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公开(公告)号：US4493735A
公开(公告)日：1985-01-15
申请号：US397434
申请日：1982-07-12
申请人： Erwin Flaschel , Albert Renken
发明人： Erwin Flaschel , Albert Renken
CPC分类号： B01J19/32 , B01J8/20 , B01J8/34 , B01J2208/0084 , B01J2219/3221 , B01J2219/32217 , B01J2219/32272 , B01J2219/32466 , B01J2219/32475
摘要： A device for liquid-solid fluidized beds comprises a reactor in which a number of static mixing elements are disposed in superposed relation. A device of this kind, which is preferably used for chemical reactions, ensures high concentrations of solid and stable operation of the liquid-solid fluidized bed and has good radial exchange properties and reduced backmixing.
摘要翻译： 用于液固流化床的装置包括其中多个静态混合元件以重叠关系设置的反应器。 这种优选用于化学反应的装置确保液固固体流化床的高浓度的固体和稳定的操作，并且具有良好的径向交换性质和减少的回混。
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公开(公告)号：US4918016A
公开(公告)日：1990-04-17
申请号：US436423
申请日：1982-10-25
申请人： Jean-Louis Leuba , Albert Renken , Erwin Flaschel
发明人： Jean-Louis Leuba , Albert Renken , Erwin Flaschel
CPC分类号： C12N11/10 , A23C9/1206 , C12N11/14 , A23C2220/104
摘要： Enzymatic catalysts are produced from mineral particles coated with chitosan. The enzymes may be directly absorbed onto the surface after a cross-linking treatment to stablize the coating or immobilized by chemical bonds after a treatment with a bifunctional reagent, for example with glutaraldehyde. The stability of the biocatalyst is advantageously increased by retreatment with a bifunctional reagent after immobilization of the enzyme. Enzymes sensitive to glutaraldehyde are fixed by a carbodiimide. The catalysts thus prepared are insoluble in aqueous solutions. The particles are rigid and incompressible and are suitable for use in fixed-bed and fluidized-bed reactors. The immobilization yield may reach 90% depending on the circumstances.
摘要翻译： 酶催化剂由涂覆有壳聚糖的矿物颗粒制成。 在用双功能试剂（例如用戊二醛处理）之后，可以在交联处理之后将酶直接吸收到表面上以使涂层稳定或通过化学键固定。 生物催化剂的稳定性有利地通过在固定酶之后用双功能试剂再处理而增加。 对戊二醛敏感的酶由碳二亚胺固定。 如此制备的催化剂不溶于水溶液。 颗粒是刚性的和不可压缩的并且适用于固定床和流化床反应器。 根据情况，固定产量可达到90％。
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公开(公告)号：US07842481B2
公开(公告)日：2010-11-30
申请号：US11120812
申请日：2005-05-03
申请人： Carsten Voss , Erwin Flaschel
发明人： Carsten Voss , Erwin Flaschel
CPC分类号： C12N1/06
摘要： The present invention relates to a method, whereby microbial cells are broken up and cellular components are separated and an apparatus suitable for carrying out the method. Preferably, the cells contain extra-chromosomal nucleic acids that are released and are further suitable for additional purification as required for any pharmaceutical or technical use.
摘要翻译： 本发明涉及一种方法，其中微生物细胞被破碎并且细胞组分被分离，并且适用于实施该方法的装置。 优选地，细胞含有释放的染色体外核酸，并且进一步适用于任何药物或技术用途所需的额外纯化。
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公开(公告)号：US06664078B1
公开(公告)日：2003-12-16
申请号：US09700934
申请日：2000-11-21
申请人： Torsten Schmidt , Karl Friehs , Erwin Flaschel , Martin Schleef
发明人： Torsten Schmidt , Karl Friehs , Erwin Flaschel , Martin Schleef
IPC分类号： C12P1934
CPC分类号： C12P19/34 , C12N1/20 , C12N15/1003
摘要： The present invention relates to the production of biomass for the isolation of ccc plasmid DNA comprising culturing a bacterial transformant in a bioreactor containing an antibiotic-free batch medium under batch-conditions and, at the end of the batch phase, feeding under feed-back conditions the portion of a feed-back medium after the rise of the concentration of dissolved oxigen above a threshold-set point. Said feed-back medium comprises besides a carbon source a magnesium salt, preferably in concentrations above 20 mM. Preferably, the bacterial transformant is harvested after the end of the culture and frozen or freeze-dried. Also preferred is that ccc plasmid DNA is, optionally directly, isolated after harvesting the bacteria.
摘要翻译： 本发明涉及用于分离ccc质粒DNA的生物质的生产，包括在分批条件下在含有不含抗生素的批次培养基的生物反应器中培养细菌转化体，并且在分批阶段结束时，在反馈 在溶解氧化酶浓度上升超过阈值设定点之后，将反馈介质的部分调节。 所述反馈介质除碳源外还含有镁盐，优选浓度高于20mM。 优选地，在培养结束后收获细菌转化体并冷冻或冷冻干燥。 还优选的是，在收获细菌后，任选地直接分离ccc质粒DNA。
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公开(公告)号：US20050244947A1
公开(公告)日：2005-11-03
申请号：US11120812
申请日：2005-05-03
申请人： Carsten Voss , Erwin Flaschel
发明人： Carsten Voss , Erwin Flaschel
CPC分类号： C12N1/06
摘要： The present invention relates to a method, whereby microbial cells are broken up and cellular components are separated and an apparatus suitable for carrying out the method. Preferably, the cells contain extra-chromosomal nucleic acids that are released and are further suitable for additional purification as required for any pharmaceutical or technical use.
摘要翻译： 本发明涉及一种方法，其中微生物细胞被破碎并且细胞组分被分离，并且适用于实施该方法的装置。 优选地，细胞含有释放的染色体外核酸，并且进一步适用于任何药物或技术用途所需的额外纯化。
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