公开(公告)号：US06677506B1

公开(公告)日：2004-01-13

申请号：US09701068

申请日：2000-11-24

申请人： Gad Galili ,  Orit Shaul ,  Dirk Inze ,  Marc Van Montagu ,  Donald W. Hilgemann

发明人： Gad Galili ,  Orit Shaul ,  Dirk Inze ,  Marc Van Montagu ,  Donald W. Hilgemann

IPC分类号： A01H500

CPC分类号： C12N15/8273 ,  C07K14/415

摘要： An isolated DNA molecule is provided coding for a polypeptide of the 11-12 transmembrane domain transporter family having a Mg2+/H+ or Zn2+/H+ exchange activity, herein designated MHX. The genomic MHX DNA was isolated from Arabidopsis thaliana cv. C-24. Transgenic plants transformed with said DNA and expressing MHX are shown to have a lower content of sodium as compared with corresponding wild-type plants or a higher dry matter weight upon growth in calcium-rich media as compared with corresponding wild-type plants. These transgenic plants are tolerant to stress conditions, particularly high salinity and calcium-rich media, e.g. saline and calcareous soils.

摘要翻译： 提供分离的DNA分子，其编码具有Mg 2+ / H 2+或Zn 2+ 2+ / H +交换活性的11-12跨膜结构域转运蛋白家族的多肽，这里称为MHX。 从拟南芥cv。中分离出基因组MHX DNA。 C-24。 与相应的野生型植物相比，用所述DNA转化并表达MHX的转基因植物显示与相应的野生型植物相比具有较低的钠含量或较富钙培养基生长时更高的干物质重量。 这些转基因植物耐受胁迫条件，特别是高盐度和富含钙的培养基，例如， 盐水和钙质土壤。

公开(公告)号：US5367110A

公开(公告)日：1994-11-22

申请号：US792466

申请日：1991-11-13

申请人： Gad Galili ,  Orit Shaul ,  Avihai Perl

发明人： Gad Galili ,  Orit Shaul ,  Avihai Perl

IPC分类号： C12N9/12 ,  C12N9/88 ,  C12N15/54 ,  C12N15/60 ,  C12N15/82 ,  C12P13/08 ,  A01H1/04 ,  C12P21/04

CPC分类号： C12N15/8254 ,  C12N15/8251 ,  C12N15/8253 ,  C12N9/12 ,  C12N9/88 ,  C12P13/08

摘要： A chimeric gene construct comprising a DNA sequence encoding an enzyme having aspartate kinase (AK) activity is provided, which is capable of expression in plant cells with subsequent increased production of threonine. Transgenic plants containing in their cells said chimeric gene overproduce threonine and transgenic plants containing in their cells said chimeric gene and a second chimeric gene comprising a DNA sequence coding for an enzyme having dihydrodipicolinate synthase (DHPS) activity, overproduce both threonine and lysine. The transgenic plants are resistant to lysine and threonine, to derivatives thereof and to selective inhibitors of the plant enzymes DHPS or AK, and thus these compounds may be used as selective herbicides in locus where the transgenic plants are cultivated.

摘要翻译： 提供了包含编码具有天冬氨酸激酶（AK）活性的酶的DNA序列的嵌合基因构建体，其能够在随后增加的苏氨酸的产生中在植物细胞中表达。 在其细胞中含有转基因植物，所述嵌合基因过量产生苏氨酸和在其细胞中含有所述嵌合基因的转基因植物，以及包含编码具有二氢吡啶二羧酸合酶（DHPS）活性的酶的DNA序列的第二嵌合基因，其过量产生苏氨酸和赖氨酸。 转基因植物对赖氨酸和苏氨酸，其衍生物和植物酶DHPS或AK的选择性抑制剂具有抗性，因此这些化合物可用作培养转基因植物的基因座中的选择性除草剂。