公开(公告)号：US20180042969A1

公开(公告)日：2018-02-15

申请号：US15502451

申请日：2015-08-07

Applicant: Duke University

Inventor： Xiaowen Wang ,  Conrad P. Hodgkinson ,  Victor Dzau

IPC: A61K35/34 ,  A61K35/12

CPC classification number: A61K35/34 ,  A61K33/04 ,  A61K35/12 ,  A61K38/28 ,  A61K38/40 ,  A61P9/00 ,  C12N5/0657 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/65 ,  C12N2506/1307 ,  A61K2300/00

Abstract: The present disclosure provides compositions and methods for the reprogramming of cells such as fibroblasts into cardiomyocytes. The invention provided herein features a chemically defined media and methods of reprogramming cells to increase cardiac gene and protein expression in cardiac fibroblasts and other fibroblasts, e.g. dermal fibroblasts. The media and methods also enhance miR-combo mediated cardiac reprogramming of fibroblasts to cardiomyocytes. Thus, the invention encompasses a chemically defined reprogramming media comprising a base tissue culture media, insulin-transferrin-selenium (ITS) or ascorbic acid in a somatic cell-reprogramming, e.g., fibroblast-to-cardiomyocyte-reprogramming, amount.

公开(公告)号：US20170128526A1

公开(公告)日：2017-05-11

申请号：US15167058

申请日：2016-05-27

Applicant: The Brigham and Women's Hospital, Inc. ,  Duke University

Inventor： Victor Dzau ,  Maria Mirotsou

IPC: A61K38/17 ,  A61K45/06 ,  A61K9/00

CPC classification number: A61K38/1709 ,  A61K9/0019 ,  A61K9/0053 ,  A61K9/0085 ,  A61K38/17 ,  A61K38/18 ,  A61K45/06 ,  C07K14/435 ,  C07K14/475 ,  C12N5/10 ,  C12N15/09 ,  C12N15/63

Abstract: A purified paracrine factor of a mesenchymal stem cell, such as a Secreted frizzled related protein (Sfrp) is useful to reduce cell death an/or tissue injury associated with ischemic conditions.

公开(公告)号：US20200009197A1

公开(公告)日：2020-01-09

申请号：US16447672

申请日：2019-06-20

Applicant: Duke University

Inventor： Xiaowen Wang ,  Conrad P. Hodgkinson ,  Victor Dzau

IPC: A61K35/34 ,  A61K35/12

Abstract: The present disclosure provides compositions and methods for the reprogramming of cells such as fibroblasts into cardiomyocytes. The invention provided herein features a chemically defined media and methods of reprogramming cells to increase cardiac gene and protein expression in cardiac fibroblasts and other fibroblasts, e.g. dermal fibroblasts. The media and methods also enhance miR-combo mediated cardiac reprogramming of fibroblasts to cardiomyocytes. Thus, the invention encompasses a chemically defined reprogramming media comprising a base tissue culture media, insulin-transferrin-selenium (ITS) or ascorbic acid in a somatic cell-reprogramming, e.g., fibroblast-to-cardiomyocyte-reprogramming, amount.

公开(公告)号：US11512290B2

公开(公告)日：2022-11-29

申请号：US16982964

申请日：2019-03-21

Applicant: Duke University

Inventor： Conrad Hodgkinson ,  Victor Dzau ,  Jaewoo Lee ,  Bruce A. Sullenger

IPC: C07H21/02 ,  C12N5/077 ,  A61K31/713 ,  C12N15/113

Abstract: Disclosed herein are compositions and methods for cellular reprogramming. The compositions comprise one or more miRs and an activator of NFκB. Also provided are methods for enhancing or upregulating cardiomyocyte maturation in a cell or a subject and methods for inhibiting or downregulating cardiomyocyte maturation.