公开(公告)号：US08198034B2

公开(公告)日：2012-06-12

申请号：US12403531

申请日：2009-03-13

申请人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

发明人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

IPC分类号： G01N33/53

CPC分类号： C07K16/1282 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C12Q1/37 ,  G01N33/5014 ,  G01N33/566 ,  G01N33/6854

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US20120122128A1

公开(公告)日：2012-05-17

申请号：US12403531

申请日：2009-03-13

申请人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

发明人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

IPC分类号： G01N33/566

CPC分类号： C07K16/1282 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C12Q1/37 ,  G01N33/5014 ,  G01N33/566 ,  G01N33/6854

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US20130040368A1

公开(公告)日：2013-02-14

申请号：US13649923

申请日：2012-10-11

申请人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton E. Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

发明人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton E. Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

IPC分类号： C12N9/52

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US08618261B2

公开(公告)日：2013-12-31

申请号：US12723474

申请日：2010-03-12

申请人： Fernandez-Salas Ester ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

发明人： Fernandez-Salas Ester ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

IPC分类号： C07K16/00

CPC分类号： C07K16/1282 ,  C07K2317/34 ,  C07K2317/77 ,  C12Q1/37 ,  G01N33/56911 ,  G01N2333/33 ,  G01N2333/705 ,  G01N2333/952

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合包含来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US20100203559A1

公开(公告)日：2010-08-12

申请号：US12723474

申请日：2010-03-12

申请人： Fernandez-Salas Ester ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

发明人： Fernandez-Salas Ester ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

IPC分类号： G01N33/53 ,  C07K7/08 ,  C07K16/18 ,  C07K7/06

CPC分类号： C07K16/1282 ,  C07K2317/34 ,  C07K2317/77 ,  C12Q1/37 ,  G01N33/56911 ,  G01N2333/33 ,  G01N2333/705 ,  G01N2333/952

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US08361789B2

公开(公告)日：2013-01-29

申请号：US12722801

申请日：2010-03-12

申请人： Hong Zhu ,  Joanne Wang ,  Birgitte P. S. Jacky ,  D. Dianne Hodges ,  Ester Fernandez-Salas

发明人： Hong Zhu ,  Joanne Wang ,  Birgitte P. S. Jacky ,  D. Dianne Hodges ,  Ester Fernandez-Salas

IPC分类号： C12N15/85

CPC分类号： C12N5/0618 ,  C12N5/0693 ,  G01N33/5014

摘要： The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines.

公开(公告)号：US20100233802A1

公开(公告)日：2010-09-16

申请号：US12722801

申请日：2010-03-12

申请人： Hong Zhu ,  Joanne Wang ,  Birgitte P.S. Jacky ,  D. Dianne Hodges ,  Ester Fernandez-Salas

发明人： Hong Zhu ,  Joanne Wang ,  Birgitte P.S. Jacky ,  D. Dianne Hodges ,  Ester Fernandez-Salas

IPC分类号： C12N5/10

CPC分类号： C12N5/0618 ,  C12N5/0693 ,  G01N33/5014

摘要： The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines.

摘要翻译： 本说明书公开了对BoNT / A中毒敏感的克隆细胞系，产生这种克隆细胞系的方法，以及使用这种克隆细胞系检测肉毒杆菌毒素血清型A活性的方法。

公开(公告)号：US08476068B2

公开(公告)日：2013-07-02

申请号：US13339166

申请日：2011-12-28

申请人： Hong Zhu ,  Joanne Wang ,  Birgitte P. S. Jacky ,  D. Dianne Hodges ,  Fernandez-Salas Ester

发明人： Hong Zhu ,  Joanne Wang ,  Birgitte P. S. Jacky ,  D. Dianne Hodges ,  Fernandez-Salas Ester

IPC分类号： C12N15/85

CPC分类号： C12N5/0618 ,  C12N5/0693 ,  G01N33/5014

摘要： The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines.

公开(公告)号：US20120156776A1

公开(公告)日：2012-06-21

申请号：US13339274

申请日：2011-12-28

申请人： Hong Zhu ,  Joanne Wang ,  Birgitte P.S. Jacky ,  D. Dianne Hodges ,  Fernandez-Salas Ester

发明人： Hong Zhu ,  Joanne Wang ,  Birgitte P.S. Jacky ,  D. Dianne Hodges ,  Fernandez-Salas Ester

IPC分类号： C12N5/07

CPC分类号： C12N5/0618 ,  C12N5/0693 ,  G01N33/5014

摘要： The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines.

公开(公告)号：US20120156774A1

公开(公告)日：2012-06-21

申请号：US13339211

申请日：2011-12-28

申请人： Hong Zhu ,  Joanne Wang ,  Birgitte P.S. Jacky ,  D. Dianne Hodges ,  Fernandez-Salas Ester

发明人： Hong Zhu ,  Joanne Wang ,  Birgitte P.S. Jacky ,  D. Dianne Hodges ,  Fernandez-Salas Ester

IPC分类号： C12N5/071 ,  C12N5/0793 ,  C12N5/09

CPC分类号： C12N5/0618 ,  C12N5/0693 ,  G01N33/5014

摘要： The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines.