公开(公告)号：US20240197640A1

公开(公告)日：2024-06-20

申请号：US18474697

申请日：2023-09-26

Applicant: Duke University

Inventor： Conrad P. Hodgkinson ,  Hualing Sun ,  Victor J. Dzau

IPC: A61K9/50 ,  A61P9/00 ,  C12N5/071 ,  C12N15/113

CPC classification number: A61K9/5068 ,  A61P9/00 ,  C12N5/069 ,  C12N15/113 ,  C12N2310/141

Abstract: The present disclosure describes, in part, exosomes comprising miRNA for the reprogramming of fibroblasts and methods of using the same.

公开(公告)号：US20200009197A1

公开(公告)日：2020-01-09

申请号：US16447672

申请日：2019-06-20

Applicant: Duke University

Inventor： Xiaowen Wang ,  Conrad P. Hodgkinson ,  Victor Dzau

IPC: A61K35/34 ,  A61K35/12

Abstract: The present disclosure provides compositions and methods for the reprogramming of cells such as fibroblasts into cardiomyocytes. The invention provided herein features a chemically defined media and methods of reprogramming cells to increase cardiac gene and protein expression in cardiac fibroblasts and other fibroblasts, e.g. dermal fibroblasts. The media and methods also enhance miR-combo mediated cardiac reprogramming of fibroblasts to cardiomyocytes. Thus, the invention encompasses a chemically defined reprogramming media comprising a base tissue culture media, insulin-transferrin-selenium (ITS) or ascorbic acid in a somatic cell-reprogramming, e.g., fibroblast-to-cardiomyocyte-reprogramming, amount.

公开(公告)号：US20230313160A1

公开(公告)日：2023-10-05

申请号：US18054745

申请日：2022-11-11

Applicant: Duke University

Inventor： Conrad P. Hodgkinson ,  Hualing Sun ,  Richard Pratt ,  Victor J. Dzau

IPC: C12N9/22 ,  C12N15/86 ,  A61P9/12

CPC classification number: C12N9/22 ,  A61P9/12 ,  C12N15/86 ,  C12N2310/20 ,  C12N2750/14143

Abstract: The present disclosure describes compositions and methods for treating hypertension in a subject.

公开(公告)号：US20220175845A1

公开(公告)日：2022-06-09

申请号：US17541917

申请日：2021-12-03

Applicant: Duke University

Inventor： Conrad P. Hodgkinson ,  Victor J. Dzau

IPC: A61K35/33 ,  C12N15/113 ,  C12N5/077

Abstract: The invention provides a method of promoting reprogramming of a cardiac fibroblast into a cardiomyocyte by contacting the cardiac fibroblast with an isolated polynucleotide molecule comprising at least two functional miRNA sequences using a multicistronic expression system for reprogramming of fibroblasts into functional mature cardiomyocytes.

公开(公告)号：US12285444B2

公开(公告)日：2025-04-29

申请号：US17541917

申请日：2021-12-03

Applicant: Duke University

Inventor： Conrad P. Hodgkinson ,  Victor J. Dzau

IPC: A61K35/33 ,  C12N5/077 ,  C12N15/113

Abstract: The invention provides a method of promoting reprogramming of a cardiac fibroblast into a cardiomyocyte by contacting the cardiac fibroblast with an isolated polynucleotide molecule comprising at least two functional miRNA sequences using a multicistronic expression system for reprogramming of fibroblasts into functional mature cardiomyocytes.

公开(公告)号：US20180042969A1

公开(公告)日：2018-02-15

申请号：US15502451

申请日：2015-08-07

Applicant: Duke University

Inventor： Xiaowen Wang ,  Conrad P. Hodgkinson ,  Victor Dzau

IPC: A61K35/34 ,  A61K35/12

CPC classification number: A61K35/34 ,  A61K33/04 ,  A61K35/12 ,  A61K38/28 ,  A61K38/40 ,  A61P9/00 ,  C12N5/0657 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/65 ,  C12N2506/1307 ,  A61K2300/00

Abstract: The present disclosure provides compositions and methods for the reprogramming of cells such as fibroblasts into cardiomyocytes. The invention provided herein features a chemically defined media and methods of reprogramming cells to increase cardiac gene and protein expression in cardiac fibroblasts and other fibroblasts, e.g. dermal fibroblasts. The media and methods also enhance miR-combo mediated cardiac reprogramming of fibroblasts to cardiomyocytes. Thus, the invention encompasses a chemically defined reprogramming media comprising a base tissue culture media, insulin-transferrin-selenium (ITS) or ascorbic acid in a somatic cell-reprogramming, e.g., fibroblast-to-cardiomyocyte-reprogramming, amount.