公开(公告)号：US20230399688A1

公开(公告)日：2023-12-14

申请号：US18120951

申请日：2023-03-13

Applicant: GENOMATICA, INC.

Inventor： Stephanie CULLER ,  Ihsiung Brandon CHEN ,  Priti PHARKYA ,  Steve VAN DIEN ,  Nelson BARTON

IPC: C12Q1/6869 ,  A61K35/66 ,  C12P21/02 ,  C12P19/34 ,  A61K36/02 ,  A61K36/06 ,  C12N5/16 ,  C12N15/09 ,  C40B40/04

CPC classification number: C12Q1/6869 ,  A61K35/66 ,  C12P21/02 ,  C12P19/34 ,  A61K36/02 ,  A61K36/06 ,  C12N5/16 ,  C12N15/09 ,  C40B40/04 ,  Y02A50/30 ,  A61K2236/15 ,  A61K2236/30 ,  C12N2330/50

Abstract: In alternative embodiments, provided herein are transcription/translation (TX-TL) systems and methods of using them for use as rapid prototyping platforms for the synthesis, modification and identification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites, from biosynthetic gene cluster pipelines. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the combinatorial biosynthesis of natural products (NPs), natural product analogs (NPAs) and secondary metabolites. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the rapid prototyping of complex biosynthetic pathways as a way to rapidly assess combinatorial and biosynthetic designs before moving to cellular hosts. In alternative embodiments, these exemplary TX-TL systems are multiplexed for high-throughput (HT) automation and for prototyping engineered platforms for the synthesis or modification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites analogs.

公开(公告)号：US20180237847A1

公开(公告)日：2018-08-23

申请号：US15752884

申请日：2016-08-19

Applicant: GENOMATICA, INC.

Inventor： Stephanie CULLER ,  Ihsiung Brandon CHEN ,  Priti PHARKYA ,  Steve VAN DIEN ,  Nelson BARTON

IPC: C12Q1/6869 ,  C12N15/09 ,  C12N5/16 ,  A61K36/02 ,  A61K35/66 ,  A61K36/06 ,  C40B40/04

CPC classification number: C12Q1/6869 ,  A61K35/66 ,  A61K36/02 ,  A61K36/06 ,  A61K2236/15 ,  A61K2236/30 ,  C12N5/16 ,  C12N15/09 ,  C12N2330/50 ,  C12P19/34 ,  C12P21/02 ,  C40B40/04 ,  Y02A50/409 ,  Y02A50/469 ,  A61K35/12

Abstract: In alternative embodiments, provided herein are transcription/translation (TX-TL) systems and methods of using them for use as rapid prototyping platforms for the synthesis, modification and identification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites, from biosynthetic gene cluster pipelines. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the combinatorial biosynthesis of natural products (NPs), natural product analogs (NPAs) and secondary metabolites. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the rapid prototyping of complex biosynthetic pathways as a way to rapidly assess combinatorial and biosynthetic designs before moving to cellular hosts. In alternative embodiments, these exemplary TX-TL systems are multiplexed for high-throughput (HT) automation and for prototyping engineered platforms for the synthesis or modification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites analogs.