公开(公告)号：US07770437B2

公开(公告)日：2010-08-10

申请号：US11637390

申请日：2006-12-12

申请人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

发明人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

IPC分类号： G01N37/00

CPC分类号： G01N33/54353 ,  B05D1/185 ,  B82Y5/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08G65/329 ,  C08G65/334 ,  G01N33/54373 ,  G01N2610/00 ,  Y10T29/49002 ,  Y10T428/24

摘要： An article is provided for immobilizing functional organic biomolecules (e.g. proteins, DNA, and the like) through a covalent bond to a thiolate or disulfide monolayer to a metal surface wherein an extra activation step of the surface layer or an activation step of the functional biomolecules or bioreceptors could be avoided. The article comprises mixed self-assembled monolayers of thiol or disulfide molecules of formula X1—(CH2)c—O—([CH2]t—CH2—O)n—R1—S—X2 incorporating poly(ethylene oxide) groups and two functional groups, X1 and X2. Preferably, one functional group resists nonspecific adsorption and the other functional group directly (without activation) reacts with functional groups on the biomolecules. The functional group X1 is selected from the group consisting of flurophenyl, fluorobenzoyl, fluorophenoxycarbonyl, nitrophenoxycarbonyl, C2-12 alkenyl, sulfonyl halide, isothiocyanato, isocyanato, carbonyl halide, haloalkylcarbonyl, and diazonium carbonyl. The poly(ethylene oxide) groups resist nonspecific adsorption and enhance the specific affinity interactions. A particularly preferred molecule is 2-(2-{2-[2-(2-{2-[2-(11-mercaptoyldisulfanyl-undecyloxy)ethoxy]ethoxy}ethoxy)ethoxy]ethoxy} acetic acid pentafluorophenyl ester. A sensor device including these monolayers is also provided to perform reproducible, sensitive, specific and stable bioanalysis.

摘要翻译： 提供了一种用于将功能性有机生物分子（例如蛋白质，DNA等）通过共价键固定到硫醇盐或二硫化物单层到金属表面的物品，其中表面层的额外活化步骤或功能性生物分子的活化步骤 或生物受体可以避免。 该制品包括式X1-（CH2）c-O - （[CH2] t-CH2-O）n-R1-S-X2的结合聚（环氧乙烷）基团的二硫或二硫化物分子的混合自组装单层和两 官能团，X1和X2。 优选地，一个官能团抵抗非特异性吸附，另一个官能团直接（无活化）与生物分子上的官能团反应。 官能团X1选自氟苯基，氟苯甲酰基，氟苯氧基羰基，硝基苯氧基羰基，C2-12烯基，磺酰卤，异硫氰酸酯，异氰酸酯基，羰基卤，卤代烷基羰基和重氮基羰基。 聚（环氧乙烷）基团抵抗非特异性吸附并增强特异性亲和力相互作用。 特别优选的分子是2-（2- {2- [2-（2- {2- [2-（11-巯基二硫烷基 - 十一烷氧基）乙氧基]乙氧基}乙氧基）乙氧基]乙氧基}乙酸五氟苯酯。 还提供了这些单层以进行可重复，敏感，特异和稳定的生物分析。

公开(公告)号：US20070272003A1

公开(公告)日：2007-11-29

申请号：US11637390

申请日：2006-12-12

申请人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

发明人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

IPC分类号： G01N37/00 ,  B05D1/00 ,  B32B7/00 ,  C07C291/00 ,  C07C381/00 ,  C07C61/00 ,  C07C61/40 ,  C07D303/02

CPC分类号： G01N33/54353 ,  B05D1/185 ,  B82Y5/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08G65/329 ,  C08G65/334 ,  G01N33/54373 ,  G01N2610/00 ,  Y10T29/49002 ,  Y10T428/24

摘要： An article is provided for immobilizing functional organic biomolecules (e.g. proteins, DNA, and the like) through a covalent bond to a thiolate or disulfide monolayer to a metal surface wherein an extra activation step of the surface layer or an activation step of the functional biomolecules or bioreceptors could be avoided. The monolayer can contain, but is not limited to, two moieties. One has a group that resists nonspecific adsorption and another has a group that directly (without activation) reacts with functional groups on the biomolecules. In addition, poly(ethylene oxide) groups are incorporated in the monolayer surfaces to resist the nonspecific adsorption and to enhance the specific affinity interactions. A sensor device including these monolayers is also provided to perform reproducible, sensitive, specific and stable bioanalysis.

摘要翻译： 提供了一种用于将功能性有机生物分子（例如蛋白质，DNA等）通过共价键固定到硫醇盐或二硫化物单层到金属表面的物品，其中表面层的额外活化步骤或功能性生物分子的活化步骤 或生物受体可以避免。 单层可以包含但不限于两个部分。 一个具有抵抗非特异性吸附的基团，另一个具有直接（不活化）与生物分子上的官能团反应的基团。 此外，聚（环氧乙烷）基团并入单层表面以抵抗非特异性吸附并增强特异性亲和力相互作用。 还提供了包括这些单层的传感器装置，以执行可重复，灵敏，特异和稳定的生物分析。

公开(公告)号：US08304256B2

公开(公告)日：2012-11-06

申请号：US13280133

申请日：2011-10-24

申请人： Filip Frederix ,  Kristien Bonroy

发明人： Filip Frederix ,  Kristien Bonroy

IPC分类号： G01N33/543

CPC分类号： G01N33/54346 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  G01N21/554 ,  G01N21/648 ,  G01N2021/258

摘要： In one aspect, this disclosure provides a substrate for determining the concentration of an analyte within a sample. The substrate includes a conductive region and a recognition layer, the conductive region including at least one particle and having a first surface operatively coupled with the recognition layer, the recognition layer comprising at least one recognition molecule. The distance between the first surface of the conductive region and the recognition molecule is selected such that when the analyte is bound to the recognition layer the combination of the at least one particle and the analyte exhibits at least one of the following effects when radiation is directed through the conductive region and the recognition layer: (i) a particle plasmon effect, (ii) a particle bulk interband absorption, (iii) analyte molecular absorption, and (iv) absorption by the analyte-particle combination.

摘要翻译： 在一方面，本公开提供了用于确定样品中分析物浓度的底物。 衬底包括导电区域和识别层，该导电区域包括至少一个颗粒并且具有与识别层可操作地耦合的第一表面，识别层包括至少一个识别分子。 选择导电区域的第一表面和识别分子之间的距离，使得当分析物结合到识别层时，当辐射被定向时，至少一个颗粒和分析物的组合显示以下效应中的至少一个 通过导电区域和识别层：（i）颗粒等离子体效应，（ii）颗粒体间带吸收，（iii）分析物分子吸收和（iv）被分析物 - 颗粒组合的吸收。

公开(公告)号：US20120094317A1

公开(公告)日：2012-04-19

申请号：US13280133

申请日：2011-10-24

申请人： Filip Frederix ,  Kristien Bonroy

发明人： Filip Frederix ,  Kristien Bonroy

IPC分类号： G01N21/75 ,  C12M1/40

CPC分类号： G01N33/54346 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  G01N21/554 ,  G01N21/648 ,  G01N2021/258

摘要： In one aspect, this disclosure provides a substrate for determining the concentration of an analyte within a sample. The substrate includes a conductive region and a recognition layer, the conductive region including at least one particle and having a first surface operatively coupled with the recognition layer, the recognition layer comprising at least one recognition molecule. The distance between the first surface of the conductive region and the recognition molecule is selected such that when the analyte is bound to the recognition layer the combination of the at least one particle and the analyte exhibits at least one of the following effects when radiation is directed through the conductive region and the recognition layer: (i) a particle plasmon effect, (ii) a particle bulk interband absorption, (iii) analyte molecular absorption, and (iv) absorption by the analyte-particle combination.

摘要翻译： 在一方面，本公开提供了用于确定样品中分析物浓度的底物。 衬底包括导电区域和识别层，该导电区域包括至少一个颗粒并且具有与识别层可操作地耦合的第一表面，识别层包括至少一个识别分子。 选择导电区域的第一表面和识别分子之间的距离，使得当分析物结合到识别层时，当辐射被定向时，至少一个颗粒和分析物的组合显示以下效应中的至少一个 通过导电区域和识别层：（i）颗粒等离子体效应，（ii）颗粒体间带吸收，（iii）分析物分子吸收和（iv）被分析物 - 颗粒组合的吸收。

公开(公告)号：US20070117151A1

公开(公告)日：2007-05-24

申请号：US11555544

申请日：2006-11-01

申请人： Filip Frederix ,  Kristien Bonroy

发明人： Filip Frederix ,  Kristien Bonroy

IPC分类号： G01N33/53

CPC分类号： G01N33/54346 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  G01N21/554 ,  G01N21/648 ,  G01N2021/258

摘要： A method for determining a concentration of an analyte is provided. The method includes providing a substrate including a conductive region and a recognition layer where the conductive region has a first surface operatively coupled with the recognition layer; The method also includes contacting the substrate with the sample to bind at least some of the analyte that may present in the sample with the recognition layer; The method further includes directing radiation through the conductive region and the recognition layer, where the conductive region comprises at least one particle and a combination of the at least one particle effect when the radiation is directed through the conductive region and the recognition layer; The method still further includes measuring at least a part of a spectrum of the radiation that is absorbed or transmitted by or through the substrate, the at least part of the spectrum being related to one or more of the at least one particle effects; The method further includes determining a change of the at least part of the spectrum as compared with a reference spectrum and determining the concentration of the analyte from the change.

摘要翻译： 提供了一种用于确定分析物浓度的方法。 该方法包括提供包括导电区域和识别层的衬底，其中导电区域具有可操作地与识别层耦合的第一表面; 该方法还包括使基底与样品接触以将可能存在于样品中的至少一些分析物与识别层结合; 该方法还包括引导辐射穿过导电区域和识别层，其中当辐射被引导通过导电区域和识别层时，导电区域包括至少一个颗粒和至少一个颗粒效应的组合; 该方法还包括测量被衬底或通过衬底吸收或透过的辐射光谱的至少一部分，该光谱的至少部分与至少一种或多种颗粒效应有关; 该方法还包括与参考光谱相比确定光谱的至少部分的变化，并确定来自变化的分析物的浓度。

公开(公告)号：US08142720B2

公开(公告)日：2012-03-27

申请号：US12841800

申请日：2010-07-22

申请人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

发明人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC分类号： G01N33/54353 ,  B05D1/185 ,  B82Y5/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08G65/329 ,  C08G65/334 ,  G01N33/54373 ,  G01N2610/00 ,  Y10T29/49002 ,  Y10T428/24

摘要： An article is provided for immobilizing functional organic biomolecules (e.g. proteins, DNA, and the like) through a covalent bond to a thiolate or disulfide monolayer to a metal surface wherein an extra activation step of the surface layer or an activation step of the functional biomolecules or bioreceptors could be avoided. The monolayer can contain, but is not limited to, two moieties. One has a group that resists nonspecific adsorption and another has a group that directly (without activation) reacts with functional groups on the biomolecules. In addition, poly(ethylene oxide) groups are incorporated in the monolayer surfaces to resist the nonspecific adsorption and to enhance the specific affinity interactions. A sensor device including these monolayers is also provided to perform reproducible, sensitive, specific and stable bioanalysis.

摘要翻译： 提供了一种用于将功能性有机生物分子（例如蛋白质，DNA等）通过共价键固定到硫醇盐或二硫化物单层到金属表面的物品，其中表面层的额外活化步骤或功能性生物分子的活化步骤 或生物受体可以避免。 单层可以包含但不限于两个部分。 一个具有抵抗非特异性吸附的基团，另一个具有直接（不活化）与生物分子上的官能团反应的基团。 此外，聚（环氧乙烷）基团并入单层表面以抵抗非特异性吸附并增强特异性亲和力相互作用。 还提供了包括这些单层的传感器装置，以执行可重复，灵敏，特异和稳定的生物分析。

公开(公告)号：US08043868B2

公开(公告)日：2011-10-25

申请号：US11555544

申请日：2006-11-01

申请人： Filip Frederix ,  Kristien Bonroy

发明人： Filip Frederix ,  Kristien Bonroy

IPC分类号： G01N33/543

CPC分类号： G01N33/54346 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  G01N21/554 ,  G01N21/648 ,  G01N2021/258

摘要： A method for determining a concentration of an analyte is provided. The method includes providing a substrate including a conductive region and a recognition layer where the conductive region has a first surface operatively coupled with the recognition layer; The method also includes contacting the substrate with the sample to bind at least some of the analyte that may present in the sample with the recognition layer; The method further includes directing radiation through the conductive region and the recognition layer, where the conductive region comprises at least one particle and a combination of the at least one particle effect when the radiation is directed through the conductive region and the recognition layer; The method still further includes measuring at least a part of a spectrum of the radiation that is absorbed or transmitted by or through the substrate, the at least part of the spectrum being related to one or more of the at least one particle effects; The method further includes determining a change of the at least part of the spectrum as compared with a reference spectrum and determining the concentration of the analyte from the change.

摘要翻译： 提供了一种用于确定分析物浓度的方法。 该方法包括提供包括导电区域和识别层的衬底，其中导电区域具有可操作地与识别层耦合的第一表面; 该方法还包括使基底与样品接触以将可能存在于样品中的至少一些分析物与识别层结合; 该方法还包括引导辐射穿过导电区域和识别层，其中当辐射被引导通过导电区域和识别层时，导电区域包括至少一个颗粒和至少一个颗粒效应的组合; 该方法还包括测量被衬底或通过衬底吸收或透过的辐射光谱的至少一部分，该光谱的至少部分与至少一种或多种颗粒效应有关; 该方法还包括与参考光谱相比确定光谱的至少部分的变化，并确定来自变化的分析物的浓度。

公开(公告)号：US20100284860A1

公开(公告)日：2010-11-11

申请号：US12841800

申请日：2010-07-22

申请人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

发明人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

IPC分类号： G01N33/00 ,  C07F1/08 ,  C07F3/10 ,  C07F7/24 ,  C07F15/02 ,  C07F11/00 ,  C07F13/00 ,  C07F5/06 ,  C07F1/10 ,  C07F1/12 ,  C07F3/08 ,  H05K13/00

CPC分类号： G01N33/54353 ,  B05D1/185 ,  B82Y5/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08G65/329 ,  C08G65/334 ,  G01N33/54373 ,  G01N2610/00 ,  Y10T29/49002 ,  Y10T428/24

摘要： An article is provided for immobilizing functional organic biomolecules (e.g. proteins, DNA, and the like) through a covalent bond to a thiolate or disulfide monolayer to a metal surface wherein an extra activation step of the surface layer or an activation step of the functional biomolecules or bioreceptors could be avoided. The monolayer can contain, but is not limited to, two moieties. One has a group that resists nonspecific adsorption and another has a group that directly (without activation) reacts with functional groups on the biomolecules. In addition, poly(ethylene oxide) groups are incorporated in the monolayer surfaces to resist the nonspecific adsorption and to enhance the specific affinity interactions. A sensor device including these monolayers is also provided to perform reproducible, sensitive, specific and stable bioanalysis.

摘要翻译： 提供了一种用于将功能性有机生物分子（例如蛋白质，DNA等）通过共价键固定到硫醇盐或二硫化物单层到金属表面的物品，其中表面层的额外活化步骤或功能性生物分子的活化步骤 或生物受体可以避免。 单层可以包含但不限于两个部分。 一个具有抵抗非特异性吸附的基团，另一个具有直接（不活化）与生物分子上的官能团反应的基团。 此外，聚（环氧乙烷）基团并入单层表面以抵抗非特异性吸附并增强特异性亲和力相互作用。 还提供了包括这些单层的传感器装置，以执行可重复，灵敏，特异和稳定的生物分析。

公开(公告)号：US20110040381A1

公开(公告)日：2011-02-17

申请号：US12741681

申请日：2008-11-06

申请人： Timothy James Kidd ,  Robert Hendrik Catharina Janssen ,  Filip Frederix ,  Jan Stolk ,  Harold Jan Smelt

发明人： Timothy James Kidd ,  Robert Hendrik Catharina Janssen ,  Filip Frederix ,  Jan Stolk ,  Harold Jan Smelt

IPC分类号： A61F2/44 ,  B29C45/00 ,  A61F2/32 ,  A61F2/40 ,  A61F2/38 ,  C08F136/20 ,  C08J3/28

CPC分类号： C08F210/02 ,  A61L27/16 ,  C08L23/06 ,  C08F236/20 ,  C08F2500/01 ,  C08F2500/14 ,  C08F236/06

摘要： The invention relates to a process for the production of an ultra-high molecular weight polyethylene ((U)HMWPE) article comprising: —copolymerizing ethylene with a linear, branched or cyclic polyene having 3 to 100 carbon atoms, resulting in a copolymer of ethylene and polyene ((U)HMWPE-P), using such a content of polyene that the number of polyene branches in (U)HMWPE-P is 0.01 to 15 on the average per 1000 carbon atoms; —cross-link the (U)HMWPE-P during or after molding the (U)HMWPE-P.

摘要翻译： 本发明涉及一种制备超高分子量聚乙烯（（U）HMWPE）制品的方法，其包括：将具有3至100个碳原子的直链，支链或环状多烯的乙烯聚合，得到乙烯的共聚物 和（多）烯（（U）HMWPE-P）），使用这样的多烯含量，（U）HMWPE-P中的多烯分支数为每1000个碳原子平均为0.01至15; 在（U）HMWPE-P成型期间或之后 - （U）HMWPE-P交联。

公开(公告)号：US20080131869A1

公开(公告)日：2008-06-05

申请号：US11769237

申请日：2007-06-27

申请人： Filip Frederix ,  Gustaaf Borghs ,  Jean-Michel Friedt

发明人： Filip Frederix ,  Gustaaf Borghs ,  Jean-Michel Friedt

IPC分类号： C12Q1/70 ,  G01N21/62 ,  G01N33/53 ,  C12Q1/68

CPC分类号： G01N21/554 ,  B82Y30/00 ,  G01N21/77 ,  G01N33/54373 ,  G01N2021/258 ,  G01N2021/7783

摘要： The present invention discloses an improved method for detecting an analyte. The present invention may be used for sensing devices which have a higher sensitivity and which can be used to detect very low concentration of analyte. In one embodiment, the method comprises the steps of providing a substrate, said substrate comprising a conductive region and a recognition layer, said conductive region having at least a first surface and a second surface, wherein said first surface is operatively associated with said recognition layer; subjecting said substrate to said analyte such that an interaction occurs between said analyte and said recognition layer; directing radiation through said substrate such that said radiation incidents on said conductive region and said recognition layer; and measuring the intensity of said radiation absorbed or transmitted by said substrate as a function of the wavelength in order to determine the presence of an analyte.

摘要翻译： 本发明公开了一种用于检测分析物的改进方法。 本发明可以用于具有较高灵敏度并可用于检测非常低浓度分析物的感测装置。 在一个实施例中，该方法包括以下步骤：提供衬底，所述衬底包括导电区域和识别层，所述导电区域具有至少第一表面和第二表面，其中所述第一表面与所述识别层可操作地相关联 ; 使所述衬底经受所述分析物，使得所述分析物与所述识别层之间发生相互作用; 引导辐射穿过所述衬底，使得所述辐射突发在所述导电区域和所述识别层上; 并测量由所述基底吸收或透射的所述辐射的强度作为波长的函数，以便确定分析物的存在。