公开(公告)号：US20090111100A1

公开(公告)日：2009-04-30

申请号：US11929884

申请日：2007-10-30

申请人： Eugene Lukhtanov ,  Noah Scarr

发明人： Eugene Lukhtanov ,  Noah Scarr

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C07H1/00 ,  C12P19/34

CPC分类号： C07H19/073 ,  C07H19/10 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q2565/1015 ,  C12Q2563/173 ,  C12Q2525/185

摘要： The incorporation of a minor groove binder spaced close to one member of a matched FRET set in a minor groove binder-oligonucleotide conjugate significantly reduces background fluorescence of a FRET probe or pair of probes and, consequently, increases the S/B ratios. Fluorescent-labeled probes are useful in carrying out hybridization, multiplex nucleic acid detection, and other procedures.

摘要翻译： 在较小沟槽粘合剂 - 寡核苷酸缀合物中，配合与匹配的FRET的一个成员隔开的小沟槽粘合剂的并入显着降低了FRET探针或一对探针的背景荧光，因此增加了S / B比。 荧光标记的探针可用于进行杂交，多重核酸检测和其他程序。

公开(公告)号：US20120015358A1

公开(公告)日：2012-01-19

申请号：US13185064

申请日：2011-07-18

申请人： Noah Scarr ,  Eugeny A. Lukhtanov

发明人： Noah Scarr ,  Eugeny A. Lukhtanov

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/04

CPC分类号： G06F19/20

摘要： The invention provides methods to identify pRNA- and pDNA oligomer affinity pairs. Affinity pairs comprised of nucleic acid oligomers which demonstrate no cross-reactivity (“orthogonal”) are designed using software and empirically verified by thermodynamic study and lateral flow testing. The design software uses a semi-random algorithm to build such sequences of nucleic acid oligomers based on user-input parameters for affinity strength and orthogonal stringency. These pairs can be applied for use in multi-analyte solid support and lateral flow diagnostic tests.

摘要翻译： 本发明提供鉴定pRNA和pDNA寡聚物亲和力对的方法。 使用软件设计的显示无交叉反应性（“正交”）的核酸寡聚体的亲和对是通过热力学研究和侧向流动测试经验验证的。 该设计软件使用半随机算法根据用户输入参数建立亲核强度和正交严格度的核酸寡聚体序列。 这些对可以应用于多分析物固体支持和侧流诊断测试。

公开(公告)号：US20120244535A1

公开(公告)日：2012-09-27

申请号：US13429273

申请日：2012-03-23

申请人： Alexei Vorobiev ,  Eugeny A. Lukhtanov ,  Noah Scarr

发明人： Alexei Vorobiev ,  Eugeny A. Lukhtanov ,  Noah Scarr

IPC分类号： C07H21/00 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6853 ,  C12Q1/6846 ,  C12Q2525/117

摘要： 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.

摘要翻译： 提供3-炔基肌苷类似物及其作为通用碱基的用途。 肌苷类似物可以并入核酸引物和探针中。 它们不会使核酸双链体显着不稳定。 因此，结合肌苷类似物的新型核酸引物和探针可以以多种方法使用。 类似物作为普遍基础出人意料地起作用。 它们不仅能够稳定双链体，而且与A，C，T和G相反的次黄嘌呤稳定，而且在引物中也被聚合酶识别，从而进行有效的扩增。

公开(公告)号：US10127349B2

公开(公告)日：2018-11-13

申请号：US13185064

申请日：2011-07-18

申请人： Noah Scarr ,  Eugeny A. Lukhtanov

发明人： Noah Scarr ,  Eugeny A. Lukhtanov

IPC分类号： G06F19/20 ,  G06F19/00

摘要： The invention provides methods to identify pRNA- and pDNA oligomer affinity pairs. Affinity pairs comprised of nucleic acid oligomers which demonstrate no cross-reactivity (“orthogonal”) are designed using software and empirically verified by thermodynamic study and lateral flow testing. The design software uses a semi-random algorithm to build such sequences of nucleic acid oligomers based on user-input parameters for affinity strength and orthogonal stringency. These pairs can be applied for use in multi-analyte solid support and lateral flow diagnostic tests.

公开(公告)号：US09085800B2

公开(公告)日：2015-07-21

申请号：US13429273

申请日：2012-03-23

申请人： Alexei Vorobiev ,  Eugeny A. Lukhtanov ,  Noah Scarr

发明人： Alexei Vorobiev ,  Eugeny A. Lukhtanov ,  Noah Scarr

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6853 ,  C12Q1/6846 ,  C12Q2525/117

摘要： 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.

摘要翻译： 提供3-炔基肌苷类似物及其作为通用碱基的用途。 肌苷类似物可以并入核酸引物和探针中。 它们不会使核酸双链体显着不稳定。 因此，结合肌苷类似物的新型核酸引物和探针可以以多种方法使用。 类似物作为普遍基础出人意料地起作用。 它们不仅能够稳定双链体，而且还要与A，C，T和G相反的次黄嘌呤稳定，但也可以通过聚合酶在引物中识别，从而进行有效的扩增。

公开(公告)号：US20130261014A1

公开(公告)日：2013-10-03

申请号：US13827456

申请日：2013-03-14

申请人： Alexei Vorobiev ,  Eugeny A. Lukhtanov ,  Noah Scarr

发明人： Alexei Vorobiev ,  Eugeny A. Lukhtanov ,  Noah Scarr

IPC分类号： C07H1/00

CPC分类号： C07H1/00 ,  C12Q1/6816 ,  C12Q1/6846 ,  C12Q2525/117

摘要： 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.

摘要翻译： 提供3-炔基肌苷类似物及其作为通用碱基的用途。 肌苷类似物可以并入核酸引物和探针中。 它们不会使核酸双链体显着不稳定。 因此，结合肌苷类似物的新型核酸引物和探针可以以多种方法使用。 类似物作为普遍基础出人意料地起作用。 它们不仅能够稳定双链体，而且与A，C，T和G相反的次黄嘌呤稳定，而且在引物中也被聚合酶识别，从而进行有效的扩增。