公开(公告)号：US12018044B2

公开(公告)日：2024-06-25

申请号：US17157150

申请日：2021-01-25

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Francesco Berlanda Scorza ,  Yingxia Wen ,  Andrew Geall ,  Frederick Porter

IPC: C12Q1/68 ,  B01D15/36 ,  B01D15/38 ,  B01D61/14 ,  C07H1/06 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/10

CPC classification number: C07H21/02 ,  B01D15/36 ,  B01D15/38 ,  B01D15/3847 ,  B01D61/14 ,  C07H1/06 ,  C07H21/00 ,  C07H21/04 ,  C12N15/101 ,  C12N15/1017 ,  B01D2015/3838

Abstract: Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.

公开(公告)号：US11155572B2

公开(公告)日：2021-10-26

申请号：US14775760

申请日：2014-03-13

Applicant: GLAXOSMITHKLINE BIOLOGICALS sa

Inventor： Francesco Berlanda Scorza ,  Yingxia Wen ,  Andrew Geall ,  Frederick Porter

IPC: C12Q1/68 ,  C07H21/02 ,  B01D61/14 ,  B01D15/38 ,  C12N15/10 ,  B01D15/36 ,  C07H1/06

Abstract: Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.