MICROFABRICATED QLIDA BIOSENSORS WITH AN EMBEDDED HEATING AND MIXING ELEMENT
    3.
    发明申请
    MICROFABRICATED QLIDA BIOSENSORS WITH AN EMBEDDED HEATING AND MIXING ELEMENT 审中-公开
    具有嵌入式加热和混合元件的微生物QLIDA生物传感器

    公开(公告)号:US20160282359A1

    公开(公告)日:2016-09-29

    申请号:US15172096

    申请日:2016-06-02

    摘要: An apparatus and method for detecting an analyte are described. The apparatus includes at least one microchannel adapted for an analyte to adhere to an interior surface thereof, a mixing element positioned within at least a portion of the at least one microchannel, a light source for energizing quantum dots conjugated with the analyte within the at least one microchannel, and a detection system for detecting and quantifying fluorescent energy emitted by the quantum dots in one or more predetermined wavelength ranges, wherein each wavelength range being correlated to one and only one type of analyte. The method includes the steps of providing a sample to at least one microchannel coated with an antibody, contacting the sample with a conjugate comprising a quantum dot and an antibody that specifically binds to the analyte, increasing electrothrermal flow of the sample, energizing the quantum dot with a light source, detecting fluorescent emission from the quantum dot, and correlating the fluorescent emission to the presence of or the concentration of the analyte in the sample.

    摘要翻译: 描述了用于检测分析物的装置和方法。 所述装置包括至少一个适于分析物粘附到其内表面上的微通道,位于所述至少一个微通道的至少一部分内的混合元件,用于激发与分析物共轭的量子点的光源 一个微通道和用于检测和量化在一个或多个预定波长范围内由量子点发射的荧光能量的检测系统,其中每个波长范围与一种且仅一种类型的分析物相关。 该方法包括以下步骤:将样品提供到涂覆有抗体的至少一个微通道,使样品与包含特异性结合分析物的量子点和抗体的缀合物接触,增加样品的电热流动,激发量子点 用光源检测来自量子点的荧光发射,并将荧光发射与样品中分析物的存在或浓度相关联。