公开(公告)号：US20190010479A1

公开(公告)日：2019-01-10

申请号：US15578488

申请日：2016-05-26

Applicant: GENOMATICA, INC. ,  BRASKEM S.A.

Inventor： Stephanie J. CULLER ,  Robert J. HASELBECK ,  Harish NAGARAJAN ,  Iuri Estrada GOUVEA ,  Daniel Johannes KOCH ,  Mateus Schreiner Garcez LOPES ,  Lucas Pedersen PARIZZI

IPC: C12N9/88 ,  C12P7/16 ,  C07K16/40 ,  C12N15/82 ,  C08F36/06 ,  C12P5/02

Abstract: In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.

公开(公告)号：US20230279365A1

公开(公告)日：2023-09-07

申请号：US18084888

申请日：2022-12-20

Applicant: Braskem S.A.

Inventor： Verônica Leite QUEIROZ ,  Lucas Pedersen PARIZZI ,  Iuri Estrada GOUVEA ,  Debora Noma OKAMOTO ,  Rafael Victório Carvalho GUIDO ,  Alessandro Silva NASCIMENTO ,  Igor POLIKARPOV

IPC: C12N9/04 ,  C12N15/52

CPC classification number: C12N9/0006 ,  C12N15/52 ,  C12Y101/01001

Abstract: The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.

公开(公告)号：US20200283806A1

公开(公告)日：2020-09-10

申请号：US16796417

申请日：2020-02-20

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Lucas Pedersen PARIZZI ,  Felipe GALZERANI

IPC: C12P7/18 ,  C12P17/02 ,  C12N15/52 ,  C12N1/20 ,  C12P7/28 ,  C12P5/02

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG), or optionally MEG and one or more co-product, from one or more hexose feedstock. The present application also relates to recombinant microorganisms useful in the biosynthesis of glycolic acid (GA), or optionally GA and one or more co-product, from one or more hexose feedstock. The present application relates to recombinant microorganisms useful in the biosynthesis of xylitol, or optionally xylitol and one or more co-product, from one or more hexose feedstock. Also provided are methods of producing MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product, from one or more hexose feedstock using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product.

公开(公告)号：US20190309266A1

公开(公告)日：2019-10-10

申请号：US16378209

申请日：2019-04-08

Applicant: Braskem S.A.

Inventor： Verônica Leite QUEIROZ ,  Lucas Pedersen PARIZZI ,  Iuri Estrada GOUVEA ,  Debora Noma OKAMOTO ,  Rafael Victório Carvalho GUIDO ,  Alessandro Silva NASCIMENTO ,  Igor POLIKARPOV

IPC: C12N9/04 ,  C12N15/52

Abstract: The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.

公开(公告)号：US20240392329A1

公开(公告)日：2024-11-28

申请号：US18737619

申请日：2024-06-07

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Lucas Pedersen PARIZZI ,  Felipe GALZERANI

IPC: C12P7/18 ,  C12N1/20 ,  C12N15/52 ,  C12P5/02 ,  C12P7/28 ,  C12P17/02 ,  C12R1/19

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG), or optionally MEG and one or more co-product, from one or more hexose feedstock. The present application also relates to recombinant microorganisms useful in the biosynthesis of glycolic acid (GA), or optionally GA and one or more co-product, from one or more hexose feedstock. The present application relates to recombinant microorganisms useful in the biosynthesis of xylitol, or optionally xylitol and one or more co-product, from one or more hexose feedstock. Also provided are methods of producing MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product, from one or more hexose feedstock using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product.

公开(公告)号：US20200318146A1

公开(公告)日：2020-10-08

申请号：US16841312

申请日：2020-04-06

Applicant: Braskem S.A.

Inventor： Veronica Maria Rodege Gogola KOLLING ,  Ane Fernanda Beraldi ZEIDLER ,  Lucas Pedersen PARIZZI

IPC: C12P7/18 ,  C12N1/20

Abstract: The present disclosure provides methods for genetically modifying microbes to produce a microbe capable of simultaneous consumption of xylose and glucose to increase the productivity output of desired chemical products. The disclosure further provides modified bacteria that are capable of simultaneous consumption of xylose and glucose, and compositions comprising the microbes.

公开(公告)号：US20200208160A1

公开(公告)日：2020-07-02

申请号：US16728509

申请日：2019-12-27

Applicant: Braskem S.A.

Inventor： Ane Fernanda Beraldi ZEIDLER ,  Beatriz Leite MAGALHAES ,  Lucas Pedersen PARIZZI ,  Veronica Maria Rodege Gogola KOLLING

IPC: C12N15/52 ,  C12P7/28

Abstract: The present disclosure provides methods of modulating the flux of carbon through the monoethylene glycol (MEG) biosynthesis pathway and one or more C3 compound biosynthesis pathways by expressing enzymes that are essential for improving C3 compounds and modulating other genetic aspects of MEG and C3 compound biosynthesis. The disclosure is further drawn to modified microbes comprising the disrupted sequences and overexpressed sequences, and compositions thereof.

公开(公告)号：US20170260551A1

公开(公告)日：2017-09-14

申请号：US15453094

申请日：2017-03-08

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Mateus Schreiner LOPES ,  Ane Fernanda Beraldi ZEIDLER ,  Lucas Pedersen PARIZZI

IPC: C12P7/28 ,  C12P7/26 ,  C12P7/04 ,  C12P5/02 ,  C12N9/10 ,  C12N9/90 ,  C12N9/12 ,  C12N9/88 ,  C12N9/02 ,  C12P7/18 ,  C12N15/52

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.