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公开(公告)号：US07169911B2
公开(公告)日：2007-01-30
申请号：US10651768
申请日：2003-08-29
申请人： Evelyn Durmaz , Todd R. Klaenhammer
发明人： Evelyn Durmaz , Todd R. Klaenhammer
CPC分类号： C07K14/195
摘要： A first aspect of the present invention is an isolated nucleic acid encoding a phage abortive defense protein, the isolated nucleic acid selected from the group consisting of: (a) isolated nucleic acid having the sequence or coding sequence given in SEQ ID NO: 1 (by “coding sequence” is meant nucleotides 373 to 1668 therein); (b) isolated nucleic acid encoding a phage abortive defense protein and which hybridizes to an nucleic acid having the sequence or coding sequence given in SEQ ID NO: 1, and/or having a sequence at least 60, 70, 80, 90, 92 or 95 percent identical to the sequence or coding sequence given in SEQ ID NO: 1; and (c) isolated nucleic acid encoding a phage abortive defense protein encoded by an isolated nucleic acid of (a) or (b) above, but differing in sequence therefrom due to the degeneracy of the genetic code (e.g., a nucleic acid encoding the protein given in SEQ ID NO: 2).
摘要翻译： 本发明的第一方面是编码噬菌体流产防御蛋白的分离核酸，所述分离的核酸选自：（a）具有SEQ ID NO：1中给出的序列或编码序列的分离的核酸（ “编码序列”是指其中的核苷酸373〜1668）; （b）编码噬菌体堕胎防御蛋白的分离的核酸，并且与具有SEQ ID NO：1中给出的序列或编码序列的核酸杂交，和/或具有至少60,70,80,90,92 或与SEQ ID NO：1中给出的序列或编码序列95％相同; 编码由上述（a）或（b）分离的核酸编码的噬菌体流产防御蛋白的分离的核酸，但是由于遗传密码的简并性（例如，编码 蛋白质，如SEQ ID NO：2所示）。
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公开(公告)号：US09040302B2
公开(公告)日：2015-05-26
申请号：US13697860
申请日：2011-05-19
申请人： Todd R. Klaenhammer , Richard L. Guerrant , Glynis L. Kolling , Evelyn Durmaz , Michael P. Timko , Cirle Alcantara Warren
发明人： Todd R. Klaenhammer , Richard L. Guerrant , Glynis L. Kolling , Evelyn Durmaz , Michael P. Timko , Cirle Alcantara Warren
CPC分类号： A61K38/164 , A23C9/1238 , A23L33/135 , A61K35/74 , A61K2035/11 , C12N1/06 , C12P21/02
摘要： Methods and compositions for targeted delivery of biotherapeutics are provided. The compositions comprise bile-sensitive St. thermophilus bacteria modified to release a biotherapeutic agent following bile exposure. Biotherapeutic agents released by the St. thermophilus bacteria disclosed herein include AQ and AQR rich peptides. Methods of the invention comprise administering to a subject a St. thermophilus bacterium modified to release a biotherapeutic agent following bile exposure. Administration of the St. thermophilus bacterium promotes a desired therapeutic response. The bacterium may be modified to express and release AQ or AQR rich peptides which subsequently inhibit cellular apoptosis or reduce mucosal damage. Thus, methods of the invention find use in treating or preventing a variety of gastrointestinal disorders including C. difficile infection and antibiotic-associated diarrhea.
摘要翻译： 提供用于靶向递送生物治疗剂的方法和组合物。 组合物包含胆汁敏感的嗜热链球菌，其被修饰以在胆汁暴露后释放生物治疗剂。 由本文公开的嗜热链球菌细菌释放的生物治疗剂包括AQ和AQR丰富的肽。 本发明的方法包括向受试者施用在胆汁暴露后经修饰以释放生物治疗剂的嗜热链球菌。 圣氏嗜热链霉菌的施用促进期望的治疗反应。 可以修饰细菌以表达和释放富含AQ或AQR的肽，其随后抑制细胞凋亡或减少粘膜损伤。 因此，本发明的方法用于治疗或预防各种胃肠疾病，包括艰难梭菌感染和抗生素相关性腹泻。
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公开(公告)号：US20130259834A1
公开(公告)日：2013-10-03
申请号：US13697860
申请日：2011-05-19
申请人： Todd R. Klaenhammer , Richard L. Guerrant , Glynis L. Kolling , Evelyn Durmaz , Michael P. Timko , Cirle Alcantara Warren
发明人： Todd R. Klaenhammer , Richard L. Guerrant , Glynis L. Kolling , Evelyn Durmaz , Michael P. Timko , Cirle Alcantara Warren
CPC分类号： A61K38/164 , A23C9/1238 , A23L33/135 , A61K35/74 , A61K2035/11 , C12N1/06 , C12P21/02
摘要： Methods and compositions for targeted delivery of biotherapeutics are provided. The compositions comprise bile-sensitive St. thermophilus bacteria modified to release a biotherapeutic agent following bile exposure. Biotherapeutic agents released by the St. thermophilus bacteria disclosed herein include AQ and AQR rich peptides. Methods of the invention comprise administering to a subject a St. thermophilus bacterium modified to release a biotherapeutic agent following bile exposure. Administration of the St. thermophilus bacterium promotes a desired therapeutic response. The bacterium may be modified to express and release AQ or AQR rich peptides which subsequently inhibit cellular apoptosis or reduce mucosal damage. Thus, methods of the invention find use in treating or preventing a variety of gastrointestinal disorders including C. difficile infection and antibiotic-associated diarrhea.
摘要翻译： 提供了用于靶向递送生物治疗剂的方法和组合物。 组合物包含胆汁敏感的嗜热链球菌，其被修饰以在胆汁暴露后释放生物治疗剂。 由本文公开的嗜热链球菌细菌释放的生物治疗剂包括AQ和AQR丰富的肽。 本发明的方法包括向受试者施用在胆汁暴露后经修饰以释放生物治疗剂的嗜热链球菌。 圣氏嗜热链霉菌的施用促进期望的治疗反应。 可以修饰细菌以表达和释放富含AQ或AQR的肽，其随后抑制细胞凋亡或减少粘膜损伤。 因此，本发明的方法用于治疗或预防各种胃肠疾病，包括艰难梭菌感染和抗生素相关性腹泻。
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公开(公告)号：US20050130126A1
公开(公告)日：2005-06-16
申请号：US10651768
申请日：2003-08-29
申请人： Evelyn Durmaz , Todd Klaenhammer
发明人： Evelyn Durmaz , Todd Klaenhammer
IPC分类号： C07K14/195 , C12N1/00 , C12Q1/70 , C07H21/04 , C07K14/005 , C12N7/00
CPC分类号： C07K14/195
摘要： A first aspect of the present invention is an isolated nucleic acid encoding a phage abortive defense protein, the isolated nucleic acid selected from the group consisting of: (a) isolated nucleic acid having the sequence or coding sequence given in SEQ ID NO: 1 (by “coding sequence” is meant nucleotides 373 to 1668 therein); (b) isolated nucleic acid encoding a phage abortive defense protein and which hybridizes to an nucleic acid having the sequence or coding sequence given in SEQ ID NO: 1, and/or having a sequence at least 60, 70, 80, 90, 92 or 95 percent identical to the sequence or coding sequence given in SEQ ID NO: 1; and (c) isolated nucleic acid encoding a phage abortive defense protein encoded by an isolated nucleic acid of (a) or (b) above, but differing in sequence therefrom due to the degeneracy of the genetic code (e.g., a nucleic acid encoding the protein given in SEQ ID NO: 2).
摘要翻译： 本发明的第一方面是编码噬菌体流产防御蛋白的分离核酸，所述分离的核酸选自：（a）具有SEQ ID NO：1中给出的序列或编码序列的分离的核酸（ “编码序列”是指其中的核苷酸373〜1668）; （b）编码噬菌体堕胎防御蛋白的分离的核酸，并且与具有SEQ ID NO：1中给出的序列或编码序列的核酸杂交，和/或具有至少60,70,80,90,92 或与SEQ ID NO：1中给出的序列或编码序列95％相同; 编码由上述（a）或（b）分离的核酸编码的噬菌体流产防御蛋白的分离的核酸，但是由于遗传密码的简并性（例如，编码 蛋白质，如SEQ ID NO：2所示）。
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