公开(公告)号：US20190292591A1

公开(公告)日：2019-09-26

申请号：US16351456

申请日：2019-03-12

Applicant: GENIA TECHNOLOGIES, INC. ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy GEISER ,  Randall DAVIS ,  Roger CHEN

IPC: C12Q1/6874 ,  C12Q1/6869 ,  B82Y15/00

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.

公开(公告)号：US20190085386A1

公开(公告)日：2019-03-21

申请号：US15809725

申请日：2017-11-10

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Randall DAVIS ,  Roger CHEN

IPC: C12Q1/6869 ,  G01N33/487 ,  G01N33/543 ,  C12N15/11

Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

公开(公告)号：US20190249244A1

公开(公告)日：2019-08-15

申请号：US16396284

申请日：2019-04-26

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Randall DAVIS ,  Roger CHEN

IPC: C12Q1/6869 ,  G01N33/487 ,  C12N15/11 ,  G01N33/543

CPC classification number: C12Q1/6869 ,  B01J2219/00313 ,  B01J2219/00653 ,  B01J2219/00734 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/16 ,  C12N2310/3517 ,  C12N2320/10 ,  C12N2320/32 ,  C12Q2565/607 ,  C12Q2565/631 ,  G01N33/48721 ,  G01N33/5432

Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and insert-ing the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

公开(公告)号：US20160327507A1

公开(公告)日：2016-11-10

申请号：US15066117

申请日：2016-03-10

Applicant: Genia Technologies, Inc.

Inventor： Randall W. DAVIS ,  Edward Shian LIU ,  Eric Takeshi HARADA ,  Anne AGUIRRE ,  Andrew TRANS ,  James POLLARD ,  Cynthia CECH ,  Hui TIAN ,  Robert YUAN ,  John FOSTER ,  Roger CHEN

IPC: G01N27/327 ,  C12Q1/68 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.

公开(公告)号：US20150337366A1

公开(公告)日：2015-11-26

申请号：US14376836

申请日：2013-02-15

Applicant: Genia Technologies, Inc.

Inventor： Randall DAVIS ,  Roger CHEN

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/16 ,  C12N2310/3517 ,  C12N2320/10 ,  C12N2320/32 ,  G01N33/48721 ,  G01N33/5432 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

Abstract translation: 本公开提供了用于制造生物芯片的生物芯片和方法。 生物芯片可以包括邻近或接近电极的膜（例如脂质双层）中的纳米孔。 描述了形成膜并将纳米孔插入膜中的方法。 生物芯片和方法可用于核酸（例如DNA）测序。 本公开还描述了使用生物芯片检测，分选和分类分子（例如蛋白质）的方法。