公开(公告)号：US07280203B2

公开(公告)日：2007-10-09

申请号：US10924658

申请日：2004-08-24

Applicant: Frank Olschewski

Inventor： Frank Olschewski

IPC: G01J3/30 ,  F21V9/16

CPC classification number: G01N21/645 ,  G01J3/36 ,  G01J3/4406 ,  G01N2021/6417 ,  G02B21/0076

Abstract: A method for separating fluorescence spectra of dyes present in a specimen (15) is disclosed. Firstly a spectral scan of the fluorescence spectrum of all the dyes present in the specimen (15) is performed. The fluorescence spectra associated with the respective dyes are stored in a database of the computer system. After spectral deconvolution of the acquired mixed fluorescence spectrum, a comparison is made between the measured individual spectra ascertained by spectral deconvolution and the fluorescence spectra associated with the respective dyes. Lastly, a linear deconvolution of the acquired mixed fluorescence spectrum is performed.

Abstract translation: 公开了一种用于分离存在于样品（15）中的染料的荧光光谱的方法。 首先进行样品（15）中存在的所有染料的荧光光谱的光谱扫描。 与相应染料相关的荧光光谱存储在计算机系统的数据库中。 在获得的混合荧光光谱的光谱去卷积之后，通过光谱反卷积确定的测量的单个光谱和与各个染料相关的荧光光谱进行比较。 最后，进行采集的混合荧光光谱的线性去卷积。

公开(公告)号：US07394482B2

公开(公告)日：2008-07-01

申请号：US10694109

申请日：2003-10-27

Applicant: Frank Olschewski

Inventor： Frank Olschewski

IPC: H04N7/18 ,  H04N9/47

CPC classification number: G01J3/4406 ,  G02B21/002

Abstract: A microscope system and a method that record spectra (60a, 61a, 62a, 63a, and 66a) of the dyes present in the specimen (15) using an SP module (20) are disclosed. A transformation of the data of the ascertained spectra, and of the dye spectra (60b, 61b, 62b, 63b, and 66b) stored in a database, is performed. The spectra are entered into a correspondingly into a divided transformation space. Allocation of the dye spectra (60b, 61b, 62b, 63b, and 66b) to the measured spectra (60a, 61a, 62a, 63a, and 66a) is accomplished by way of a comparison in the transformation space.

Abstract translation: 公开了使用SP模块（20）记录样品（15）中存在的染料的光谱（60a，61a，62a，63a和66a）的显微镜系统和方法。 执行存储在数据库中的确定的光谱和染料光谱（60b，61b，62b，63b和66b）的数据的变换。 将光谱相应地输入到分割的变换空间中。 将染料光谱（60b，61b，62b，63b和66b）分配到测量光谱（60a，61a，62a，63a和66a）中，通过比较 在转型空间。

公开(公告)号：US07282724B2

公开(公告)日：2007-10-16

申请号：US10984627

申请日：2004-11-09

Applicant: Frank Olschewski

Inventor： Frank Olschewski

IPC: G01N21/64

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/1293

Abstract: A method and a system for the analysis of co-localizations of dyes present in a specimen. The fluorescence spectra of the dyes present in the specimen are determined. A tolerance region around each of the fluorescence spectra is selected. The spectra of the specimen, in which at least two dyes are present, are then acquired pixel by pixel. Those spectra that lie within the tolerance region around the fluorescence spectra are then calculated. A lambda vector is calculated for each pixel and assigned to a spectrum. Images can be displayed in accordance with the assignment to the spectra.

Abstract translation: 用于分析样品中存在的染料的共定位的方法和系统。 确定样品中存在的染料的荧光光谱。 选择每个荧光光谱周围的公差区域。 其中存在至少两种染料的样品的光谱随后逐个获得。 然后计算位于荧光光谱周围的容限区域内的那些光谱。 为每个像素计算λ矢量并分配给一个光谱。 可以根据对光谱的分配显示图像。

公开(公告)号：US20050109949A1

公开(公告)日：2005-05-26

申请号：US10984627

申请日：2004-11-09

Applicant: Frank Olschewski

Inventor： Frank Olschewski

IPC: G01N21/25 ,  G01N21/64 ,  G02B21/00 ,  H04N1/04

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/1293

Abstract: A method and a system for the analysis of co-localizations of dyes present in a specimen (15) are disclosed. For that purpose, the various fluorescence spectra of all dyes present in the specimen (15) are determined. A tolerance region around each of the fluorescence spectra is selected. The spectra of the specimen (15), in which at least two dyes are present, are then acquired pixel by pixel. Those spectra that lie within the tolerance region around the fluorescence spectra are then calculated. A lambda vector is calculated for each pixel and assigned to a spectrum. Images can be outputted on the display in accordance with the assignment to the spectra.

Abstract translation: 公开了一种用于分析存在于样品（15）中的染料的共定位的方法和系统。 为此，确定了样品（15）中存在的所有染料的各种荧光光谱。 选择每个荧光光谱周围的公差区域。 然后逐个像素地获取其中存在至少两种染料的样品（15）的光谱。 然后计算位于荧光光谱周围的容限区域内的那些光谱。 为每个像素计算λ矢量并分配给一个光谱。 可以根据对光谱的分配在显示器上输出图像。

公开(公告)号：US07257289B2

公开(公告)日：2007-08-14

申请号：US10250152

申请日：2003-06-07

Applicant: Frank Olschewski

Inventor： Frank Olschewski

IPC: G02B6/26

CPC classification number: G01J3/36 ,  G01J3/2823 ,  G02B21/002

Abstract: A spectral scanning microscope and a method for data acquisition using a spectral scanning microscope are disclosed. A computer system is provided that encompasses a memory and a database. In combination with the computer system and/or the database, a continuous wavelength subregion that serves to illuminate the specimen can be selected from a continuous wavelength region using the spectral selection means. Also in combination with the computer system together with the spectral selection means, a detection band can be selected from the detected light beam.

Abstract translation: 公开了一种使用光谱扫描显微镜的光谱扫描显微镜和数据采集方法。 提供了包括存储器和数据库的计算机系统。 与计算机系统和/或数据库组合，可以使用频谱选择装置从连续波长区域中选择用于照射样本的连续波长子区域。 另外，与计算机系统一起与频谱选择装置一起，可以从检测到的光束中选择检测频带。

公开(公告)号：US07221784B2

公开(公告)日：2007-05-22

申请号：US10604314

申请日：2003-07-10

Applicant: Frank Olschewski

Inventor： Frank Olschewski

IPC: G06K9/00

CPC classification number: G02B21/365

Abstract: A method and an arrangement for automatic three-dimensional recording of structures of interest in a sample (15) are disclosed. The arrangement possesses a microscope having at least one microscope objective (13). The images of a sample (15) are acquired using a detector unit (19). A computer system (23) controls acquisition of the images and the microscope functions. The computer system (23) possesses a means (25) for automatically recording the entire marked specimen region in three dimensions.

Abstract translation: 公开了一种用于样品（15）中感兴趣的结构的自动三维记录的方法和装置。 该装置具有至少一个显微镜物镜（13）的显微镜。 使用检测器单元（19）获取样品（15）的图像。 计算机系统（23）控制图像和显微镜功能的获取。 计算机系统（23）具有用于在三维中自动记录整个标记的标本区域的装置（25）。

公开(公告)号：US20070076232A1

公开(公告)日：2007-04-05

申请号：US10597555

申请日：2004-12-22

Applicant: Frank Olschewski ,  Jochen Nickel ,  William Hay

Inventor： Frank Olschewski ,  Jochen Nickel ,  William Hay

IPC: H04N1/60

CPC classification number: G02B21/365

Abstract: A microscope system includes at least one lens that defines an illumination field and at least one light source that emits an illuminating light beam for illuminating a specimen through the lens. At least one detector is provided for, pixel-by-pixel, detecting a detection light beam coming from the specimen. An electronic circuit is connected downstream from the detector, the electronic circuit including a memory unit for storing a wavelength-dependent brightness distribution of an illumination field of the at least one lens. The electronic circuit employs, pixel-by-pixel, the stored wavelength-dependent brightness distribution so as to form a homogeneously illuminated image field. An actuatable element is provided for controlling, pixel-by-pixel, an intensity of the illuminating light beam as a function of the stored wavelength-dependent brightness distribution so as to homogeneously illuminate the illumination field.

Abstract translation: 显微镜系统包括限定照明场的至少一个透镜和发射用于通过透镜照射样本的照明光束的至少一个光源。 提供至少一个检测器，用于逐像素地检测来自样品的检测光束。 电子电路连接在检测器的下游，电子电路包括用于存储至少一个透镜的照明场的波长相关亮度分布的存储单元。 电子电路逐像素地使用所存储的依赖于波长的亮度分布，以形成均匀照明的图像场。 提供可致动元件，用于逐个像素地控制作为所存储的依赖于波长的亮度分布的函数的照明光束的强度，以均匀地照射照明场。

公开(公告)号：US20050141081A1

公开(公告)日：2005-06-30

申请号：US11007736

申请日：2004-12-08

Applicant: Frank Olschewski

Inventor： Frank Olschewski

IPC: G02B21/26 ,  G02B21/36 ,  G02B27/64 ,  G06T7/20 ,  G01N23/00 ,  G02B21/00 ,  G21K7/00

CPC classification number: G02B27/646 ,  G02B21/26 ,  G02B21/367 ,  G06T7/238 ,  G06T2207/10056 ,  G06T2207/20104

Abstract: In an optical device (2), in particular a microscope, drift is sensed by the fact that a first image of an immovable specimen (30) is acquired at a first time (T(n-1)), and a second image thereof at a second time (T(n)). The drift is calculated from a comparison between the first and the second image.

Abstract translation: 在光学装置（2），特别是显微镜中，通过以下事实来感测漂移：第一次（T（n-1））获取不可移动的样本（30）的第一图像，并且其第二图像 （T（n））。 通过第一和第二图像之间的比较来计算漂移。

公开(公告)号：US20050046930A1

公开(公告)日：2005-03-03

申请号：US10499216

申请日：2002-12-09

Applicant: Frank Olschewski

Inventor： Frank Olschewski

IPC: G02B21/00 ,  G02B21/06 ,  G02B21/36 ,  G06T5/00 ,  G06T7/00

CPC classification number: G02B21/365 ,  G06T7/0002

Abstract: This invention comprises a method, a software, and a microscope system for monitoring and controlling information loss. The observation of information that is not present and the comparison of ideal loss to actual loss generates an explanatory component by means of a rule set. The user is instructed by the microscope system in an appropriate manner, for example by means of a display, to undertake actions that remove the defects.

Abstract translation: 本发明包括用于监测和控制信息丢失的方法，软件和显微镜系统。 不存在的信息的观察以及理想损失与实际损失的比较通过规则集产生说明性组件。 由显微镜系统以适当的方式（例如通过显示器）指示使用者进行消除缺陷的动作。

公开(公告)号：US06721690B2

公开(公告)日：2004-04-13

申请号：US09681051

申请日：2000-12-10

Applicant: Frank Olschewski

Inventor： Frank Olschewski

IPC: G06F300

CPC classification number: G02B21/16 ,  G02B21/002

Abstract: A method and system for analyzing acquired scan data are disclosed. The method involves measuring physical values of a particular process, converting the measured values into two signals, such as electrical signals, and then converting the two signals into polar coordinates by defining a vector having a radial magnitude and a vector angle corresponding to the magnitudes of the two signals. The method further provides for validation and visual representation of the measured physical values by employing the angle information to define the upper and lower bounds of the acceptable measured values. The corresponding system comprises sensors measuring the two physical values and yielding the two signals. A signal processing unit receives the two signals, converts them into a pair of polar coordinates defines by the magnitude of the two signals.

Abstract translation: 公开了一种用于分析采集的扫描数据的方法和系统。 该方法涉及测量特定过程的物理值，将测量值转换成两个信号，例如电信号，然后通过定义具有径向幅度和向量角度的向量将两个信号转换为极坐标 两个信号。 该方法还通过采用角度信息来定义可接受的测量值的上限​​和下限来提供所测量的物理值的验证和视觉表示。 相应的系统包括测量两个物理值并产生两个信号的传感器。 信号处理单元接收两个信号，将它们转换成由两个信号的大小定义的一对极坐标。