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公开(公告)号：US20160237422A1
公开(公告)日：2016-08-18
申请号：US15138746
申请日：2016-04-26
Applicant: Exosome Diagnostics, Inc.
Inventor： Wayne Comper , Leileata M. Russo , Johan Karl Olov Skog
CPC classification number: C12N15/1003 , C07H1/08 , C07H21/02 , C12N15/1006 , C12N15/101 , C12Q1/6806 , C12Q2527/127 , C12Q2563/161
Abstract: Methods for extracting high quality nucleic acids from a heterogenous collection of nucleic acid-containing materials from a biological sample are disclosed. The heterogenous collection of nucleic-acid containing materials may contain cells or microvesicles, or both. The extractions obtained by the methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis, or therapy evaluation for a medical condition.
Abstract translation: 公开了从生物样品的含有核酸的材料的异质收集中提取高质量核酸的方法。 含有核酸的材料的异质收集可能含有细胞或微泡，或两者。 通过本文描述的方法获得的提取物的特征在于高产率和高完整性，使得提取的核酸可用于优先选择高质量核酸提取的各种应用，例如用于医疗状况的诊断，预后或治疗评估 。
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2.发明申请USE OF MICROVESICLES IN DIAGNOSIS, PROGNOSIS, AND TREATMENT OF MEDICAL DISEASES AND CONDITIONS 审中-公开在诊断，预防和治疗医疗疾病和病症中使用微血管公开(公告)号：US20150252428A1
公开(公告)日：2015-09-10
申请号：US14433460
申请日：2013-10-03
Applicant: EXOSOME DIAGNOSTICS, INC.
Inventor： Wayne Comper , Aparna Ramachandran , Haoheng Yan , Leileata M. Russo , Johan Karl Olav Skog
IPC: C12Q1/68
CPC classification number: C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/156 , C12Q2600/16
Abstract: The present invention discloses methods for diagnosing or prognosing a disease or medical condition in a subject by detecting the presence or absence of BRAF mutant nucleic acids from nucleic acids extracted from microvesicles from a biological sample. The present invention also discloses methods for assessing the responsiveness or determining a treatment regimen for a subject in need thereof by detecting the presence or absence of BRAF mutant nucleic acids from nucleic acids extracted from microvesicles from a biological sample. Methods for isolating microvesicles and extracting DNA and/or RNA from the microvesicles are also described.
Abstract translation: 本发明公开了通过从生物样品的微泡提取的核酸中检测BRAF突变核酸的存在或不存在来诊断或预测受试者的疾病或医学病症的方法。 本发明还公开了通过从生物样品的微泡提取的核酸中检测BRAF突变核酸的存在或不存在来评价对有需要的受试者的反应性或确定治疗方案的方法。 还描述了分离微泡并从微泡中提取DNA和/或RNA的方法。
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公开(公告)号：US11136627B2
公开(公告)日：2021-10-05
申请号：US14424694
申请日：2013-08-30
Applicant: Exosome Diagnostics, Inc.
Inventor： Mikkel Noerholm , Susan Belzer , Charlotte Romain , Johan Karl Olov Skog , Leileata M. Russo , Wayne Comper
IPC: C12P19/34 , C12Q1/6886 , C12N7/00
Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.
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Patent Agency Ranking
公开(公告)号：US11407991B2
公开(公告)日：2022-08-09
申请号：US15138746
申请日：2016-04-26
Applicant: Exosome Diagnostics, Inc.
Inventor： Wayne Comper , Leileata M. Russo , Johan Karl Olov Skog
Abstract: Methods for extracting high quality nucleic acids from a heterogenous collection of nucleic acid-containing materials from a biological sample are disclosed. The heterogenous collection of nucleic-acid containing materials may contain cells or microvesicles, or both. The extractions obtained by the methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis, or therapy evaluation for a medical condition.
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5.发明授权公开(公告)号：US10988755B2
公开(公告)日：2021-04-27
申请号：US14877531
申请日：2015-10-07
Applicant: Exosome Diagnostics, Inc.
Inventor： Johan Karl Olov Skog , Leileata M. Russo
IPC: C12N15/10
Abstract: A method for extracting nucleic acids from a biological sample by isolating nucleic acid-containing particles from the biological sample by one or more centrifugation procedures, performing one or more steps to mitigate adverse factors that prevent or might prevent high quality nucleic acid extraction, and extracting nucleic acids from the isolated particles. The centrifugation procedures are performed at a speed not exceeding about 200,000 g. The extracted nucleic acids contain both 18S and 28S rRNA.
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6.发明申请公开(公告)号：US20160024491A1
公开(公告)日：2016-01-28
申请号：US14877531
申请日：2015-10-07
Applicant: Exosome Diagnostics, Inc.
Inventor： Johan Karl Olav Skog , Leileata M. Russo
IPC: C12N15/10
CPC classification number: C12N15/1003
Abstract: A method for extracting nucleic acids from a biological sample by isolating nucleic acid-containing particles from the biological sample by one or more centrifugation procedures, performing one or more steps to mitigate adverse factors that prevent or might prevent high quality nucleic acid extraction, and extracting nucleic acids from the isolated particles. The centrifugation procedures are performed at a speed not exceeding about 200,000 g. The extracted nucleic acids contain both 18S and 28S rRNA.
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公开(公告)号：US20150024949A1
公开(公告)日：2015-01-22
申请号：US14508603
申请日：2014-10-07
Applicant: Exosome Diagnostics, Inc.
Inventor： Leileata M. Russo
IPC: C12Q1/68
CPC classification number: C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/158
Abstract: A method for detecting biomarkers of prostate cancer or other medical condition of the prostate based on the use of microvesicles obtained from urine samples, and the nucleic acids present in the microvesicles. The method disclosed herein are advantageous in that they may be used to support diagnosis, prognosis, monitoring, or therapy selection in lieu of or in conjunction with traditional biopsy-based diagnostics and do not require a digital rectal examination or prostate massage prior to urine sample collection.
Abstract translation: 基于从尿样获得的微泡的使用以及存在于微泡中的核酸，检测前列腺癌的生物标志物或前列腺的其它医学状况的方法。 本文公开的方法的优点在于它们可用于支持诊断，预后，监测或治疗选择，以代替传统的基于活检的诊断结合或结合传统的基于活检的诊断，并且不需要在尿液样本之前进行直肠指诊或前列腺按摩 采集。
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公开(公告)号：US12018334B2
公开(公告)日：2024-06-25
申请号：US17411606
申请日：2021-08-25
Applicant: Exosome Diagnostics, Inc.
Inventor： Mikkel Noerholm , Susan Belzer , Charlotte Romain , Johan Karl Olov Skog , Leileata M. Russo , Wayne Comper
IPC: C12P19/34 , C12N7/00 , C12Q1/6886
CPC classification number: C12Q1/6886 , C12N7/00 , C12N2795/18122 , C12N2795/18131 , C12Q2600/106 , C12Q2600/118
Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.
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