公开(公告)号：US11339367B2

公开(公告)日：2022-05-24

申请号：US15094077

申请日：2016-04-08

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Christiane Gerke ,  Francesco Berlanda Scorza ,  Allan Saul ,  Luana Maggiore

IPC: C12N1/20 ,  C07K14/25 ,  A61K39/00 ,  A61K35/12

Abstract: Hyperblebbing Shigella strains are generated by disrupting one or more components of the Tol-Pal system. The blebs from these strains are useful immunogens for vaccination. The individual proteins found in these blebs can also be used as immunogens.

公开(公告)号：US10035826B2

公开(公告)日：2018-07-31

申请号：US15150204

申请日：2016-05-09

Applicant: GlaxoSmithKline Biologicals SA ,  J. Craig Venter Institute, Inc.

Inventor： Vega Masignani ,  Danilo Gomes Moriel ,  Francesco Berlanda Scorza ,  Nathalie Norais ,  Maria Rita Fontana ,  Mariagrazia Pizza ,  Laura Serino ,  Herve Tettelin

IPC: A61K39/02 ,  C07K14/245 ,  C07K16/12 ,  A61K39/108 ,  A61K39/00

CPC classification number: C07K14/245 ,  A61K39/00 ,  A61K39/0258 ,  A61K2039/505 ,  A61K2039/523 ,  C07K16/1232

Abstract: Disclosed herein are various open reading frames from a strain of E. coli responsible for neonatal meningitis (MNEC), and a subset of these that is of particular interest for preparing compositions for immunizing against MNEC infections.

公开(公告)号：US12018044B2

公开(公告)日：2024-06-25

申请号：US17157150

申请日：2021-01-25

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Francesco Berlanda Scorza ,  Yingxia Wen ,  Andrew Geall ,  Frederick Porter

IPC: C12Q1/68 ,  B01D15/36 ,  B01D15/38 ,  B01D61/14 ,  C07H1/06 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/10

CPC classification number: C07H21/02 ,  B01D15/36 ,  B01D15/38 ,  B01D15/3847 ,  B01D61/14 ,  C07H1/06 ,  C07H21/00 ,  C07H21/04 ,  C12N15/101 ,  C12N15/1017 ,  B01D2015/3838

Abstract: Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.

公开(公告)号：US11155572B2

公开(公告)日：2021-10-26

申请号：US14775760

申请日：2014-03-13

Applicant: GLAXOSMITHKLINE BIOLOGICALS sa

Inventor： Francesco Berlanda Scorza ,  Yingxia Wen ,  Andrew Geall ,  Frederick Porter

IPC: C12Q1/68 ,  C07H21/02 ,  B01D61/14 ,  B01D15/38 ,  C12N15/10 ,  B01D15/36 ,  C07H1/06

Abstract: Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.