公开(公告)号：US20220334106A1

公开(公告)日：2022-10-20

申请号：US17721558

申请日：2022-04-15

Applicant: DENSO CORPORATION

Inventor： Akira NUKAZUKA ,  Mana ASANO ,  Kei HAYAKAWA ,  Yuuya YOSHIMITSU ,  Kazuhiko KANO ,  Kazuhisa NAKAGAWA ,  Mai NIIMOTO

IPC: G01N33/53

Abstract: A conjugate includes a binding substance having an activity to bind to a target substance and a label causing a detectable phenomenon. A molecular weight of the binding substance is less than a molecular weight of an immunoglobulin.

公开(公告)号：US20240353407A1

公开(公告)日：2024-10-24

申请号：US18677132

申请日：2024-05-29

Applicant: DENSO CORPORATION

Inventor： Kei HAYAKAWA ,  Mana ASANO ,  Akira NUKAZUKA ,  Kazuhisa NAKAGAWA ,  Mai NIIMOTO ,  Kazuhiko KANO

IPC: G01N33/569 ,  G01N33/53 ,  G01N33/543

CPC classification number: G01N33/56983 ,  G01N33/5308 ,  G01N33/54386 ,  G01N2333/165 ,  G01N2469/10

Abstract: An analysis method for a target substance includes: mixing a conjugate and a sample including the target substance to produce a mixture, the conjugate including a labeled substance and a first binding substance having activity to bind to the target substance; bringing the mixture into contact with a second binding substance fixed to a base; and detecting a phenomenon caused by the labeled substance bound to the target substance that is bound to the second binding substance. The first binding substance is a substance including a nucleic acid, or a substance including an amino acid. The base is provided in each of regions. The target substance to which the second binding substance binds is different among the regions.

公开(公告)号：US20240309439A1

公开(公告)日：2024-09-19

申请号：US18677551

申请日：2024-05-29

Applicant: Denso Corporation

Inventor： Mai NIIMOTO ,  Akira Nukazuka ,  Mana Asano ,  Kazuhisa Nakagawa ,  Kei Hayakawa

IPC: C12Q1/6851 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6851 ,  C12Q1/6876 ,  C12Q1/6806

Abstract: An analysis method detects a target substance using a binding substance. The binding substance includes: a single-stranded nucleic acid preparation having activity to bind to the target substance; and a complementary nucleic acid that forms a base pair with a 3′ terminal region of the single-stranded nucleic acid preparation when the single-stranded nucleic acid preparation is not bound to the target substance, and that dissociates from the 3′ terminal region when the single-stranded nucleic acid preparation is bound to the target substance.

公开(公告)号：US20220334107A1

公开(公告)日：2022-10-20

申请号：US17721686

申请日：2022-04-15

Applicant: DENSO CORPORATION

Inventor： Akira NUKAZUKA ,  Mana ASANO ,  Kei HAYAKAWA ,  Kazuhiko KANO ,  Kazuhisa NAKAGAWA ,  Mai NIIMOTO

IPC: G01N33/53 ,  G01N33/543

Abstract: A method for detecting a plurality of target substances using a plurality of conjugates each including a binding substance and a label is provided. The binding substance has an activity to bind to one of the target substances and the label causes a detectable phenomenon. The method includes binding at least some of the plurality of conjugates with the plurality of target substances, removing a remainder of the plurality of conjugates that is not bound to the plurality of target substances, and detecting the label. A molecular weight of the binding substance is less than a molecular weight of an immunoglobulin.

公开(公告)号：US20240301476A1

公开(公告)日：2024-09-12

申请号：US18677250

申请日：2024-05-29

Applicant: DENSO CORPORATION

Inventor： Akira NUKAZUKA ,  Teppei SAKAI ,  Mana ASANO ,  Kei HAYAKAWA ,  Kazuhisa NAKAGAWA ,  Mai NIIMOTO

IPC: C12Q1/682 ,  C12N15/115 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N33/84

CPC classification number: C12Q1/682 ,  C12N15/115 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N33/84 ,  C12N2310/16

Abstract: An analysis method according to the present disclosure includes: mixing a binding substance and a sample including a target substance. The binding substance includes a nucleic acid region composed of a nucleic acid and having activity to bind to the target substance; removing the binding substance not bound to the target substance; amplifying the nucleic acid region; and detecting a phenomenon caused by the amplification of the nucleic acid region. For example, the analysis method includes: forming a complex of a template nucleic acid including a sequence complementary to a 3′ side sequence of the nucleic acid region; and amplifying the nucleic acid region by an action of a nucleic acid amplifying enzyme using the complex as a starting point.