利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

5 条记录 (耗时 0.018 秒)

    PLASMIDS AND PHAGES FOR HOMOLOGOUS RECOMBINATION AND METHODS OF USE
    3.
    发明申请
    PLASMIDS AND PHAGES FOR HOMOLOGOUS RECOMBINATION AND METHODS OF USE 有权
    用于同种异体重建的PLASMIDS和PHAGES及其使用方法

    公开(公告)号：US20100136692A1

    公开(公告)日：2010-06-03

    申请号：US12688764

    申请日：2010-01-15

    申请人： Donald L. Court ,  Simanti Datta ,  Nina Costantino

    发明人： Donald L. Court ,  Simanti Datta ,  Nina Costantino

    IPC分类号： C12N15/87 ,  C12N7/01

    CPC分类号： C12N15/74 ,  C12N7/00 ,  C12N15/70 ,  C12N15/73 ,  C12N2795/10321 ,  C12N2795/10343

    摘要： Lambda phages that can be used to introduce recombineering functions into host cells are disclosed. Also disclosed are plasmids that can be used to confer recombineering functions to a variety of strains of E. coli and to other bacteria, including Salmonella, Pseudomonas, Cyanobacteria, Spirochaetes. These plasmids and phages can be isolated in vitro and can be used to transform bacterial cells, such as gram negative bacteria.

    摘要翻译： 公开了可用于将重组功能引入宿主细胞的λ噬菌体。 还公开了可用于向多种大肠杆菌菌株和其它细菌（包括沙门氏菌，假单胞菌，蓝细菌，螺旋体）赋予重组功能的质粒。 这些质粒和噬菌体可以在体外分离，并可用于转化细菌细胞，如革兰氏阴性细菌。

    Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
    4.
    发明申请
    Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids 有权
    用于错配修复缺陷的宿主细胞及其在使用单链核酸诱导同源重组的方法中的用途

    公开(公告)号：US20050079618A1

    公开(公告)日：2005-04-14

    申请号：US10841125

    申请日：2004-05-07

    申请人： Donald Court ,  Xin-tian Li ,  Jian-Dong Huang ,  Nina Costantino ,  Depei Liu

    发明人： Donald Court ,  Xin-tian Li ,  Jian-Dong Huang ,  Nina Costantino ,  Depei Liu

    IPC分类号： C12N1/20 ,  C12N15/70 ,  C12N15/74

    CPC分类号： C12N1/20 ,  C12N15/70

    摘要： Methods are disclosed herein for inducing homologous recombination in a host cell comprising a target nucleic acid, using a single-stranded nucleic acid molecule. The single-stranded nucleic acid molecule has a sufficient number of nucleotides homologous to the target nucleic acid to enable homologous recombination with the target nucleic acid. The host cell includes a de-repressible promoter operably linked to a nucleic acid encoding a single-stranded binding protein and is deficient for mismatch repair. Isolated host cells of use in this method are also disclosed.

    摘要翻译： 本文公开了使用单链核酸分子在包含靶核酸的宿主细胞中诱导同源重组的方法。 单链核酸分子具有与目标核酸同源的足够数量的核苷酸，以实现与靶核酸的同源重组。 宿主细胞包括可操作地连接到编码单链结合蛋白的核酸并且缺陷以用于错配修复的去阻抑性启动子。 还公开了在该方法中使用的分离的宿主细胞。