公开(公告)号：US12018044B2

公开(公告)日：2024-06-25

申请号：US17157150

申请日：2021-01-25

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Francesco Berlanda Scorza ,  Yingxia Wen ,  Andrew Geall ,  Frederick Porter

IPC: C12Q1/68 ,  B01D15/36 ,  B01D15/38 ,  B01D61/14 ,  C07H1/06 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/10

CPC classification number: C07H21/02 ,  B01D15/36 ,  B01D15/38 ,  B01D15/3847 ,  B01D61/14 ,  C07H1/06 ,  C07H21/00 ,  C07H21/04 ,  C12N15/101 ,  C12N15/1017 ,  B01D2015/3838

Abstract: Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.

公开(公告)号：US11155572B2

公开(公告)日：2021-10-26

申请号：US14775760

申请日：2014-03-13

Applicant: GLAXOSMITHKLINE BIOLOGICALS sa

Inventor： Francesco Berlanda Scorza ,  Yingxia Wen ,  Andrew Geall ,  Frederick Porter

IPC: C12Q1/68 ,  C07H21/02 ,  B01D61/14 ,  B01D15/38 ,  C12N15/10 ,  B01D15/36 ,  C07H1/06

Abstract: Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.

公开(公告)号：US09683022B2

公开(公告)日：2017-06-20

申请号：US14410461

申请日：2013-06-29

Applicant: GlaxoSmithKline Biologicals SA

Inventor： Andrea Carfi ,  Yingxia Wen

IPC: C07K14/005 ,  A61K39/12 ,  A61K39/245 ,  C12N7/00 ,  A61K39/00

CPC classification number: C07K14/005 ,  A61K39/12 ,  A61K39/245 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/55588 ,  A61K2039/572 ,  C12N7/00 ,  C12N2710/16122 ,  C12N2710/16134 ,  C12N2710/16151

Abstract: An isolated human cytomegalovirus (HCMV) membrane protein complex that comprises gH, gL and at least one more HCMV glycoprotein is provided. In some embodiments the complex consists of gH, gL and gO. In other embodiments the complex consists of gH, gL, pUL128, pUL130 and pUL131A. Processes for expressing and purifying such complexes, and subsequent uses of such complexes in immunogenic compositions and vaccines, are also provided.