公开(公告)号：US20240301476A1

公开(公告)日：2024-09-12

申请号：US18677250

申请日：2024-05-29

Applicant: DENSO CORPORATION

Inventor： Akira NUKAZUKA ,  Teppei SAKAI ,  Mana ASANO ,  Kei HAYAKAWA ,  Kazuhisa NAKAGAWA ,  Mai NIIMOTO

IPC: C12Q1/682 ,  C12N15/115 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N33/84

CPC classification number: C12Q1/682 ,  C12N15/115 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N33/84 ,  C12N2310/16

Abstract: An analysis method according to the present disclosure includes: mixing a binding substance and a sample including a target substance. The binding substance includes a nucleic acid region composed of a nucleic acid and having activity to bind to the target substance; removing the binding substance not bound to the target substance; amplifying the nucleic acid region; and detecting a phenomenon caused by the amplification of the nucleic acid region. For example, the analysis method includes: forming a complex of a template nucleic acid including a sequence complementary to a 3′ side sequence of the nucleic acid region; and amplifying the nucleic acid region by an action of a nucleic acid amplifying enzyme using the complex as a starting point.

公开(公告)号：US20240241078A1

公开(公告)日：2024-07-18

申请号：US18603999

申请日：2024-03-13

Applicant: DENSO CORPORATION

Inventor： Kazuhisa NAKAGAWA ,  Yuhei Shimizu ,  Daisuke Kobayashi ,  Masashi Suzuki ,  Akira Nukazuka ,  Teppei Sakai ,  Kei Hayakawa ,  Kazuhiko Kano ,  Mana Asano

IPC: G01N27/414 ,  G01N27/30

CPC classification number: G01N27/4141 ,  G01N27/301

Abstract: An ion sensor includes an ion detection element, a reference electrode that provides a reference point of a potential, and a solution in which an ion concentration can be changed in a sensing area of the ion detection element. The sensing area of the ion detection element is disposed in the solution. A method for detecting a target substance employs an ion sensor. The target substance bound to a label having a function of changing the amount of ions detectable by the ion sensor is introduced into the solution, and a change in the amount of ions is detected using the ion sensor.

公开(公告)号：US20220334106A1

公开(公告)日：2022-10-20

申请号：US17721558

申请日：2022-04-15

Applicant: DENSO CORPORATION

Inventor： Akira NUKAZUKA ,  Mana ASANO ,  Kei HAYAKAWA ,  Yuuya YOSHIMITSU ,  Kazuhiko KANO ,  Kazuhisa NAKAGAWA ,  Mai NIIMOTO

IPC: G01N33/53

Abstract: A conjugate includes a binding substance having an activity to bind to a target substance and a label causing a detectable phenomenon. A molecular weight of the binding substance is less than a molecular weight of an immunoglobulin.

公开(公告)号：US20240353407A1

公开(公告)日：2024-10-24

申请号：US18677132

申请日：2024-05-29

Applicant: DENSO CORPORATION

Inventor： Kei HAYAKAWA ,  Mana ASANO ,  Akira NUKAZUKA ,  Kazuhisa NAKAGAWA ,  Mai NIIMOTO ,  Kazuhiko KANO

IPC: G01N33/569 ,  G01N33/53 ,  G01N33/543

CPC classification number: G01N33/56983 ,  G01N33/5308 ,  G01N33/54386 ,  G01N2333/165 ,  G01N2469/10

Abstract: An analysis method for a target substance includes: mixing a conjugate and a sample including the target substance to produce a mixture, the conjugate including a labeled substance and a first binding substance having activity to bind to the target substance; bringing the mixture into contact with a second binding substance fixed to a base; and detecting a phenomenon caused by the labeled substance bound to the target substance that is bound to the second binding substance. The first binding substance is a substance including a nucleic acid, or a substance including an amino acid. The base is provided in each of regions. The target substance to which the second binding substance binds is different among the regions.

公开(公告)号：US20220334107A1

公开(公告)日：2022-10-20

申请号：US17721686

申请日：2022-04-15

Applicant: DENSO CORPORATION

Inventor： Akira NUKAZUKA ,  Mana ASANO ,  Kei HAYAKAWA ,  Kazuhiko KANO ,  Kazuhisa NAKAGAWA ,  Mai NIIMOTO

IPC: G01N33/53 ,  G01N33/543

Abstract: A method for detecting a plurality of target substances using a plurality of conjugates each including a binding substance and a label is provided. The binding substance has an activity to bind to one of the target substances and the label causes a detectable phenomenon. The method includes binding at least some of the plurality of conjugates with the plurality of target substances, removing a remainder of the plurality of conjugates that is not bound to the plurality of target substances, and detecting the label. A molecular weight of the binding substance is less than a molecular weight of an immunoglobulin.

公开(公告)号：US20170182950A1

公开(公告)日：2017-06-29

申请号：US15325106

申请日：2015-09-04

Applicant: DENSO CORPORATION

Inventor： Takahiro MIYAZAKI ,  Kazuhisa NAKAGAWA

IPC: B60R11/00 ,  H05K7/12 ,  B60R21/0136

CPC classification number: B60R11/00 ,  B60R21/0136 ,  B60R2011/004 ,  B60R2011/0059 ,  B60R2021/0006 ,  B60R2021/01006 ,  B60R2021/01027 ,  G01L1/04 ,  H05K7/12

Abstract: A temporary fixing unit includes a supporting portion including a distal end portion passed through an aperture, and a holding portion provided with an inclined surface that slides along an edge portion of the aperture when passed through the aperture. The temporary fixing unit also includes a spring portion connecting the distal end portion of the supporting portion and the holding portion and that deforms in a twisted manner as the holding portion is pushed down when the inclined surface of the holding portion slides along the edge portion of the aperture. When the holding portion is passed through the aperture, a portion of a door panel is held between the holding portion and an elastic portion. Furthermore, the elastic portion is elastically deformed so as to press the door panel.