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公开(公告)号：US08962277B2
公开(公告)日：2015-02-24
申请号：US13808380
申请日：2011-07-04
IPC分类号： C12P21/04 , C12P1/02 , C12N15/00 , C12N15/74 , C12N15/81 , C12N15/67 , C12N15/80 , C12P21/02
CPC分类号： C12N15/815 , C12N15/67 , C12N15/80 , C12P21/02
摘要： The invention refers to a method of producing a recombinant polypeptide of interest (POI) in a cell culture, comprising genetically engineering a eukaryotic cell line—to specifically cause prolongation of the G2+M cell cycle phase in a pre-culture phase, and—to produce the POI in a producing phase following the pre-culture phase, a high producer cell line and cell culture as well as a method of increasing the yield of a recombinant POI production in a cell culture.
摘要翻译： 本发明涉及一种在细胞培养物中生产重组感兴趣多肽（POI）的方法，其包括遗传工程化真核生物细胞系，以特异性地引起在预培养阶段G2 + M细胞周期的延长， 在预培养阶段之后的生产阶段产生POI，高生产细胞系和细胞培养物以及增加细胞培养物中重组POI产生的产量的方法。
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公开(公告)号：US20130137141A1
公开(公告)日：2013-05-30
申请号：US13808380
申请日：2011-07-04
IPC分类号： C12N15/81
CPC分类号： C12N15/815 , C12N15/67 , C12N15/80 , C12P21/02
摘要： The invention refers to a method of producing a recombinant polypeptide of interest (POI) in a cell culture, comprising genetically engineering a eukaryotic cell line—to specifically cause prolongation of the G2+M cell cycle phase in a pre-culture phase, and—to produce the POI in a producing phase following the pre-culture phase, a high producer cell line and cell culture as well as a method of increasing the yield of a recombinant POI production in a cell culture.
摘要翻译： 本发明涉及一种在细胞培养物中生产重组感兴趣多肽（POI）的方法，其包括遗传工程化真核生物细胞系，以特异性地引起在预培养阶段G2 + M细胞周期的延长， 在预培养阶段之后的生产阶段产生POI，高生产细胞系和细胞培养物以及增加细胞培养物中重组POI产生的产量的方法。
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公开(公告)号：US20100297738A1
公开(公告)日：2010-11-25
申请号：US12450705
申请日：2008-04-17
CPC分类号： C12N15/81 , C07K14/39 , C12N15/815 , C12P21/02
摘要： The present invention relates to methods for increasing the secretion of a protein of interest (POI) from a eukaryotic cell comprising co-expression of a POI and of at least one protein that enhances protein secretion, said enhancing protein being selected from the group consisting of BMH2, BFR2, C0G6, C0Y1, CUP5, IMH 1, KIN2, SEC31, SSA4 and SSE1. The invention further relates to a yeast promoter sequence, in particular to a promoter sequence of the PET9 gene of P. pastoris, having, under comparable conditions, an increased promoter activity relative to a promoter sequence of the GAP protein. The invention further relates to an expression vector comprising such a promoter sequence and to the use of such an expression vector for expression of a POI in a host cell. The invention further relates to new yeast promoter sequences of genes from P. pastoris, which are useful for expression of a POI in yeast.
摘要翻译： 本发明涉及用于增加来自真核细胞的目的蛋白质（POI）的分泌的方法，包括共表达POI和至少一种增强蛋白质分泌的蛋白质，所述增强蛋白选自 BMH2，BFR2，C0G6，C0Y1，CUP5，IMH1，KIN2，SEC31，SSA4和SSE1。 本发明进一步涉及酵母启动子序列，特别是巴斯德毕赤酵母的PET9基因的启动子序列，其在相当的条件下相对于GAP蛋白的启动子序列具有增加的启动子活性。 本发明还涉及包含这种启动子序列的表达载体以及这种表达载体在宿主细胞中表达POI的用途。 本发明还涉及来自巴斯德毕赤酵母的新酵母启动子序列，其可用于在酵母中表达POI。
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4.发明授权Methods for selecting a yeast population for the production of an organic acid and producing an organic acid 失效选择用于生产有机酸并生产有机酸的酵母种群的方法公开(公告)号：US07473540B2
公开(公告)日：2009-01-06
申请号：US11533507
申请日：2006-09-20
IPC分类号： C12P7/56
CPC分类号： C12Q1/04 , C12P7/40 , C12P7/56 , G01N33/569 , G01N2333/395
摘要： A method of producing an organic acid by staining a yeast population with a stain capable of internal pH (pHi)-dependent fluorescence, to yield a stained yeast population; determining a gate pH and a corresponding fluorescence parameter of the stained yeast population; and sorting the cells of the stained yeast population such that the cells having a pHi above the gate pH are retained and the cells having a pHi below the gate pH are discarded, to yield a yeast population for the production of the organic acid. Also, a method of producing an organic acid by performing the above steps, followed by isolating individual cells of the yeast population, to yield individual yeast cells for the production of an organic acid; culturing an individual yeast cell, to yield a cloned yeast population for the production of an organic acid; and incubating the cloned yeast population for the production of an organic acid in a medium containing an organic acid precursor, to produce the organic acid.
摘要翻译： 通过用能够内部pH（pHi） - 依赖性荧光的染色染色酵母菌群来产生有机酸的方法，以产生染色的酵母菌群; 确定染色酵母菌群的门pH和相应的荧光参数; 并且分选染色的酵母菌群的细胞，使得保留pH值高于门pH的细胞，并且将pHi低于门pH的细胞丢弃，以产生用于生产有机酸的酵母菌群。 另外，通过上述步骤生产有机酸的方法，然后分离出酵母菌群的各个细胞，得到用于生产有机酸的单独的酵母细胞; 培养单独的酵母细胞，产生用于生产有机酸的克隆酵母菌群; 并将克隆的酵母菌群培养在含有机酸前体的培养基中生产有机酸，生产有机酸。
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公开(公告)号：US20060234360A1
公开(公告)日：2006-10-19
申请号：US11105162
申请日：2005-04-13
摘要： Herein is disclosed a method of generating ascorbic acid from yeast transformed with a mannose epimerase. In a further embodiment, the yeast can be further transformed with a myoinositol phosphatase. In the method, the transformed yeast can produce L-ascorbic acid from D-glucose. The transformed yeast has been observed to have increased growth rate, cell density, or survival when cultured on appropriate media.
摘要翻译： 本文公开了从用甘露糖差向异构酶转化的酵母产生抗坏血酸的方法。 在另一个实施方案中，可以用肌醇磷酸酶进一步转化酵母。 在该方法中，转化的酵母可以从
D - 葡萄糖产生 L - 抗坏血酸。 已经观察到转化的酵母在适当的培养基上培养时具有增加的生长速率，细胞密度或存活。 -
公开(公告)号：US20120064630A1
公开(公告)日：2012-03-15
申请号：US13321318
申请日：2010-05-20
CPC分类号： C12N15/815
摘要： Invention relates to a eukaryotic host cell comprising a recombinant nucleotide sequence encoding an expression enhancer, which is selected from the group consisting of cLC52, RPL33 and cLC61, and its use in a method of producing a protein of interest (POI).
摘要翻译： 本发明涉及包含编码表达增强子的重组核苷酸序列的真核宿主细胞，所述重组核苷酸序列选自cLC52，RPL33和cLC61，以及其在生产目的蛋白质（POI）的方法中的用途。
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公开(公告)号：US20070141687A1
公开(公告)日：2007-06-21
申请号：US11546951
申请日：2006-10-12
摘要： A method of increasing stress tolerance in recombinant organisms that have been engineered for industrial production is described. Stress tolerance is increased by making L-ascorbic acid available to the recombinant organism, either by exogenous addition to the culture medium or by endogenous production from D-glucose by the recombinant organism. To enable endogenous production, the recombinant organism is transformed with a coding region encoding a mannose epimerase (ME), a coding region encoding an L-galactose dehydrogenase (LGDH), and a D-arabinono-1,4-lactone oxidase (ALO). The recombinant organism may be further transformed with a myoinositol phosphatase (MIP).
摘要翻译： 描述了为工业生产设计的重组生物体中增加胁迫耐受性的方法。 通过向培养基外源性加入或通过重组生物从D-葡萄糖的内源产生，通过使L-抗坏血酸可用于重组生物体来增加胁迫耐受性。 为了进行内源性生产，用编码甘露糖差向异构酶（ME），编码L-半乳糖脱氢酶（LGDH）的编码区和D-阿糖基-1,4-内酯氧化酶（ALO）的编码区转化重组生物， 。 可以用肌醇磷酸酶（MIP）进一步转化重组生物体。
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公开(公告)号：US20070065899A1
公开(公告)日：2007-03-22
申请号：US11533507
申请日：2006-09-20
CPC分类号： C12Q1/04 , C12P7/40 , C12P7/56 , G01N33/569 , G01N2333/395
摘要： A method of producing an organic acid by staining a yeast population with a stain capable of internal pH (pHi)-dependent fluorescence, to yield a stained yeast population; determining a gate pH and a corresponding fluorescence parameter of the stained yeast population; and sorting the cells of the stained yeast population such that the cells having a pHi above the gate pH are retained and the cells having a pHi below the gate pH are discarded, to yield a yeast population for the production of the organic acid. Also, a method of producing an organic acid by performing the above steps, followed by isolating individual cells of the yeast population, to yield individual yeast cells for the production of an organic acid; culturing an individual yeast cell, to yield a cloned yeast population for the production of an organic acid; and incubating the cloned yeast population for the production of an organic acid in a medium containing an organic acid precursor, to produce the organic acid.
摘要翻译： 通过用能够内部pH（pH <！ - SIPO
- >依赖性荧光的染色剂）染色酵母菌群来产生有机酸的方法，以产生染色的酵母菌群; 确定染色酵母菌群的门pH和相应的荧光参数; 并且分选染色的酵母菌群的细胞，使得具有高于门pH的pH <0.01的细胞被保留，并且将具有低于门pH的pH < ，以产生用于生产有机酸的酵母菌种。 另外，通过上述步骤生产有机酸的方法，然后分离出酵母菌群的各个细胞，得到用于生产有机酸的单独的酵母细胞; 培养单独的酵母细胞，产生用于生产有机酸的克隆酵母菌群; 并将克隆的酵母菌群培养在含有机酸前体的培养基中生产有机酸，生产有机酸。 -
公开(公告)号：US09561092B2
公开(公告)日：2017-02-07
申请号：US13356831
申请日：2012-01-24
申请人： Michael Sauer , Oliver Greve
发明人： Michael Sauer , Oliver Greve
IPC分类号： A61C17/22
CPC分类号： A61C17/222 , A61C17/22 , A61C17/225
摘要： An attachment for an oral hygiene device is disclosed. The attachment includes a housing; a shaft element movably arranged within the housing and arranged for being coupled to a drive shaft of a handle of the oral hygiene device; and a flexible sealing element attached to the shaft element. The flexible sealing element is arranged to seal a cavity within the attachment intended for accommodating at least a part of the drive shaft in an attached state.
摘要翻译： 公开了一种用于口腔卫生装置的附件。 附件包括壳体; 轴元件，其可移动地布置在所述壳体内并且布置成联接到所述口腔卫生装置的手柄的驱动轴; 以及附接到轴元件的柔性密封元件。 柔性密封元件布置成密封用于在附接状态下容纳至少一部分驱动轴的附件内的空腔。
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公开(公告)号：US09408682B2
公开(公告)日：2016-08-09
申请号：US12430487
申请日：2009-04-27
申请人： Titus Lanz , Michael Sauer
发明人： Titus Lanz , Michael Sauer
CPC分类号： A61D3/00 , A61B5/0555 , A61B6/03 , A61B2503/40
摘要： A device and a method for imaging an animal including an animal holding device for keeping the animal immobile and at a desired temperature during the scanning operation for imaging the animal in real-time in a non-destructive manner.
摘要翻译： 一种用于成像动物的装置和方法，所述装置和方法包括用于保持动物不动的动物保持装置，并且在扫描操作期间以非破坏性的方式实时成像动物。
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