公开(公告)号：US20210356376A1

公开(公告)日：2021-11-18

申请号：US17274252

申请日：2019-09-09

申请人： Fluidigm Canada Inc. ,  The Governing Council of the University of Toronto

发明人： Taunia Closson ,  Vladimir Baranov ,  Mitchell A. Winnik

IPC分类号： G01N15/10 ,  G01N33/68 ,  H01J49/00

摘要： This disclosure relates to reagents and their use for elemental imaging mass spectrometry of biological samples.

公开(公告)号：US10669358B2

公开(公告)日：2020-06-02

申请号：US16047927

申请日：2018-07-27

申请人： FLUIDIGM CANADA INC.

发明人： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC分类号： G01N33/532 ,  C08F8/42 ,  C08F8/32 ,  C08F220/36 ,  G01N33/58 ,  C08F8/14 ,  C08F220/56 ,  G01N33/574

摘要： Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US10072104B2

公开(公告)日：2018-09-11

申请号：US14875553

申请日：2015-10-05

申请人： FLUIDIGM CANADA INC.

发明人： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC分类号： G01N33/574 ,  C08F220/56 ,  C08F8/32 ,  C08F8/42 ,  C08F220/36 ,  G01N33/532 ,  G01N33/58 ,  C08F8/14

CPC分类号： C08F8/32 ,  C08F8/14 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

摘要： Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US12103996B2

公开(公告)日：2024-10-01

申请号：US16867023

申请日：2020-05-05

申请人： Fluidigm Canada Inc.

发明人： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC分类号： G01N33/532 ,  C08F8/14 ,  C08F8/32 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/574 ,  G01N33/58

CPC分类号： C08F220/36 ,  C08F8/14 ,  C08F8/32 ,  C08F8/42 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

摘要： Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US20160370277A1

公开(公告)日：2016-12-22

申请号：US15253060

申请日：2016-08-31

申请人： Fluidigm Canada Inc.

发明人： Mitchell A. Winnik ,  Cedric Van-Caeyzeele ,  Vladimir I. Baranov

IPC分类号： G01N15/10 ,  H01J49/00 ,  G01N33/553 ,  G01N33/68 ,  G01N33/543

CPC分类号： G01N15/1012 ,  G01N33/54313 ,  G01N33/54353 ,  G01N33/553 ,  G01N33/6848 ,  H01J49/0009 ,  Y10T436/24

摘要： The invention relates to a new type of element encoded particles suitable for the attachment of bio molecules to enable massively multiplex bio-analytical methods, and to calibrate and tune the elemental flow cytometer mass spectrometer (FC-MS) (FIG. 1).

摘要翻译： 本发明涉及适用于附着生物分子以实现大规模多重生物分析方法以及校准和调整元流式细胞仪质谱仪（FC-MS）（图1）的新型元素编码粒子。

公开(公告)号：US09296838B2

公开(公告)日：2016-03-29

申请号：US14659224

申请日：2015-03-16

申请人： FLUIDIGM CANADA INC.

发明人： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC分类号： G01N33/544 ,  G01N33/545 ,  G01N33/24 ,  C08F8/32 ,  C08F8/42 ,  C08F220/36 ,  G01N33/532 ,  G01N33/58 ,  C08F8/14

CPC分类号： C08F8/32 ,  C08F8/14 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

摘要： Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US10752707B2

公开(公告)日：2020-08-25

申请号：US16506243

申请日：2019-07-09

申请人： Fluidigm Canada Inc.

发明人： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC分类号： G01N33/532 ,  C08F8/42 ,  C08F8/32 ,  C08F220/36 ,  G01N33/58 ,  C08F8/14 ,  C08F220/56 ,  G01N33/574

摘要： Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US09169335B2

公开(公告)日：2015-10-27

申请号：US14659224

申请日：2015-03-16

申请人： FLUIDIGM CANADA INC.

发明人： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC分类号： G01N33/544 ,  G01N33/545 ,  G01N33/24 ,  C08F8/32 ,  C08F8/14 ,  C08F8/42

摘要： Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US20150183895A1

公开(公告)日：2015-07-02

申请号：US14659224

申请日：2015-03-16

申请人： FLUIDIGM CANADA INC.

发明人： Mitchell A. Winnik ,  MARK NITZ ,  VLADIMIR BARANOV ,  XUDONG LOU

IPC分类号： C08F8/32 ,  C08F8/42 ,  C08F8/14

CPC分类号： C08F8/32 ,  C08F8/14 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

摘要： Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

摘要翻译： 提供基于新型金属 - 聚合物共轭物的元件标签用于元素分析，包括ICP-MS。 聚合物主链被官能化以不可逆地结合在用户使用之前选择的金属。 该聚合物进一步官能化以连接允许附着于抗体或其它亲和试剂的接头。 聚合物形式允许附加许多拷贝的给定同位素，其线性地提高灵敏度。 金属 - 聚合物缀合物标签使得能够以两种形式进行多重测定：批量测定，其中样品中的平均生物标志物分布是诊断的，以及单细胞格式以区分复杂样品中的罕见（例如患病）细胞（例如， 血液）。