公开(公告)号：US20160024491A1

公开(公告)日：2016-01-28

申请号：US14877531

申请日：2015-10-07

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olav Skog ,  Leileata M. Russo

IPC: C12N15/10

CPC classification number: C12N15/1003

Abstract: A method for extracting nucleic acids from a biological sample by isolating nucleic acid-containing particles from the biological sample by one or more centrifugation procedures, performing one or more steps to mitigate adverse factors that prevent or might prevent high quality nucleic acid extraction, and extracting nucleic acids from the isolated particles. The centrifugation procedures are performed at a speed not exceeding about 200,000 g. The extracted nucleic acids contain both 18S and 28S rRNA.

公开(公告)号：US20150252428A1

公开(公告)日：2015-09-10

申请号：US14433460

申请日：2013-10-03

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Wayne Comper ,  Aparna Ramachandran ,  Haoheng Yan ,  Leileata M. Russo ,  Johan Karl Olav Skog

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present invention discloses methods for diagnosing or prognosing a disease or medical condition in a subject by detecting the presence or absence of BRAF mutant nucleic acids from nucleic acids extracted from microvesicles from a biological sample. The present invention also discloses methods for assessing the responsiveness or determining a treatment regimen for a subject in need thereof by detecting the presence or absence of BRAF mutant nucleic acids from nucleic acids extracted from microvesicles from a biological sample. Methods for isolating microvesicles and extracting DNA and/or RNA from the microvesicles are also described.

Abstract translation: 本发明公开了通过从生物样品的微泡提取的核酸中检测BRAF突变核酸的存在或不存在来诊断或预测受试者的疾病或医学病症的方法。 本发明还公开了通过从生物样品的微泡提取的核酸中检测BRAF突变核酸的存在或不存在来评价对有需要的受试者的反应性或确定治疗方案的方法。 还描述了分离微泡并从微泡中提取DNA和/或RNA的方法。

公开(公告)号：US20150353920A1

公开(公告)日：2015-12-10

申请号：US14759238

申请日：2014-01-03

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm ,  Johan Karl Olav Skog

IPC: C12N15/10

Abstract: The invention provides novel methods for isolating microvesicles from a biological sample and for extracting nucleic acids from the microvesicles.

Abstract translation: 本发明提供从生物样品中分离微泡并从微泡中提取核酸的新方法。

公开(公告)号：US20150176073A1

公开(公告)日：2015-06-25

申请号：US14415990

申请日：2013-07-18

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olav Skog

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/702 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/166 ,  C12Q2527/125 ,  C12Q2563/161

Abstract: The invention provides a novel method for detecting the presence or absence of one or more transfer RNAs (tRNAs) contained in microvesicles from a subject. The invention also provides a novel method for detecting the presence or absence of one or more human endogenous retrovirus elements (HERV) in microvesicles from a subject. The methods disclosed is directed to aiding diagnosis, prognosis, minotring and evaluation of a disease or other medical condition in a subject.

Abstract translation: 本发明提供一种用于检测来自受试者的微泡中包含的一种或多种转移RNA（tRNA）的存在或不存在的新方法。 本发明还提供了一种用于检测来自受试者的微泡中一种或多种人内源性逆转录病毒元件（HERV）的存在或不存在的新方法。 所公开的方法旨在帮助患者的疾病或其他医学状况的诊断，预后，调节和评价。