公开(公告)号：US12258587B2

公开(公告)日：2025-03-25

申请号：US17295287

申请日：2019-11-20

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg Stoll ,  Daniel Enderle ,  Mikkel Noerholm ,  Johan Karl Olov Skog

IPC: C12N15/10 ,  C12N7/00 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/70

Abstract: The present disclosure provides compositions, methods and kits for internal controls of microvesicle isolations. The compositions, methods and kits can comprise enveloped viruses, including, but not limited to, inactive mouse hepatitis virus (MHV).

公开(公告)号：US10808240B2

公开(公告)日：2020-10-20

申请号：US16301176

申请日：2017-05-15

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg Stoll ,  Daniel Enderle ,  Mikkel Noerholm ,  Johan Karl Olov Skog

IPC: C12N15/10 ,  C12N15/11 ,  C07H1/06

Abstract: The invention provides novel methods and kits for fully automated high-throughput method for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, novel methods and kits for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles that do not require the use of phenol or chloroform, and for extracting nucleic acids from the extracellular vesicles and/or from the biological samples.

公开(公告)号：US10301681B2

公开(公告)日：2019-05-28

申请号：US14909916

申请日：2014-08-06

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Mikkel Noerholm

IPC: C12Q1/6886 ,  G01N33/48

Abstract: The present invention relates generally to the field of biomarker analysis, particularly determining gene expression signatures from urine samples. The disclosure provides compositions, kits and methods for diagnosing a prostate disorder such as prostate cancer in a male subject.

公开(公告)号：US11136627B2

公开(公告)日：2021-10-05

申请号：US14424694

申请日：2013-08-30

Applicant: Exosome Diagnostics, Inc.

Inventor： Mikkel Noerholm ,  Susan Belzer ,  Charlotte Romain ,  Johan Karl Olov Skog ,  Leileata M. Russo ,  Wayne Comper

IPC: C12P19/34 ,  C12Q1/6886 ,  C12N7/00

Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.

公开(公告)号：US20170211139A1

公开(公告)日：2017-07-27

申请号：US15325499

申请日：2015-07-13

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Mikkel Noerholm ,  Daniel Enderle

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6848 ,  C12Q2527/146 ,  C12Q2535/122 ,  C12Q2545/101

Abstract: The present invention relates to compositions that include one or more control molecules known as artificial reference sequences and methods of using these control molecules for estimating rare nucleic acid sequence variants from low copy numbers in ultra-deep sequencing.

公开(公告)号：US12018334B2

公开(公告)日：2024-06-25

申请号：US17411606

申请日：2021-08-25

Applicant: Exosome Diagnostics, Inc.

Inventor： Mikkel Noerholm ,  Susan Belzer ,  Charlotte Romain ,  Johan Karl Olov Skog ,  Leileata M. Russo ,  Wayne Comper

IPC: C12P19/34 ,  C12N7/00 ,  C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12N7/00 ,  C12N2795/18122 ,  C12N2795/18131 ,  C12Q2600/106 ,  C12Q2600/118

Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.

公开(公告)号：US11268085B2

公开(公告)日：2022-03-08

申请号：US16595278

申请日：2019-10-07

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US10465183B2

公开(公告)日：2019-11-05

申请号：US15325021

申请日：2015-07-09

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US20170198280A1

公开(公告)日：2017-07-13

申请号：US15325021

申请日：2015-07-09

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US20150353920A1

公开(公告)日：2015-12-10

申请号：US14759238

申请日：2014-01-03

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm ,  Johan Karl Olav Skog

IPC: C12N15/10

Abstract: The invention provides novel methods for isolating microvesicles from a biological sample and for extracting nucleic acids from the microvesicles.

Abstract translation: 本发明提供从生物样品中分离微泡并从微泡中提取核酸的新方法。