公开(公告)号：US11407991B2

公开(公告)日：2022-08-09

申请号：US15138746

申请日：2016-04-26

Applicant: Exosome Diagnostics, Inc.

Inventor： Wayne Comper ,  Leileata M. Russo ,  Johan Karl Olov Skog

IPC: C07H21/00 ,  C12N15/10 ,  C07H21/02 ,  C07H1/08 ,  C12Q1/6806 ,  C12Q1/68 ,  G01N33/00

Abstract: Methods for extracting high quality nucleic acids from a heterogenous collection of nucleic acid-containing materials from a biological sample are disclosed. The heterogenous collection of nucleic-acid containing materials may contain cells or microvesicles, or both. The extractions obtained by the methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis, or therapy evaluation for a medical condition.

公开(公告)号：US11136627B2

公开(公告)日：2021-10-05

申请号：US14424694

申请日：2013-08-30

Applicant: Exosome Diagnostics, Inc.

Inventor： Mikkel Noerholm ,  Susan Belzer ,  Charlotte Romain ,  Johan Karl Olov Skog ,  Leileata M. Russo ,  Wayne Comper

IPC: C12P19/34 ,  C12Q1/6886 ,  C12N7/00

Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.

公开(公告)号：US20160237422A1

公开(公告)日：2016-08-18

申请号：US15138746

申请日：2016-04-26

Applicant: Exosome Diagnostics, Inc.

Inventor： Wayne Comper ,  Leileata M. Russo ,  Johan Karl Olov Skog

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  C07H1/08 ,  C07H21/02 ,  C12N15/1006 ,  C12N15/101 ,  C12Q1/6806 ,  C12Q2527/127 ,  C12Q2563/161

Abstract: Methods for extracting high quality nucleic acids from a heterogenous collection of nucleic acid-containing materials from a biological sample are disclosed. The heterogenous collection of nucleic-acid containing materials may contain cells or microvesicles, or both. The extractions obtained by the methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis, or therapy evaluation for a medical condition.

Abstract translation: 公开了从生物样品的含有核酸的材料的异质收集中提取高质量核酸的方法。 含有核酸的材料的异质收集可能含有细胞或微泡，或两者。 通过本文描述的方法获得的提取物的特征在于高产率和高完整性，使得提取的核酸可用于优先选择高质量核酸提取的各种应用，例如用于医疗状况的诊断，预后或治疗评估 。

公开(公告)号：US20150252428A1

公开(公告)日：2015-09-10

申请号：US14433460

申请日：2013-10-03

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Wayne Comper ,  Aparna Ramachandran ,  Haoheng Yan ,  Leileata M. Russo ,  Johan Karl Olav Skog

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present invention discloses methods for diagnosing or prognosing a disease or medical condition in a subject by detecting the presence or absence of BRAF mutant nucleic acids from nucleic acids extracted from microvesicles from a biological sample. The present invention also discloses methods for assessing the responsiveness or determining a treatment regimen for a subject in need thereof by detecting the presence or absence of BRAF mutant nucleic acids from nucleic acids extracted from microvesicles from a biological sample. Methods for isolating microvesicles and extracting DNA and/or RNA from the microvesicles are also described.

Abstract translation: 本发明公开了通过从生物样品的微泡提取的核酸中检测BRAF突变核酸的存在或不存在来诊断或预测受试者的疾病或医学病症的方法。 本发明还公开了通过从生物样品的微泡提取的核酸中检测BRAF突变核酸的存在或不存在来评价对有需要的受试者的反应性或确定治疗方案的方法。 还描述了分离微泡并从微泡中提取DNA和/或RNA的方法。

公开(公告)号：US12018334B2

公开(公告)日：2024-06-25

申请号：US17411606

申请日：2021-08-25

Applicant: Exosome Diagnostics, Inc.

Inventor： Mikkel Noerholm ,  Susan Belzer ,  Charlotte Romain ,  Johan Karl Olov Skog ,  Leileata M. Russo ,  Wayne Comper

IPC: C12P19/34 ,  C12N7/00 ,  C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12N7/00 ,  C12N2795/18122 ,  C12N2795/18131 ,  C12Q2600/106 ,  C12Q2600/118

Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.