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公开(公告)号：US20110045572A1
公开(公告)日：2011-02-24
申请号：US12699979
申请日：2010-02-04
CPC分类号： C12N9/54 , A21D2/267 , A21D8/042 , A61K39/00 , C07K1/047 , C07K5/1008 , C07K5/101 , C07K5/1013 , C07K5/1019 , C07K5/1021 , C07K14/001 , C07K16/40 , C07K16/4283 , C11D3/38627 , C11D3/38645 , C12N9/0061 , C12N9/20 , C12Y110/03002 , C12Y304/21062 , Y10S435/911
摘要： The present invention relates to a method of selecting a protein variant having modified immunogenicity as compared to the parent protein comprising the steps obtaining antibody binding peptide sequences, using the sequences to localise epitope sequences on the 3-dimensional structure of parent protein, defining an epitope area including amino acids situated within 5 Å from the epitope amino acids constituting the epitope sequence, changing one or more of the amino acids defining the epitope area of the parent protein by genetical engineering mutations of a DNA sequence encoding the parent protein, introducing the mutated DNA sequence into a suitable host, culturing said host and expressing the protein variant, and evaluating the immunogenicity of the protein variant using the parent protein as reference. The invention further relates to the protein variant and use thereof, as well as to a method for producing said protein variant.
摘要翻译： 本发明涉及选择与亲本蛋白相比具有改良的免疫原性的蛋白质变体的方法，其包括获得抗体结合肽序列的步骤，使用该序列来定位母体蛋白的3维结构上的表位序列，限定表位 包括位于构成表位序列的表位氨基酸5埃范围内的氨基酸，通过编码母体蛋白质的DNA序列的遗传工程突变改变限定母体蛋白表位区域的一个或多个氨基酸，引入突变的 DNA序列转化为合适的宿主，培养所述宿主并表达蛋白质变体，并使用亲本蛋白作为参考来评估蛋白质变体的免疫原性。 本发明还涉及蛋白质变体及其用途，以及生产所述蛋白质变体的方法。
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公开(公告)号：US20120208251A1
公开(公告)日：2012-08-16
申请号：US13455905
申请日：2012-04-25
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
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公开(公告)号：US08617837B2
公开(公告)日：2013-12-31
申请号：US13455905
申请日：2012-04-25
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
摘要翻译： 本发明涉及亲本Termamyl样α-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质。
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公开(公告)号：US20110136206A1
公开(公告)日：2011-06-09
申请号：US13020545
申请日：2011-02-03
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
摘要翻译： 本发明涉及亲本Termamyl样α-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质。
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公开(公告)号：US07498158B2
公开(公告)日：2009-03-03
申请号：US10477725
申请日：2002-05-15
IPC分类号： C12N9/28 , C07K14/00 , A61K38/47 , C11D3/386 , C12N15/00 , C12N5/10 , C12N1/21 , C12P21/00 , C12Q1/40 , C07H21/00
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
摘要翻译： 本发明涉及亲本Termamyl样α-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质。
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公开(公告)号：US08486681B2
公开(公告)日：2013-07-16
申请号：US12360635
申请日：2009-01-27
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
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公开(公告)号：US5858757A
公开(公告)日：1999-01-12
申请号：US140083
申请日：1993-11-11
申请人： Claus Von Der Osten , Sven Branner , Allan Svendsen , Lisbeth Hedeg.ang.rd , Nina Eriksen , Maarten Robert Egmond , Eric Casteleijn
发明人： Claus Von Der Osten , Sven Branner , Allan Svendsen , Lisbeth Hedeg.ang.rd , Nina Eriksen , Maarten Robert Egmond , Eric Casteleijn
IPC分类号： C12N1/21 , C11D3/386 , C12N1/15 , C12N1/19 , C12N5/10 , C12N9/00 , C12N9/50 , C12N9/54 , C12N9/56 , C12N15/09 , C12N15/57 , C12R1/01 , C12R1/19 , C12N15/11 , C12N15/75
摘要： The invention relates to stabilized subtilisin proteases wherein one or more prolines is substituted for a native amino acid at stabilizing positions defined both by the range of the dihedral angles in the primary structure present at the substitution site and by selected characteristics of the protease secondary structure in the vicinity of the substitution site, to nucleotide sequences that encode the stabilized proteases, and to host organisms that contain the nucleotide sequences encoding the stabilized proteases.
摘要翻译： PCT No.PCT / DK92 / 00138 Sec。 371日期：1993年11月11日 102（e）日期1993年11月11日PCT提交1992年4月30日PCT公布。 出版物WO92 / 19729 日期1992年11月12日本发明涉及稳定的枯草杆菌蛋白酶，其中一个或多个脯氨酸取代了稳定位置处的天然氨基酸，两个稳定位置由在取代部位存在的一级结构中的二面角的范围和 在取代位点附近的蛋白酶二级结构，编码稳定化蛋白酶的核苷酸序列，以及含有编码稳定化蛋白酶的核苷酸序列的生物。
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公开(公告)号：US08252573B2
公开(公告)日：2012-08-28
申请号：US13020545
申请日：2011-02-03
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
摘要翻译： 本发明涉及亲本Termamyl样α-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质。
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公开(公告)号：US20090143270A1
公开(公告)日：2009-06-04
申请号：US12360635
申请日：2009-01-27
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-tike alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
摘要翻译： 本发明涉及亲本Termamyl-tikeα-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质。
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公开(公告)号：US5914306A
公开(公告)日：1999-06-22
申请号：US140008
申请日：1993-10-22
CPC分类号： C11D3/38645 , C11D3/386 , C11D3/38627 , C11D3/38636 , C11D3/38654 , C12N9/00 , C12N9/20
摘要： This invention relates to novel stabilized enzymes. More specifically the invention relates to novel stabilized enzymes, in which a naturally occurring amino acid residue (other than proline) has been substituted with a proline residue at one or more positions; at which position(s) the dihedral angles .phi. (phi) constitute values within the interval �-90.degree.
摘要翻译： PCT No.PCT / DK92 / 00142 Sec。 371日期：1993年10月22日 102（e）日期1993年10月22日PCT提交1992年5月1日PCT公布。 出版物WO92 / 19726 日期：1992年11月12日本发明涉及新型稳定化酶。 更具体地，本发明涉及新的稳定化酶，其中天然存在的氨基酸残基（除脯氨酸以外）已被一个或多个位置的脯氨酸残基取代; 在哪个位置上二面角phi（phi）构成间隔[-90°
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