括：a）在含有蛋白质和酵母提取物的酶促消化物的生长培养基中培养巴斯德毕赤酵母，并将溶解氧浓度保持在40％以上 ; 和b）在诱导期间以0.5-0.75ml / min / IOL的初始体积的有限甲醇进料进行甲醇诱导，同时在低于17.5℃的温度下连续输注酵母提取物，消泡剂提供高达0.07％ ，搅拌降至400RPM，诱导期延长至163小时。
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公开(公告)号：US08217158B2
公开(公告)日：2012-07-10
申请号：US12718673
申请日：2010-03-05
申请人： David M. Neville, Jr. , Jerry Todd Thompson , Huaizhong Hu , Jung-Hee Woo , Shenglin Ma , Jonathan Mark Hexham , Mary Ellen Digan
发明人： David M. Neville, Jr. , Jerry Todd Thompson , Huaizhong Hu , Jung-Hee Woo , Shenglin Ma , Jonathan Mark Hexham , Mary Ellen Digan
IPC分类号： C07H21/04
CPC分类号： C07K19/00 , A61K47/6829 , A61K47/6849 , C07K5/1008 , C07K7/06 , C07K7/08 , C07K14/34 , C07K16/2809 , C07K2317/24 , C07K2317/622 , C07K2319/00
摘要： The present invention described and shown in the specification and drawings provides novel recombinant DT-based immunotoxins, and, more specifically anti-T cell immunotoxin fusion proteins. Also provided are immunotoxins that can be expressed in bacterial, yeast, or mammalian cells. The invention also provides means for expression of the immunotoxin fusion protein. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims.
摘要翻译： 在说明书和附图中描述和示出的本发明提供了新的重组DT-基免疫毒素，更具体地，提供了抗T细胞免疫毒素融合蛋白。 还提供了可以在细菌，酵母或哺乳动物细胞中表达的免疫毒素。 本发明还提供了表达免疫毒素融合蛋白的方法。 要强调的是，该摘要被提供以符合要求抽象的规则，允许搜索者或其他读者快速确定技术公开内容的主题。 提交它的理解是，它不会用于解释或限制权利要求的范围或含义。
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公开(公告)号：US07696338B2
公开(公告)日：2010-04-13
申请号：US10296085
申请日：2001-05-18
申请人： David M. Neville, Jr. , Jerry T. Thompson , Huaizhong Hu , Jung-Hee Woo , Shenglin Ma , Jonathan Mark Hexham , Mary Ellen Digan
发明人： David M. Neville, Jr. , Jerry T. Thompson , Huaizhong Hu , Jung-Hee Woo , Shenglin Ma , Jonathan Mark Hexham , Mary Ellen Digan
IPC分类号： C07H21/04
CPC分类号： C07K19/00 , A61K47/6829 , A61K47/6849 , C07K5/1008 , C07K7/06 , C07K7/08 , C07K14/34 , C07K16/2809 , C07K2317/24 , C07K2317/622 , C07K2319/00
摘要： The present invention described and shown in the specification and drawings provides novel recombinant DT-based immunotoxins, and, more specifically anti-T cell immunotoxin fusion proteins. Also provided are immunotoxins that can be expressed in bacterial, yeast, or mammalian cells. The invention also provides means for expression of the immunotoxin fusion protein.
摘要翻译： 在说明书和附图中描述和示出的本发明提供了新的重组DT-基免疫毒素，更具体地，提供了抗T细胞免疫毒素融合蛋白。 还提供了可以在细菌，酵母或哺乳动物细胞中表达的免疫毒素。 本发明还提供了表达免疫毒素融合蛋白的方法。
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公开(公告)号：US5762927A
公开(公告)日：1998-06-09
申请号：US827772
申请日：1997-04-11
CPC分类号： C07K16/2809 , A61K38/164 , A61K39/39541 , A61K47/48238 , A61K47/48484 , A61K47/48561 , C07K14/34 , C07K2319/00
摘要： Disclosed is a method of inhibiting a rejection response by a primate to a transplanted organ. One exposes the primate to a mutant diphtheria toxin linked to anti-CD3 antibody so as to largely eliminate the host's peripheral blood T cell lymphocyte population. At the same time as, or after, the exposure step one administers to the primate's thymus gland donor lymphocytes. Transplantation of the organ follows. The primate is tolerized to the transplanted organ.
摘要翻译： 公开了一种抑制灵长类动物对移植器官的排斥反应的方法。 将灵长类动物暴露于与抗CD3抗体连接的突变白喉毒素，从而大大消除宿主的外周血T细胞淋巴细胞群体。 在同一时间或之后，暴露步骤一个施用于灵长类动物的胸腺供体淋巴细胞。 随机移植器官。 灵长类动物容忍移植器官。
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7.发明授权Protein crosslinking reagents cleavable within acidified intracellular vesicles 失效蛋白质交联试剂在酸化的细胞内可溶性蛋白
公开(公告)号：US5066490A
公开(公告)日：1991-11-19
申请号：US204163
申请日：1988-06-01
IPC分类号： A61K31/70 , A61K31/785 , A61K39/395 , A61K45/00 , A61K47/48 , A61P31/12 , C07D207/44 , C07D207/452 , C07D207/456 , C07D493/10 , C07K1/00 , C07K14/00 , C07K14/575 , C07K14/62 , C07K16/00
CPC分类号： C07D207/452 , A61K47/48338 , A61K47/48715 , C07D207/456 , C07D493/10
摘要： Crosslinking reagents for amino group-containing compounds are provided, which crosslinkers can be cleaved under mildly acidic conditions. The crosslinkers can be used to crosslink biologically active substances to be delivered to the cells, wherein the crosslinker will be cleaved in the mildly acidic conditions within the cell.
摘要翻译： 提供了含氨基化合物的交联试剂，该交联剂可在温和酸性条件下裂解。 交联剂可用于交联要传递至细胞的生物活性物质，其中交联剂将在细胞内的温和酸性条件下裂解。
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8.发明授权Treatment of graft versus host disease using a mixture of T-lymphocyte specific monoclonal antibody: ricin conjugates 失效使用T淋巴细胞特异性单克隆抗体混合物治疗移植物抗宿主病：蓖麻毒蛋白偶联物
公开(公告)号：US4520226A
公开(公告)日：1985-05-28
申请号：US456401
申请日：1983-01-07
摘要： A reagent and the protocol for the treatment of Graft Versus Host Disease is disclosed. Monoclonal antibodies specific for T-lymphocytes in human donor bone marrow are covalently linked to separate ricin toxin, combined in a mixture to form a treatment reagent, and combined with bone marrow removed from a human donor. The bone marrow-reagent mixture is then infused into an irradiated recipient. This protocol virtually eliminates T-lymphocyte activity, the cause of Graft Versus Host Disease.
摘要翻译： 公开了一种试剂和用于治疗移植物抗宿主病的方案。 在人供体骨髓中特异性T淋巴细胞的单克隆抗体与分离的蓖麻毒素共价连接，混合物混合形成治疗试剂，并与从人供体中移除的骨髓组合。 然后将骨髓 - 试剂混合物输入到照射的受体中。 该协议实际上消除了T淋巴细胞活性，是移植物与宿主病的原因。
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9.发明授权Monoclonal antibody-ricin hybrids as a treatment of murine graft-versus-host disease 失效单克隆抗体 - 蓖麻毒蛋白杂交体作为鼠移植物抗宿主病的治疗
公开(公告)号：US4440747A
公开(公告)日：1984-04-03
申请号：US350223
申请日：1982-02-19
CPC分类号： C07K16/2803 , A61K38/00 , Y10S530/868
摘要： The receptor specificity of toxins can be altered by coupling the intact toxin to monoclonal antibodies directed to the cell surface antigen Thy 1.2. Monoclonal antibody Thy 1.2-ricin (or ricin A chain) is a pretreatment reagent used to eliminate graft-versus-host disease (GVHD) in bone marrow transplants.
摘要翻译： 毒素的受体特异性可以通过将完整毒素与针对细胞表面抗原Thy 1.2的单克隆抗体偶联来改变。 单克隆抗体您的1.2蓖麻毒蛋白（或蓖麻毒素A链）是用于消除骨髓移植中的移植物抗宿主病（GVHD）的预处理试剂。
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公开(公告)号：US20110086416A1
公开(公告)日：2011-04-14
申请号：US12718673
申请日：2010-03-05
申请人： David M. Neville, Jr. , Jerry Todd Thompson , Huaizhong Hu , Jung-Hee Woo , Shenglin Ma , Jonathan Mark Hexham , Mary Ellen Digan
发明人： David M. Neville, Jr. , Jerry Todd Thompson , Huaizhong Hu , Jung-Hee Woo , Shenglin Ma , Jonathan Mark Hexham , Mary Ellen Digan
CPC分类号： C07K19/00 , A61K47/6829 , A61K47/6849 , C07K5/1008 , C07K7/06 , C07K7/08 , C07K14/34 , C07K16/2809 , C07K2317/24 , C07K2317/622 , C07K2319/00
摘要： The present invention described and shown in the specification and drawings provides novel recombinant DT-based immunotoxins, and, more specifically anti-T cell immunotoxin fusion proteins. Also provided are immunotoxins that can be expressed in bacterial, yeast, or mammalian cells. The invention also provides means for expression of the immunotoxin fusion protein. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims.
摘要翻译： 在说明书和附图中描述和示出的本发明提供了新的重组DT-基免疫毒素，更具体地，提供了抗T细胞免疫毒素融合蛋白。 还提供了可以在细菌，酵母或哺乳动物细胞中表达的免疫毒素。 本发明还提供了表达免疫毒素融合蛋白的方法。 要强调的是，该摘要被提供以符合要求抽象的规则，允许搜索者或其他读者快速确定技术公开内容的主题。 提交它的理解是，它不会用于解释或限制权利要求的范围或含义。
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