公开(公告)号：US20180120273A1

公开(公告)日：2018-05-03

申请号：US15721389

申请日：2017-09-29

Applicant: Genentech, Inc.

Inventor： Yajun WANG ,  George Tony MORENO ,  Boyan ZHANG ,  Liangyi ZHANG ,  Dell FARNAN ,  Thomas PATAPOFF

IPC: G01N30/96 ,  G01N33/68 ,  C07K1/18

CPC classification number: G01N30/96 ,  C07K1/18 ,  G01N33/6803

Abstract: The present invention provides methods for analyzing compositions of polypeptides such as antibodies by ionic strength-mediated pH gradient ion exchange chromatography. In some aspects, the methods use a combination of pH gradients and ionic strength gradients to separate the polypeptide from charge variants of the polypeptide. In some aspects, the methods use a stable ionic strength to optimize the pH gradient separation window to separate the polypeptide from charge variants. Such methods are useful for analyzing polypeptide, e.g. antibodies, with a pI greater than 9 or a pI less than 7. In some aspects, the invention provides a multiproduct method for the analysis of polypeptides of varying pI's.

公开(公告)号：US20130281355A1

公开(公告)日：2013-10-24

申请号：US13841864

申请日：2013-03-15

Applicant: GENENTECH, INC.

Inventor： Natarajan VIJAYASANKARAN ,  Steven J. MEIER ,  Melissa S. MUN ,  Sharat VARMA ,  Yi YANG ,  Boyan ZHANG ,  Silvana R. AREVALO ,  Martin GAWLITZEK ,  Veronica CARVALHAL

IPC: C12N5/00 ,  C07K16/00 ,  C12P21/00

CPC classification number: C12N5/0018 ,  C07K16/00 ,  C12N5/005 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2501/39 ,  C12P21/00

Abstract: Cell culture media, such as chemically defined cell culture media, are provided, as are methods of using the media for cell growth (i.e., cell culture) and polypeptide (e.g., antibody) production. Compositions comprising polypeptides produced by the methods are also provided.