-
公开(公告)号：US08921049B2
公开(公告)日：2014-12-30
申请号：US13869804
申请日：2013-04-24
Applicant: Fluidigm Corporation
Inventor： Simant Dube , Alain Mir , Ramesh Ramakrishnan , Lesley Suzanne Weaver , Bernhard G. Zimmermann
CPC classification number: C12Q1/6853 , C12Q1/6809 , C12Q1/6886 , C12Q2600/16 , C12Q2561/113 , C12Q2545/10 , C12Q2527/107
Abstract: The present invention provides for determining relative copy number difference for one or more target nucleic acid sequences between a test sample and a reference sample or reference value derived therefrom. The methods facilitate the detection of copy number differences less than 1.5-fold.
Abstract translation: 本发明提供了确定测试样品和参考样品之间的一个或多个目标核酸序列的相对拷贝数差异或其衍生的参考值。 该方法有助于检测小于1.5倍的拷贝数差异。
-
公开(公告)号：US20140154679A1
公开(公告)日：2014-06-05
申请号：US13869804
申请日：2013-04-24
Applicant: Fluidigm Corporation
Inventor： Simant Dube , Alain Mir , Ramesh Ramakrishnan , Lesley Suzanne Weaver , Bernhard G. Zimmermann
IPC: C12Q1/68
CPC classification number: C12Q1/6853 , C12Q1/6809 , C12Q1/6886 , C12Q2600/16 , C12Q2561/113 , C12Q2545/10 , C12Q2527/107
Abstract: The present invention provides for determining relative copy number difference for one or more target nucleic acid sequences between a test sample and a reference sample or reference value derived therefrom. The methods facilitate the detection of copy number differences less than 1.5-fold.
Abstract translation: 本发明提供了确定测试样品和参考样品之间的一个或多个目标核酸序列的相对拷贝数差异或其衍生的参考值。 该方法有助于检测小于1.5倍的拷贝数差异。
-
公开(公告)号：US08849037B2
公开(公告)日：2014-09-30
申请号：US14071423
申请日：2013-11-04
Applicant: Fluidigm Corporation
Inventor： Simant Dube , Gang Sun , Lian-She Zhao
CPC classification number: G01N21/64 , G06T7/0012 , G06T7/74 , G06T2207/10064 , G06T2207/30004
Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.
Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。
-
公开(公告)号：US20140133732A1
公开(公告)日：2014-05-15
申请号：US14071423
申请日：2013-11-04
Applicant: Fluidigm Corporation
Inventor： Simant Dube , Gang Sun , Lian-She Zhao
IPC: G01N21/64
CPC classification number: G01N21/64 , G06T7/0012 , G06T7/74 , G06T2207/10064 , G06T2207/30004
Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.
Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。
-
-
-
Search Results
4
records
(took 0.015 seconds)
