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公开(公告)号：US06251590B1
公开(公告)日：2001-06-26
申请号：US09046920
申请日：1998-03-24
IPC分类号： C12Q168
CPC分类号： C12Q1/6809 , C12Q1/6827 , C12Q1/6837 , C12Q2539/105 , C12Q2537/113 , C12Q2521/301 , C12Q2565/501
摘要： The present invention is directed to a method for identifying and/or cloning within a biological sample alternatively spliced nucleic acid regions ocurring between two physiological conditions, comprising hybridizing RNA derived from a test condition with cDNA derived from the standard condition and further identifying and/or cloning nucleic acids corresponding to alternative forms of splicing.
摘要翻译： 本发明涉及用于鉴定和/或克隆在两个生理条件之间的可变剪接的核酸区域的生物样品中的克隆方法，包括将来自测试条件的RNA与衍生自标准条件的cDNA杂交，并进一步鉴定和/或 对应于替代形式的剪接的克隆核酸。
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公开(公告)号：US20100055682A1
公开(公告)日：2010-03-04
申请号：US12227374
申请日：2007-05-14
IPC分类号： C12Q1/68 , C07H21/02 , C07D219/08 , C07D211/06 , C07C271/44
CPC分类号： C12Q1/6883 , C12Q1/6806 , C12Q2600/158
摘要： This application concerns methods and compositions that can be used for detecting the Alzheimer disease in mammals, particularly in humans. It describes in particular serum markers for Alzheimer's disease and the way they are used for diagnostic procedures. It also concerns tools and/or kits that can be used for applying these procedures (reagents, probes, primers, antibodies, chips, cells, etc.) with the preparation thereof and the way to use them. The invention can be used to detect the presence or the progression of Alzheimer illness in mammals, including at early stages of the disease.
摘要翻译： 本申请涉及可用于检测哺乳动物，特别是人类中的阿尔茨海默病的方法和组合物。 它特别描述了阿尔茨海默病的血清标志物及其用于诊断程序的方法。 它还涉及可用于应用这些程序（试剂，探针，引物，抗体，芯片，细胞等）及其使用方法的工具和/或试剂盒。 本发明可用于检测哺乳动物中阿尔茨海默病的存在或进展，包括在疾病的早期阶段。
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公开(公告)号：US20100144555A1
公开(公告)日：2010-06-10
申请号：US12641194
申请日：2009-12-17
CPC分类号： C12N15/1072 , C12Q1/6809 , C12Q1/6886 , C12Q2600/106 , C12Q2600/136 , C12Q2600/142 , C12Q2539/105 , C12Q2565/501 , C12Q2537/113 , C12Q2521/301
摘要： The invention concerns a method for identifying and/or cloning nucleic acid regions representing qualitative differences associated with alternative splicing events and/or with insertions, deletions located in RNA transcribed genome regions, between two physiological situations, comprising either hybridization of RNA derived from the test situation with cDNA's derived from the reference situation and/or reciprocally, or double-strand hybridization of cDNA derived from the test situation with cDNA's derived from the reference situation; and identifying and/or cloning nucleic acids representing qualitative differences. The invention also concerns compositions or banks of nucleic acids representing qualitative differences between two physiological situations, obtainable by the above method, and their use as probe, for identifying genes or molecules of interest, or still for example in methods of pharmacogenomics, and profiling of molecules relative to their therapeutic and/or toxic effects. The invention further concerns the use of dysregulation of splicing RNA as markers for predicting molecule toxicity and/or efficacy, and as markers in pharmacogenomics.
摘要翻译： 本发明涉及用于鉴定和/或克隆代表与选择性剪接事件相关的定性差异的核酸区域的方法和/或在两个生理情境之间的位于RNA转录基因组区域中的插入，缺失的方法，其包括衍生自测试的RNA的杂交 来自参考情况的cDNA的情况和/或来自测试情况的cDNA的双链杂交和/或来自参考情况的cDNA的双链杂交; 以及鉴定和/或克隆代表定性差异的核酸。 本发明还涉及表示通过上述方法获得的两种生理情况之间的定性差异的核酸组合物或组，以及它们作为探针的用途，用于鉴定感兴趣的基因或分子，或者仍然例如在药物基因组学的方法中，以及 分子相对于其治疗和/或毒性作用。 本发明还涉及使用拼接RNA的调节作为用于预测分子毒性和/或功效的标记物，以及作为药物基因组学中的标记物。
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公开(公告)号：US20100144550A1
公开(公告)日：2010-06-10
申请号：US12622364
申请日：2009-11-19
IPC分类号： C40B40/06
CPC分类号： C12Q1/6809 , C12Q2537/113 , C12Q2539/105 , C12Q2565/501 , C12Q2521/301
摘要： The invention concerns a method for identifying and/or cloning nucleic acid regions representing qualitative differences associated with alternative splicing events and/or with insertions, deletions located in RNA transcribed genome regions, between two physiological situations, comprising either hybridization of RNA derived from the test situation with cDNA's derived from the reference situation and/or reciprocally, or double-strand hybridization of cDNA derived from the test situation with cDNA's derived from the reference situation; and identifying and/or cloning nucleic acids representing qualitative differences. The invention also concerns compositions or banks of nucleic acids representing qualitative differences between two physiological situations, obtainable by the above method, and their use as probe, for identifying genes or molecules of interest, or still for example in methods of pharmacogenomics, and profiling of molecules relative to their therapeutic and/or toxic effects. The invention further concerns the use of dysregulation of splicing RNA as markers for predicting molecule toxicity and/or efficacy, and as markers in pharmacogenomics.
摘要翻译： 本发明涉及用于鉴定和/或克隆代表与选择性剪接事件相关的定性差异的核酸区域的方法和/或在两个生理情境之间的位于RNA转录基因组区域中的插入，缺失的方法，其包括衍生自测试的RNA的杂交 来自参考情况的cDNA的情况和/或来自测试情况的cDNA的双链杂交和/或来自参考情况的cDNA的双链杂交; 以及鉴定和/或克隆代表定性差异的核酸。 本发明还涉及表示通过上述方法获得的两种生理情况之间的定性差异的核酸组合物或组，以及它们作为探针的用途，用于鉴定感兴趣的基因或分子，或者仍然例如在药物基因组学的方法中，以及 分子相对于其治疗和/或毒性作用。 本发明还涉及使用拼接RNA的调节作为用于预测分子毒性和/或功效的标记物，以及作为药物基因组学中的标记物。
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公开(公告)号：US08003375B2
公开(公告)日：2011-08-23
申请号：US12622364
申请日：2009-11-19
CPC分类号： C12Q1/6809 , C12Q2537/113 , C12Q2539/105 , C12Q2565/501 , C12Q2521/301
摘要： The invention concerns a method for identifying and/or cloning nucleic acid regions representing qualitative differences associated with alternative splicing events and/or with insertions, deletions located in RNA transcribed genome regions, between two physiological situations, comprising either hybridization of RNA derived from the test situation with cDNA's derived from the reference situation and/or reciprocally, or double-strand hybridization of cDNA derived from the test situation with cDNA's derived from the reference situation; and identifying and/or cloning nucleic acids representing qualitative differences. The invention also concerns compositions or banks of nucleic acids representing qualitative differences between two physiological situations, obtainable by the above method, and their use as probe, for identifying genes or molecules of interest, or still for example in methods of pharmacogenomics, and profiling of molecules relative to their therapeutic and/or toxic effects. The invention further concerns the use of dysregulation of splicing RNA as markers for predicting molecule toxicity and/or efficacy, and as markers in pharmacogenomics.
摘要翻译： 本发明涉及用于鉴定和/或克隆代表与选择性剪接事件相关的定性差异的核酸区域的方法和/或在两个生理情境之间的位于RNA转录基因组区域中的插入，缺失的方法，其包括衍生自测试的RNA的杂交 来自参考情况的cDNA的情况和/或来自测试情况的cDNA的双链杂交和/或来自参考情况的cDNA的双链杂交; 以及鉴定和/或克隆代表定性差异的核酸。 本发明还涉及表示通过上述方法获得的两种生理情况之间的定性差异的核酸组合物或组，以及它们作为探针的用途，用于鉴定感兴趣的基因或分子，或者仍然例如在药物基因组学的方法中，以及 分子相对于其治疗和/或毒性作用。 本发明还涉及使用拼接RNA的调节作为用于预测分子毒性和/或功效的标记物，以及作为药物基因组学中的标记物。
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公开(公告)号：US20090269744A1
公开(公告)日：2009-10-29
申请号：US12091835
申请日：2006-10-26
申请人： Alexander Krause , Philippe Leissner , Malick Paye , Bruno Mougin , Fabien Schweighoffer , Laurent Bracco
发明人： Alexander Krause , Philippe Leissner , Malick Paye , Bruno Mougin , Fabien Schweighoffer , Laurent Bracco
CPC分类号： C12Q1/6886 , C12Q2600/156 , C12Q2600/158
摘要： The present application concerns methods and compositions which can be used to detect cancer in mammals, in particular in humans. It notably describes serum markers of cancers and their uses in diagnosis methods. It also concerns tools and/or kits which can be used to implement these methods (reagents, probes, primers, antibodies, chips, cells, etc.), their preparation and their uses. The invention can be used to detect the presence or the progression of a cancer, particularly breast cancer, including at an early stage.
摘要翻译： 本申请涉及可用于检测哺乳动物，特别是人类中的癌症的方法和组合物。 它特别描述了癌症的血清标志物及其在诊断方法中的用途。 它还涉及可用于实施这些方法（试剂，探针，引物，抗体，芯片，细胞等）的工具和/或试剂盒，其制备及其用途。 本发明可用于检测癌症，特别是乳腺癌的存在或进展，包括早期阶段。
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公开(公告)号：US06881571B1
公开(公告)日：2005-04-19
申请号：US09623828
申请日：1999-03-11
CPC分类号： C12Q1/6809 , C12Q2537/113 , C12Q2539/105 , C12Q2565/501 , C12Q2521/301
摘要： The invention concerns a method for identifying and/or cloning nucleic acid regions representing qualitative differences associated with alternative splicing events and/or with insertions, deletions located in RNA transcribed genome regions, between two physiological situations, comprising either hybridization of RNA derived from the test situation with cDNA's derived from the reference situation and/or reciprocally, or double-strand hybridization of cDNA derived from the test situation with cDNA's derived from the reference situation; and identifying and/or cloning nucleic acids representing qualitative differences. The invention also concerns compositions or banks of nucleic acids representing qualitative differences between two physiological situations, obtainable by the above method, and their use as probe, for identifying genes or molecules of interest, or still for example in methods of pharmacogenomics, and profiling of molecules relative to their therapeutic and/or toxic effects. The invention further concerns the use of dysregulation of splicing RNA as markers for predicting molecule toxicity and/or efficacy, and as markers in pharmacogenomics.
摘要翻译： 本发明涉及用于鉴定和/或克隆代表与选择性剪接事件相关的定性差异的核酸区域的方法和/或在两个生理情境之间的位于RNA转录基因组区域中的插入，缺失的方法，其包括衍生自测试的RNA的杂交 来自参考情况的cDNA的情况和/或来自测试情况的cDNA的双链杂交和/或来自参考情况的cDNA的双链杂交; 以及鉴定和/或克隆代表定性差异的核酸。 本发明还涉及表示通过上述方法获得的两种生理情况之间的定性差异的核酸组合物或组，以及它们作为探针的用途，用于鉴定感兴趣的基因或分子，或者仍然例如在药物基因组学的方法中，以及 分子相对于其治疗和/或毒性作用。 本发明还涉及使用拼接RNA的调节作为用于预测分子毒性和/或功效的标记物，以及作为药物基因组学中的标记物。
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公开(公告)号：US06372432B1
公开(公告)日：2002-04-16
申请号：US09456461
申请日：1999-12-08
IPC分类号： C12Q168
CPC分类号： G01N33/56972 , C12Q1/6809
摘要： The present invention concerns new compositions and methods for the detection of pathological events. It more specifically concerns methods for the detection in vitro of the presence of a pathology or a pathological event in a subject, comprising taking a sample of blood cells from the subject and determining, in this sample, the presence of blood cells presenting a physiological state characteristic of the pathology. The invention also concerns the tools, kits and compositions for the implementation of such methods, as well as their uses in the field of human and animal health, or in experimental research for example.
摘要翻译： 本发明涉及用于检测病理事件的新组合物和方法。 更具体地涉及在体外检测受试者中病理学或病理学事件的存在的方法，其包括从受试者中取出血细胞样品，并在该样品中确定呈现生理状态的血细胞的存在 病理特征。 本发明还涉及用于实施这些方法的工具，试剂盒和组合物，以及它们在人类和动物健康领域或在实验研究领域中的用途。
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9.发明申请METHODS UTILIZING DIFFERENTIAL SPLICING EVENTS IN BLOOD CELLS FOR THE DETECTION OF PATHOLOGICAL EVENTS 审中-公开方法利用血液细胞中的差异性分离事件检测病理活动公开(公告)号：US20090264301A1
公开(公告)日：2009-10-22
申请号：US12494039
申请日：2009-06-29
IPC分类号： C40B30/00
CPC分类号： G01N33/56972 , C12Q1/6809
摘要： The present invention concerns new compositions and methods for the detection of pathological events. It more specifically concerns methods for the detection in vitro of the presence of a pathology or a pathological event in a subject, comprising taking a sample of blood cells from the subject and determining, in this sample, the presence of blood cells presenting a physiological state characteristic of the pathology. The invention also concerns the tools, kits and compositions for the implementation of such methods, as well as their uses in the field of human and animal health, or in experimental research for example.
摘要翻译： 本发明涉及用于检测病理事件的新组合物和方法。 更具体地涉及在体外检测受试者中病理学或病理学事件的存在的方法，其包括从受试者中取出血细胞样品，并在该样品中确定呈现生理状态的血细胞的存在 病理特征。 本发明还涉及用于实施这些方法的工具，试剂盒和组合物，以及它们在人类和动物健康领域或在实验研究领域中的用途。
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公开(公告)号：US06509153B1
公开(公告)日：2003-01-21
申请号：US09456370
申请日：1999-12-08
IPC分类号： C12Q168
CPC分类号： C12Q1/6809 , C12N15/1034 , C12Q1/6837
摘要： The present invention describes new methods for the determination of the potential toxicity of test compounds, as well as the kits and tools for the implementation of these methods. The invention also describes methods for generating nucleic acid sequences that can be used as genetic markers of toxicity. The invention is based in particular on the creation of differential nucleic acid banks characteristic of situations in which cell viability and/or proliferation are deregulated, and on the demonstration that these banks can be used to evaluate the toxicity profile of compounds with reliability and high sensitivity. The invention is of special utility in the pharmaceutical industry for analysis of the toxicity profile of compounds involved in drug development and/or in pharmaceutical compositions.
摘要翻译： 本发明描述了用于确定测试化合物的潜在毒性的新方法，以及用于实施这些方法的试剂盒和工具。 本发明还描述了用于产生可用作毒性遗传标记物的核酸序列的方法。 本发明特别地基于创造细胞活力和/或增殖失调的情况特征的差异核酸库，并且证明这些银行可以用于评估具有可靠性和高灵敏度的化合物的毒性特征 。 本发明在制药工业中用于分析涉及药物开发和/或药物组合物的化合物的毒性特征。
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