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公开(公告)号：US4738926A
公开(公告)日：1988-04-19
申请号：US709711
申请日：1985-03-08
CPC分类号： C07K14/005 , A61K39/00 , C12N2730/10122 , Y10S435/803 , Y10S435/82 , Y10S530/826
摘要： Industrially useful method for the purification of HBs antigen produced by a recombinant organism being capable of producing HBs antigen which is prepared by means of DNA recombination technique, which comprises subjecting an HBs antigen-containing material produced by a recombinant organism to an adsorption chromatography with a silica, optionally followed by a gel filtration and further an adsorption chromatography with a hydroxyapatite, and then eluting the HBs antigen, preferably, with a buffer having a pH 9 or more which is incorporated with urea. The purification method can give a highly pure HBs antigen suitable for the preparation of hepatitis B vaccine in a large scale from recombinant organisms prepared by DNA recombination technique.
摘要翻译： 能够产生通过DNA重组技术制备的HBs抗原产生的HBs抗原产生的工业上有用的方法，该方法包括使由重组生物产生的含HBs抗原的材料进行吸附色谱， 二氧化硅，任选地随后进行凝胶过滤，并进一步用羟基磷灰石进行吸附色谱，然后优选用与尿素结合的pH9或更高的缓冲液洗脱HBs抗原。 纯化方法可以通过DNA重组技术制备重组生物体，从而大规模制备适合于乙型肝炎疫苗制备的高纯度HBs抗原。
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公开(公告)号：US4710378A
公开(公告)日：1987-12-01
申请号：US709705
申请日：1985-03-08
CPC分类号： C07K14/005 , A61K39/00 , C12N2730/10122
摘要： Lyophilized preparation of hepartitis B vaccine, which comprises a purified hepatitis B virus surface antigen produced by a recombinant organism being capable of producing HBs antigen, which is adsorbed on aluminum gel in the lyophilized state in the presence of a stabilizer, said lyophilized preparation being prepared by the steps of adding an aluminum gel and a stabilizer to a purified recombinant-origin HBs antigen and lyophilizing the mixture. Said lyophilized preparation being able to be stably kept for a long period of time without losing its antigen titer and being useful for the prophylaxis of infection of hepatitis B virus.
摘要翻译： 所述肝炎B疫苗的冻干制剂包含由能够产生HBs抗原的重组生物产生的纯化乙型肝炎病毒表面抗原，其在稳定剂存在下以冻干状态吸附在铝凝胶上，所述冻干制剂被制备 通过在纯化的重组起始HBs抗原中加入铝凝胶和稳定剂并将混合物冻干的步骤。 所述冻干制剂能够长时间稳定地保持而不失去其抗原滴度，并且可用于预防乙型肝炎病毒感染。
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公开(公告)号：US07001885B2
公开(公告)日：2006-02-21
申请号：US10175382
申请日：2002-06-20
申请人： Satoshi Adachi , Hiroshi Mizokami , Yoshitaka Tajima , Yoshinobu Miyatsu , Toshinobu Nouchi , Yoshitaka Ushio
发明人： Satoshi Adachi , Hiroshi Mizokami , Yoshitaka Tajima , Yoshinobu Miyatsu , Toshinobu Nouchi , Yoshitaka Ushio
IPC分类号： A61K38/00
CPC分类号： C07K14/765 , C07K1/18
摘要： Multimers of human serum albumin are removed from a solution of human serum albumin containing the multimers by bringing the human serum albumin solution into contact with an anion-exchanger equilibrated with a buffer containing a salt in a concentration ranging from 10 to 150 mM and having a pH value ranging from 5 to 9.5.
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公开(公告)号：US07667009B2
公开(公告)日：2010-02-23
申请号：US10555883
申请日：2004-04-14
申请人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
发明人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
IPC分类号： A23J1/00
CPC分类号： C07K14/43531 , A61K38/00 , A61K39/00
摘要： A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.
摘要翻译： 提供了通过遗传重组技术获得的改良的主要螨变应原的纯化方法和通过所述纯化方法获得的纯化的改良的主要螨变应原。 一种用于纯化通过遗传重组技术获得的改性主要螨变应原的方法，其包括纯化步骤：（1）洗涤并回收含有通过MF膜的遗传重组技术获得的修饰的主要螨变应原的包含体; （2）溶解所述包涵体，然后重折叠; （3）浓缩含有改性主要螨变应原的溶液，同时用超滤膜除去低分子量成分; （4）用阴离子交换剂回收未吸附级分的改性主要螨变应原; （5）用疏水凝胶回收吸附级分中的改性主要螨变应原; 和（6）用阴离子交换剂回收吸附级分中的改性主要螨变应原，以及通过所述纯化方法获得的具有高纯度的改性主要螨变应原。
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公开(公告)号：US20080113424A1
公开(公告)日：2008-05-15
申请号：US10555883
申请日：2004-04-14
申请人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
发明人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
CPC分类号： C07K14/43531 , A61K38/00 , A61K39/00
摘要： A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.
摘要翻译： 提供了通过遗传重组技术获得的改良的主要螨变应原的纯化方法和通过所述纯化方法获得的纯化的改良的主要螨变应原。 一种用于纯化通过遗传重组技术获得的改良的主要螨变应原的方法，其包括纯化步骤：（1）洗涤并回收含有通过MF膜的遗传重组技术获得的改性主要螨变应原的包含体; （2）溶解所述包涵体，然后重折叠; （3）浓缩含有改性主要螨变应原的溶液，同时用超滤膜除去低分子量成分; （4）用阴离子交换剂回收未吸附级分的改性主要螨变应原; （5）用疏水凝胶回收吸附级分中的改性主要螨变应原; 和（6）用阴离子交换剂回收吸附级分中的改性主要螨变应原，以及通过所述纯化方法获得的具有高纯度的改性主要螨变应原。
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公开(公告)号：US4563303A
公开(公告)日：1986-01-07
申请号：US722381
申请日：1985-04-12
申请人： Akihiro Ginnaga , Shin Sakuma , Tsukasa Nishihara , Tomitaka Tashiro , Sadao Susumi , Tetsuo Kawahara , Hiroshi Mizokami
发明人： Akihiro Ginnaga , Shin Sakuma , Tsukasa Nishihara , Tomitaka Tashiro , Sadao Susumi , Tetsuo Kawahara , Hiroshi Mizokami
IPC分类号： C12P21/00 , A61K39/00 , C07K14/235 , A61K39/10
CPC分类号： C07K14/235 , A61K39/00 , Y10S435/822 , Y10S530/825
摘要： Improved method for the purification of filamentous hemagglutinin (F-HA) on industrial scale which comprises contacting a culture of a microorganism of the genus Bordetella with a cellulose sulfate gel, a polysaccharide gel chemically bound with dextran sulfate, or a crosslinked polysaccharide sulfate gel, thereby adsorbing F-HA on the gel, and then eluting F-HA from the gel. Said method can give a highly purified F-HA which does not contain any other proteins, lipid, saccharides, etc. and further undesirable endotoxin, and hence can be used for producing various reagents, medicines and pertussis vaccine.
摘要翻译： 用于工业规模净化丝状血凝素（F-HA）的改进方法，其包括将博德特氏菌属的微生物的培养物与硫酸纤维素凝胶接触，与硫酸葡聚糖化学结合的多糖凝胶或交联的多糖硫酸凝胶， 从而在凝胶上吸附F-HA，然后从凝胶中洗脱F-HA。 所述方法可以得到不含任何其它蛋白质，脂质，糖类等的高度纯化的F-HA，以及不希望的内毒素，因此可用于生产各种试剂，药物和百日咳疫苗。
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7.发明申请RECOMBINANT AVIAN INFECTIOUS CORYZA VACCINE AND PROCESS FOR PREPARING SAME 审中-公开重组AVIAN感染性CORYZA VACCINE及其制备方法公开(公告)号：US20130183330A1
公开(公告)日：2013-07-18
申请号：US13616255
申请日：2012-09-14
IPC分类号： A61K39/02
CPC分类号： A61K39/02 , A61K39/102 , C07K14/465
摘要： A recombinant avian infectious coryza vaccine and a process for preparing the same are provided. A process for preparing a recombinant avian infectious coryza vaccine which comprises step of constructing E. coli that may produce as an inclusion body a fusion peptide consisting of peptides derived from outer-membrane protein of Avibacterium paragarinarum serotype A and serotype C, step of culturing said E. coli and colleting and purifying inclusion body from culture, and step of preparing a preparation comprising said purified inclusion body, and an avian infectious coryza vaccine comprising as an active ingredient the fusion peptide. A linker sequence may be inserted between the respective peptides comprising the fusion peptide. For the peptide derived from the serotypes A and C, an amino acid sequence region of Region 2 or its vicinity responsible for protection from infection may be used.
摘要翻译： 提供了重组禽传染性鼻炎疫苗及其制备方法。 一种制备重组禽传染性鼻炎疫苗的方法，其包括构建可以产生作为包涵体的大肠杆菌的步骤，所述融合肽由衍生自副杆菌素A型血清型A和血清型C的外膜蛋白的肽组成，培养所述 大肠杆菌和来自培养物的收集和纯化包涵体，以及制备包含所述纯化的包涵体的制剂的步骤和包含作为活性成分的融合肽的禽传染性疫苗疫苗的步骤。 可以在包含融合肽的各个肽之间插入接头序列。 对于源自血清型A和C的肽，可以使用负责防止感染的区域2或其附近的氨基酸序列区域。
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公开(公告)号：US08332159B2
公开(公告)日：2012-12-11
申请号：US10546293
申请日：2004-02-18
IPC分类号： G06F7/00
CPC分类号： C07K16/00 , A61K2039/505 , C07K16/1063 , C07K2317/34
摘要： A method of enhancing the efficacy of a monoclonal antibody preparation wherein antigens from patients are tested for their reactivity with the antibody. An amino acid sequence of an expressed protein is deuced from a nucleotide sequence determined by isolation and analysis of a target molecule gene in a biopsy from a patient. This is compared with the previously determined amino acid sequence recognized by the monoclonal antibody preparation in order to assess the fitness of patients for administration of the monoclonal antibody preparation.
摘要翻译： 一种提高单克隆抗体制剂的功效的方法，其中测试来自患者的抗原与抗体的反应性。 通过从患者的活组织检查中分离和分析靶分子基因而确定的核苷酸序列，从表达的蛋白质的氨基酸序列中去除。 将其与由单克隆抗体制剂识别的先前确定的氨基酸序列进行比较，以评估患者施用单克隆抗体制剂的适合性。
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公开(公告)号：US06869934B2
公开(公告)日：2005-03-22
申请号：US10088588
申请日：2001-07-18
申请人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
发明人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
CPC分类号： C12N9/6432 , C07K14/4721 , C07K14/4728 , C12Y304/21006
摘要： The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.
摘要翻译： 本发明涉及通过阳离子交换层析纯化钙离子结合蛋白的方法。 本发明提供了从含有钙离子结合蛋白质和污染物的液体样品中以简单有效的方式分离和纯化钙离子结合蛋白的方法，而没有任何预处理如加入螯合剂。 更具体地，本发明涉及一种纯化钙离子结合蛋白的方法，其包括在钙离子存在下使所述蛋白质与阳离子交换载体接触以使所述蛋白质吸附到载体上，并且在洗涤后，洗脱 所述蛋白质和基本上不含有通过本发明的方法获得的污染物的钙离子结合蛋白质。
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公开(公告)号：US20050059805A1
公开(公告)日：2005-03-17
申请号：US10944810
申请日：2004-09-21
申请人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
发明人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
CPC分类号： C12N9/6432 , C07K14/4721 , C07K14/4728 , C12Y304/21006
摘要： The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.
摘要翻译： 本发明涉及通过阳离子交换层析纯化钙离子结合蛋白的方法。 本发明提供了从含有钙离子结合蛋白质和污染物的液体样品中以简单有效的方式分离和纯化钙离子结合蛋白的方法，而没有任何预处理如加入螯合剂。 更具体地，本发明涉及一种纯化钙离子结合蛋白的方法，其包括在钙离子存在下使所述蛋白质与阳离子交换载体接触以使所述蛋白质吸附到载体上，并且在洗涤后，洗脱 所述蛋白质和基本上不含有通过本发明的方法获得的污染物的钙离子结合蛋白质。
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