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公开(公告)号：US09074251B2
公开(公告)日：2015-07-07
申请号：US13080345
申请日：2011-04-05
申请人： Frank J. Steemers , Kevin Gunderson , Thomas Royce , Natasha Pignatelli , Igor Yu Goryshin , Nicholas Caruccio
发明人： Frank J. Steemers , Kevin Gunderson , Thomas Royce , Natasha Pignatelli , Igor Yu Goryshin , Nicholas Caruccio
IPC分类号： C12Q1/68
CPC分类号： C12N15/1082 , C12Q1/6869 , C12Q1/6874 , C12Q2521/319 , C12Q2521/507 , C12Q2525/155 , C12Q2525/161 , C12Q2525/185 , C12Q2525/191 , C12Q2525/197 , C12Q2525/313 , C12Q2535/122 , C12Q2565/514 , C12Q2521/301 , C12Q2521/327
摘要： Artificial transposon sequences having code tags and target nucleic acids containing such sequences. Methods for making artificial transposons and for using their properties to analyze target nucleic acids.
摘要翻译： 具有编码标签的人工转座子序列和含有该序列的靶核酸。 制备人工转座子和使用其性质分析靶核酸的方法。
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公开(公告)号：US20120208724A1
公开(公告)日：2012-08-16
申请号：US13080345
申请日：2011-04-05
申请人： Frank J. Steemers , Kevin Gunderson , Thomas Royce , Natasha Pignatelli , Igor Yu Goryshin , Nicholas Caruccio
发明人： Frank J. Steemers , Kevin Gunderson , Thomas Royce , Natasha Pignatelli , Igor Yu Goryshin , Nicholas Caruccio
CPC分类号： C12N15/1082 , C12Q1/6869 , C12Q1/6874 , C12Q2521/319 , C12Q2521/507 , C12Q2525/155 , C12Q2525/161 , C12Q2525/185 , C12Q2525/191 , C12Q2525/197 , C12Q2525/313 , C12Q2535/122 , C12Q2565/514 , C12Q2521/301 , C12Q2521/327
摘要： Artificial transposon sequences having code tags and target nucleic acids containing such sequences. Methods for making artificial transposons and for using their properties to analyze target nucleic acids.
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公开(公告)号：US5965443A
公开(公告)日：1999-10-12
申请号：US814877
申请日：1996-09-09
CPC分类号： C12N15/85 , C12N15/90 , C12N9/22 , C12N2800/90
摘要： A system for in vitro transposition includes a donor DNA that includes a transposable element flanked by a pair of bacterial transposon Tn5 outside end repeat sequences, a target DNA into which the transposable element can transpose, and a modified Tn5 transposase having higher binding avidity to the outside end repeat sequences and being less likely to assume an inactive multimer form than wild type Tn5 transposase.
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公开(公告)号：US5948622A
公开(公告)日：1999-09-07
申请号：US944916
申请日：1997-10-06
IPC分类号： C12N15/09 , C12N9/22 , C12N15/55 , C12N15/85 , C12N15/90 , C12Q1/68 , C07H21/04 , C12N15/63 , C12N15/74
CPC分类号： C12N15/85 , C12N15/90 , C12N9/22 , C12N2800/90
摘要： A system for in vitro transposition includes a donor DNA that includes a transposable element flanked by a pair of bacterial transposon Tn5 outside end repeat sequences, a target DNA into which the transposable element can transpose, and a modified Tn5 transposase having higher binding avidity to the outside end repeat sequences and being less likely to assume an inactive multimer form than wild type Tn5 transposase.
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公开(公告)号：US07338801B2
公开(公告)日：2008-03-04
申请号：US11369497
申请日：2006-03-07
摘要： Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each pair of transposase-interacting sequences interacts with a distinct transposase enzyme.
摘要翻译： 提供了用于操纵核酸以产生基因融合体，删除或克隆染色体的一部分或将序列插入染色体的方法。 所述方法使用在转座多核苷酸上使用两对或多对反向重复转座酶相互作用序列的顺序转座过程，其中每对转座酶相互作用序列与不同的转座酶相互作用。
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公开(公告)号：US06437109B1
公开(公告)日：2002-08-20
申请号：US09272432
申请日：1999-03-19
IPC分类号： C07H2104
CPC分类号： C12N15/85 , C12N9/22 , C12N15/90 , C12N2800/90
摘要： A system for in vitro transposition includes a donor DNA that includes a transposable element flanked by a pair of bacterial transposon Tn5 outside end repeat sequences, a target DNA into which the transposable element can transpose, and a modified Tn5 transposase having higher binding avidity to the outside end repeat sequences and being less likely to assume an inactive multimer form than wild type Tn5 transposase.
摘要翻译： 用于体外转座的系统包括供体DNA，其包含侧转有一对细胞转座子Tn5外端重复序列的转座元件，可转座元件可转座的靶DNA，以及具有较高结合亲合力的修饰的Tn5转座酶 外端重复序列，并且与野生型Tn5转座酶相比不太可能呈现无活性多聚体形式。
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公开(公告)号：US07067644B2
公开(公告)日：2006-06-27
申请号：US10007483
申请日：2001-12-05
摘要： Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each pair of transposase-interacting sequences interacts with a distinct transposase enzyme.
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公开(公告)号：US5925545A
公开(公告)日：1999-07-20
申请号：US850880
申请日：1997-05-02
IPC分类号： C12N15/09 , C12N9/22 , C12N15/55 , C12N15/85 , C12N15/90 , C12P21/06 , C07H21/04 , C12N15/00 , C07K13/00
CPC分类号： C12N15/85 , C12N15/90 , C12N9/22 , C12N2800/90
摘要： A system for in vitro transposition includes a donor DNA that includes a transposable element flanked by a pair of bacterial transposon Tn5 outside end repeat sequences, a target DNA into which the transposable element can transpose, and a modified Tn5 transposase having higher binding avidity to the outside end repeat sequences and being less likely to assume an inactive multimer form than wild type Tn5 transposase.
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