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公开(公告)号：US11293040B2
公开(公告)日：2022-04-05
申请号：US16308890
申请日：2017-07-19
申请人： Firmenich SA
发明人： Michel Schalk , Letizia Rocci
摘要： Provided is a method of producing one or more sesquiterpene compounds comprising: contacting an acyclic FPP precursor with a polypeptide having terpene synthase activity, wherein the polypeptide comprises an amino acid sequence that has at least 55% sequence identity to SEQ ID NO: 1, to produce one or more terpenes selected from the group consisting of isovalencene, spirovetiva-1(10),7(11)-diene and valencene or derivatives thereof, or mixture of sesquiterpenes comprising one or more of isovalencene, spirovetiva-1(10),7(11)-diene and/or valencene; and optionally isolating the one or more terpenes or the mixture. Also described is a nucleic acid derived from Vetiveria zizanoides encoding a polypeptide having sesquiterpene synthase activity, a polypeptide that can be used to produce one or more sesquiterpenes or a mixture of sesquiterpenes comprising one or more of isovalencene, spirovetiva-1(10),7(11)-diene and/or valencene, and a non-human organism or cell comprising the nucleic acid or comprising an expression vector comprising the nucleic acid.
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公开(公告)号：US20230151393A1
公开(公告)日：2023-05-18
申请号：US16766225
申请日：2019-01-16
申请人： FIRMENICH SA
发明人： Michel Schalk , Letizia Rocci , Fabienne Deguerry
CPC分类号： C12P7/02 , C12N5/10 , C12N15/63 , C12N15/52 , C12N9/0077 , C12Y114/15006 , C12P5/007
摘要： Described herein are the nucleic acid and the amino acid sequences of a cytochrome P450 capable of oxidizing terpene molecules. Also described herein are methods of oxidizing terpene molecules including contacting the cytochrome P450 with the terpene molecule intended to be oxidized. In particular, the method may be carried out in vitro or in vivo to produce oxidized terpene molecules, which may be used in different technical fields such as for example perfumery and flavoring. Also described herein is an expression vector containing the nucleic acid. A non-human host organism or a cell transformed with the nucleic acid is also an object of the disclosure.
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公开(公告)号：US11214775B2
公开(公告)日：2022-01-04
申请号：US15734887
申请日：2019-07-10
申请人： FIRMENICH SA
摘要： Provided herein are biocatalytic methods of producing terpene compounds by applying a novel type of phosphatase enzyme. The method allows the fully biochemical synthesis of terpene compounds, like for example copalol and labdendiol, and derivatives thereof, which serve as valuable intermediates for the production of perfumery ingredients, such as, for example, ambrox. Also provided are novel fully biochemical multistep processes for the production of such compounds as well as novel phosphatase enzymes and mutants and variants derived therefrom.
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公开(公告)号：US20210010035A1
公开(公告)日：2021-01-14
申请号：US16938605
申请日：2020-07-24
申请人： FIRMENICH SA
摘要： Described herein are methods of producing (+)-manool, the methods including: contacting geranylgeranyl diphosphate with a copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate and contacting the CPP with a sclareol synthase enzyme to form (+)-manool and derivatives thereof. Also described herein are nucleic acids encoding CPP synthases and sclareol synthases for use in the methods. Further described herein are expression vectors and non-human host organisms and cells including nucleic acids encoding a CPP synthase and a sclareol synthase as described herein.
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公开(公告)号：US20200172936A1
公开(公告)日：2020-06-04
申请号：US16738563
申请日：2020-01-09
申请人： Firmenich SA
发明人： Laurent Daviet , Letizia Rocci , Michel Schalk
摘要： This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a P450 polypeptide with monooxygenase activity.
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公开(公告)号：US20220056485A1
公开(公告)日：2022-02-24
申请号：US17463830
申请日：2021-09-01
申请人： Firmenich SA
发明人： Laurent Daviet , Letizia Rocci , Michel Schalk
摘要： This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a P450 polypeptide with monooxygenase activity.
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公开(公告)号：US11130972B2
公开(公告)日：2021-09-28
申请号：US16738563
申请日：2020-01-09
申请人： Firmenich SA
发明人： Laurent Daviet , Letizia Rocci , Michel Schalk
摘要： This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a P450 polypeptide with monooxygenase activity.
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公开(公告)号：US10570420B2
公开(公告)日：2020-02-25
申请号：US15877183
申请日：2018-01-22
申请人： FIRMENICH SA
发明人： Laurent Daviet , Letizia Rocci , Michel Schalk
摘要： This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a P450 polypeptide with monooxygenase activity.
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公开(公告)号：US09909145B2
公开(公告)日：2018-03-06
申请号：US15023640
申请日：2014-09-19
申请人： FIRMENICH SA
发明人： Laurent Daviet , Letizia Rocci , Michel Schalk
IPC分类号： C12N9/02 , C12P21/06 , C12P19/34 , C12P7/02 , C12P7/04 , C12N1/20 , C12N1/00 , C12N5/00 , C07H21/04 , C12N15/82 , C07K1/00 , C12P5/00
CPC分类号： C12P5/007 , C12N9/0071 , C12N9/0079 , C12P7/02 , C12Y114/13 , C12Y114/14001
摘要： This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a p450 polypeptide with monooxygenase activity.
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公开(公告)号：US20210222138A1
公开(公告)日：2021-07-22
申请号：US15734887
申请日：2019-07-10
申请人： FIRMENICH SA
摘要： Provided herein are biocatalytic methods of producing terpene compounds by applying a novel type of phosphatase enzyme. The method allows the fully biochemical synthesis of terpene compounds, like for example copalol and labdendiol, and derivatives thereof, which serve as valuable intermediates for the production of perfumery ingredients, such as, for example, ambrox. Also provided are novel fully biochemical multistep processes for the production of such compounds as well as novel phosphatase enzymes and mutants and variants derived therefrom.
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