公开(公告)号：US4738926A

公开(公告)日：1988-04-19

申请号：US709711

申请日：1985-03-08

申请人： Fukusaburo Hamada ,  Keishin Sugahara ,  Kou-ichi Shiosaki ,  Satoshi Adachi ,  Hiroshi Mizokami

发明人： Fukusaburo Hamada ,  Keishin Sugahara ,  Kou-ichi Shiosaki ,  Satoshi Adachi ,  Hiroshi Mizokami

IPC分类号： C12P21/00 ,  A61K39/00 ,  A61K39/29 ,  C07K14/02 ,  C12N7/02

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2730/10122 ,  Y10S435/803 ,  Y10S435/82 ,  Y10S530/826

摘要： Industrially useful method for the purification of HBs antigen produced by a recombinant organism being capable of producing HBs antigen which is prepared by means of DNA recombination technique, which comprises subjecting an HBs antigen-containing material produced by a recombinant organism to an adsorption chromatography with a silica, optionally followed by a gel filtration and further an adsorption chromatography with a hydroxyapatite, and then eluting the HBs antigen, preferably, with a buffer having a pH 9 or more which is incorporated with urea. The purification method can give a highly pure HBs antigen suitable for the preparation of hepatitis B vaccine in a large scale from recombinant organisms prepared by DNA recombination technique.

摘要翻译： 能够产生通过DNA重组技术制备的HBs抗原产生的HBs抗原产生的工业上有用的方法，该方法包括使由重组生物产生的含HBs抗原的材料进行吸附色谱， 二氧化硅，任选地随后进行凝胶过滤，并进一步用羟基磷灰石进行吸附色谱，然后优选用与尿素结合的pH9或更高的缓冲液洗脱HBs抗原。 纯化方法可以通过DNA重组技术制备重组生物体，从而大规模制备适合于乙型肝炎疫苗制备的高纯度HBs抗原。

公开(公告)号：US4612283A

公开(公告)日：1986-09-16

申请号：US656315

申请日：1984-10-01

申请人： Keishin Sugahara ,  Chikateru Nozaki ,  Fukusaburo Hamada ,  Fumio Miake ,  Nobuya Ohtomo

发明人： Keishin Sugahara ,  Chikateru Nozaki ,  Fukusaburo Hamada ,  Fumio Miake ,  Nobuya Ohtomo

IPC分类号： C12N15/09 ,  A61K39/00 ,  C07K1/22 ,  C07K14/005 ,  C07K14/02 ,  C07K14/10 ,  C07K14/18 ,  C07K14/195 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/865 ,  A61K39/29

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2730/10122

摘要： A method for purification of HBs antigen, which comprises treating an HBs tigen-containing material with an acid, optionally subjecting to salting out with ammonium sulfate, and subjecting the material to a chromatography with hydroxyapatite, by which a highly purified HBs antigen suitable for the preparation of HBV vaccine or diagnostic agent can be obtained in an industrial scale. This method is particularly useful for the purification of HBs antigen-containing material produced by recombinants obtained by DNA recombination technique.

摘要翻译： 一种用于纯化HBs抗原的方法，其包括用酸处理含HBs抗原的材料，任选地用硫酸铵进行盐析，并用羟基磷灰石进行色谱分离，通过该色谱法，适用于 可以以工业规模获得HBV疫苗或诊断剂的制备。 该方法对于通过DNA重组技术获得的重组体产生的含HBs抗原的材料的纯化特别有用。

公开(公告)号：US4839277A

公开(公告)日：1989-06-13

申请号：US811399

申请日：1985-12-20

申请人： Keishin Sugahara ,  Takayuki Imamura ,  Fukusaburo Hamada ,  Nobuya Ohtomo ,  Haruo Fujita ,  Kazuhide Yagami

发明人： Keishin Sugahara ,  Takayuki Imamura ,  Fukusaburo Hamada ,  Nobuya Ohtomo ,  Haruo Fujita ,  Kazuhide Yagami

IPC分类号： C07K14/02 ,  G01N33/576

CPC分类号： C07K14/005 ,  G01N33/5762 ,  C12N2730/10122 ,  Y10S436/82

摘要： A method for the purification of HBc antigen, particularly HBc antigen produced by recombinant organisms by means of DNA recombination technique, which comprises subjecting a solution containing HBc antigen to acid-treatment, wherein the solution containing HBc antigen is acidified to a pH range of not higher than 6 by adding an acid and then resulting precipitates of lipid and contaminant proteins are removed, and then subjecting the acid-treated solution containing HBc antigen to an ion exchange chromatography with an anion exchanger, and a method for measuring HBc antibody by using said purified HBc antigen in a passive hemagglutination method, EIA method or RIA method.

摘要翻译： 一种通过DNA重组技术纯化HBc抗原，特别是由重组生物产生的HBc抗原的方法，其包括使含有HBc抗原的溶液进行酸处理，其中将含有HBc抗原的溶液酸化至pH范围 通过添加酸高于6，然后除去所得到的脂质和污染蛋白质沉淀物，然后将含有HBc抗原的酸处理溶液用阴离子交换剂进行离子交换色谱，以及通过使用所述方法测量HBc抗体 被动血凝法纯化HBc抗原，EIA法或RIA法。