公开(公告)号：US20150252428A1

公开(公告)日：2015-09-10

申请号：US14433460

申请日：2013-10-03

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Wayne Comper ,  Aparna Ramachandran ,  Haoheng Yan ,  Leileata M. Russo ,  Johan Karl Olav Skog

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present invention discloses methods for diagnosing or prognosing a disease or medical condition in a subject by detecting the presence or absence of BRAF mutant nucleic acids from nucleic acids extracted from microvesicles from a biological sample. The present invention also discloses methods for assessing the responsiveness or determining a treatment regimen for a subject in need thereof by detecting the presence or absence of BRAF mutant nucleic acids from nucleic acids extracted from microvesicles from a biological sample. Methods for isolating microvesicles and extracting DNA and/or RNA from the microvesicles are also described.

Abstract translation: 本发明公开了通过从生物样品的微泡提取的核酸中检测BRAF突变核酸的存在或不存在来诊断或预测受试者的疾病或医学病症的方法。 本发明还公开了通过从生物样品的微泡提取的核酸中检测BRAF突变核酸的存在或不存在来评价对有需要的受试者的反应性或确定治疗方案的方法。 还描述了分离微泡并从微泡中提取DNA和/或RNA的方法。

公开(公告)号：US11268085B2

公开(公告)日：2022-03-08

申请号：US16595278

申请日：2019-10-07

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US10465183B2

公开(公告)日：2019-11-05

申请号：US15325021

申请日：2015-07-09

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US20170198280A1

公开(公告)日：2017-07-13

申请号：US15325021

申请日：2015-07-09

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US20150353920A1

公开(公告)日：2015-12-10

申请号：US14759238

申请日：2014-01-03

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm ,  Johan Karl Olav Skog

IPC: C12N15/10

Abstract: The invention provides novel methods for isolating microvesicles from a biological sample and for extracting nucleic acids from the microvesicles.

Abstract translation: 本发明提供从生物样品中分离微泡并从微泡中提取核酸的新方法。