利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

6 条记录 (耗时 0.036 秒)

    Expression of factor VII and IX activities in mammalian cells
    1.
    发明授权
    Expression of factor VII and IX activities in mammalian cells 失效
    因子VII和IX活性在哺乳动物细胞中的表达

    公开(公告)号：US08603777B1

    公开(公告)日：2013-12-10

    申请号：US07765452

    申请日：1991-09-25

    申请人： Frederick S. Hagen ,  Mark J. Murray ,  Sharon J. Busby ,  Kathleen L. Berkner ,  Margaret Y. Insley ,  Richard G. Woodbury ,  Charles L. Gray

    发明人： Frederick S. Hagen ,  Mark J. Murray ,  Sharon J. Busby ,  Kathleen L. Berkner ,  Margaret Y. Insley ,  Richard G. Woodbury ,  Charles L. Gray

    IPC分类号： C12P21/02 ,  C12N5/10

    CPC分类号： C12N9/644 ,  C07K2319/00 ,  C12N2710/10043

    摘要： Methods are disclosed for producing proteins having biological activity for blood coagulation mediated by Factor VIIa or Factor IX. The proteins are produced by mammalian host cells which have been stably transfected with a DNA construct containing a nucleotide sequence which codes at least partially for either Factor VII or Factor IX. The nucleotide sequence comprises a first nucleotide sequence encoding a calcium binding domain, joined to a second nucleotide sequence positioned downstream of the first sequence. The second sequence encodes a catalytic domain for the serine protease activity of either Factor VIIa or Factor IX. The joined sequences code for proteins having substantially the same biological activity for blood coagulation as either Factor VIIa or Factor IX.

    摘要翻译： 公开了用于产生由因子VIIa或因子IX介导的凝血生物活性的蛋白质的方法。 哺乳动物宿主细胞产生的蛋白质已被含有至少部分为因子VII或因子IX编码的核苷酸序列的DNA构建体稳定转染。 核苷酸序列包含编​​码钙结合结构域的第一核苷酸序列，连接到位于第一序列下游的第二核苷酸序列。 第二个序列编码因子VIIa或因子IX的丝氨酸蛋白酶活性的催化结构域。 连接的序列编码与凝血因子VIIa或因子IX具有基本相同的生物活性的蛋白质。

    Transglutaminase cross-linkable polypeptides and methods relating thereto
    5.
    发明授权
    Transglutaminase cross-linkable polypeptides and methods relating thereto 失效
    转谷氨酰胺酶可交联多肽及其相关方法

    公开(公告)号：US5939385A

    公开(公告)日：1999-08-17

    申请号：US483236

    申请日：1995-06-07

    申请人： Virender Labroo ,  Sharon J. Busby

    发明人： Virender Labroo ,  Sharon J. Busby

    IPC分类号： A61L24/10 ,  C07K14/47 ,  C07K14/75 ,  A61K38/00

    CPC分类号： C07K14/75 ,  A61L24/106 ,  C07K14/4732

    摘要： Polypeptides of from about 9-120 amino acid residues comprising a truncated segment of fibrinogen which may be flanked by elastomeric peptides, wherein the polypeptides are cross-linkable by a transglutaminase, homo- and copolymers containing such polypeptides are disclosed. The homo- and co-polymers disclosed herein are useful in tissue sealant and wound healing formulations.

    摘要翻译： 包含约9-120个氨基酸残基的多肽，其包含纤维蛋白原的截短区段，其可以侧翼为弹性肽，其中所述多肽可通过转谷氨酰胺酶交联，包含此类多肽的均聚物和共聚物。 本文公开的均聚物和共聚物可用于组织密封剂和伤口愈合制剂。