公开(公告)号：US20210324098A1

公开(公告)日：2021-10-21

申请号：US17217706

申请日：2021-03-30

Applicant: Genentech, Inc.

Inventor： Martin GAWLITZEK ,  Shun LUO ,  Christina Teresa BEVILACQUA

IPC: C07K16/28 ,  C12N5/00 ,  C07K16/18 ,  C07K16/22 ,  C07K14/705

Abstract: The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.

公开(公告)号：US20170283505A1

公开(公告)日：2017-10-05

申请号：US15623226

申请日：2017-06-14

Applicant: Genentech, Inc.

Inventor： Martin GAWLITZEK ,  Shun LUO ,  Christina Teresa BEVILACQUA

IPC: C07K16/28 ,  C07K16/18 ,  C07K14/705 ,  C12N5/00 ,  C07K16/22

CPC classification number: C07K16/2878 ,  C07K14/70575 ,  C07K16/18 ,  C07K16/22 ,  C07K2317/14 ,  C07K2319/30 ,  C12N5/0018 ,  C12N5/0043 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/90

Abstract: The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.

公开(公告)号：US20190169667A1

公开(公告)日：2019-06-06

申请号：US16185369

申请日：2018-11-09

Applicant: GENENTECH, INC.

Inventor： Martin GAWLITZEK ,  Sven MARKERT ,  Oliver POPP ,  Masaru Ken SHIRATORI ,  Thomas TROEBS ,  Jessica WUU

IPC: C12P21/00 ,  C12N5/00 ,  C12N5/071

Abstract: Provided herein are cell culture media and methods culturing host cells expressing polypeptides to reduce the level of trisulfide bonds in polypeptides produced by the host cells.

公开(公告)号：US20190263921A1

公开(公告)日：2019-08-29

申请号：US16412791

申请日：2019-05-15

Applicant: Genentech, Inc.

Inventor： Martin GAWLITZEK ,  Shun LUO ,  Christina Teresa BEVILACQUA

IPC: C07K16/28 ,  C07K16/22 ,  C07K14/705 ,  C12N5/00 ,  C07K16/18

Abstract: The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.

公开(公告)号：US20160024217A1

公开(公告)日：2016-01-28

申请号：US14670079

申请日：2015-03-26

Applicant: GENENTECH, INC.

Inventor： Martin GAWLITZEK ,  Shun LUO ,  Christina Teresa PETRAGLIA

IPC: C07K16/28 ,  C07K16/22 ,  C07K14/705 ,  C12N5/00

CPC classification number: C07K16/2878 ,  C07K14/70575 ,  C07K16/18 ,  C07K16/22 ,  C07K2317/14 ,  C07K2319/30 ,  C12N5/0018 ,  C12N5/0043 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/90

Abstract: The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.

Abstract translation: 本发明一般涉及补充天冬酰胺的不含谷氨酰胺的细胞培养基。 本发明还涉及在天冬酰胺补充的不含谷氨酰胺的哺乳动物细胞培养物中重组蛋白质如抗体的产生。

公开(公告)号：US20130281355A1

公开(公告)日：2013-10-24

申请号：US13841864

申请日：2013-03-15

Applicant: GENENTECH, INC.

Inventor： Natarajan VIJAYASANKARAN ,  Steven J. MEIER ,  Melissa S. MUN ,  Sharat VARMA ,  Yi YANG ,  Boyan ZHANG ,  Silvana R. AREVALO ,  Martin GAWLITZEK ,  Veronica CARVALHAL

IPC: C12N5/00 ,  C07K16/00 ,  C12P21/00

CPC classification number: C12N5/0018 ,  C07K16/00 ,  C12N5/005 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2501/39 ,  C12P21/00

Abstract: Cell culture media, such as chemically defined cell culture media, are provided, as are methods of using the media for cell growth (i.e., cell culture) and polypeptide (e.g., antibody) production. Compositions comprising polypeptides produced by the methods are also provided.

公开(公告)号：US20250136705A1

公开(公告)日：2025-05-01

申请号：US18820925

申请日：2024-08-30

Applicant: Genentech, Inc.

Inventor： Martin GAWLITZEK ,  Shun LUO ,  Christina Teresa BEVILACQUA

IPC: C07K16/28 ,  C07K14/705 ,  C07K16/18 ,  C07K16/22 ,  C12N5/00

Abstract: The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.

公开(公告)号：US20250066493A1

公开(公告)日：2025-02-27

申请号：US18942174

申请日：2024-11-08

Applicant: Genentech, Inc.

Inventor： Martin GAWLITZEK ,  Shun LUO ,  Christina Teresa BEVILACQUA

IPC: C07K16/28 ,  C07K14/705 ,  C07K16/18 ,  C07K16/22 ,  C12N5/00

Abstract: The present invention relates generally to glutamine-free cell culture media supplemented with arginine. The invention further concerns the production of recombinant proteins, such as antibodies, in arginine-supplemented glutamine-free mammalian cell culture.

公开(公告)号：US20190023800A1

公开(公告)日：2019-01-24

申请号：US16138704

申请日：2018-09-21

Applicant: Genentech, Inc.

Inventor： Martin GAWLITZEK ,  Shun LUO ,  Christina Teresa BEVILACQUA

IPC: C07K16/28 ,  C07K16/22 ,  C12N5/00 ,  C07K14/705 ,  C07K16/18

Abstract: The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.

公开(公告)号：US20180148510A1

公开(公告)日：2018-05-31

申请号：US15881308

申请日：2018-01-26

Applicant: Genentech, Inc.

Inventor： Martin GAWLITZEK ,  Shun LUO ,  Christina Teresa BEVILACQUA

IPC: C07K16/28 ,  C12N5/00 ,  C07K16/22 ,  C07K16/18 ,  C07K14/705

CPC classification number: C07K16/2878 ,  C07K14/70575 ,  C07K16/18 ,  C07K16/22 ,  C07K2317/14 ,  C07K2319/30 ,  C12N5/0018 ,  C12N5/0043 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/90

Abstract: The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.