公开(公告)号：US12258587B2

公开(公告)日：2025-03-25

申请号：US17295287

申请日：2019-11-20

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg Stoll ,  Daniel Enderle ,  Mikkel Noerholm ,  Johan Karl Olov Skog

IPC: C12N15/10 ,  C12N7/00 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/70

Abstract: The present disclosure provides compositions, methods and kits for internal controls of microvesicle isolations. The compositions, methods and kits can comprise enveloped viruses, including, but not limited to, inactive mouse hepatitis virus (MHV).

公开(公告)号：US20170211139A1

公开(公告)日：2017-07-27

申请号：US15325499

申请日：2015-07-13

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Mikkel Noerholm ,  Daniel Enderle

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6848 ,  C12Q2527/146 ,  C12Q2535/122 ,  C12Q2545/101

Abstract: The present invention relates to compositions that include one or more control molecules known as artificial reference sequences and methods of using these control molecules for estimating rare nucleic acid sequence variants from low copy numbers in ultra-deep sequencing.

公开(公告)号：US10808240B2

公开(公告)日：2020-10-20

申请号：US16301176

申请日：2017-05-15

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg Stoll ,  Daniel Enderle ,  Mikkel Noerholm ,  Johan Karl Olov Skog

IPC: C12N15/10 ,  C12N15/11 ,  C07H1/06

Abstract: The invention provides novel methods and kits for fully automated high-throughput method for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, novel methods and kits for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles that do not require the use of phenol or chloroform, and for extracting nucleic acids from the extracellular vesicles and/or from the biological samples.

公开(公告)号：US11268085B2

公开(公告)日：2022-03-08

申请号：US16595278

申请日：2019-10-07

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US10465183B2

公开(公告)日：2019-11-05

申请号：US15325021

申请日：2015-07-09

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US20170198280A1

公开(公告)日：2017-07-13

申请号：US15325021

申请日：2015-07-09

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US20150353920A1

公开(公告)日：2015-12-10

申请号：US14759238

申请日：2014-01-03

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm ,  Johan Karl Olav Skog

IPC: C12N15/10

Abstract: The invention provides novel methods for isolating microvesicles from a biological sample and for extracting nucleic acids from the microvesicles.

Abstract translation: 本发明提供从生物样品中分离微泡并从微泡中提取核酸的新方法。